Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market

In the present study a simple and highly sensitive RP-HPLC method has been established for simultaneous determination of chlorogenic acid, caffeic acid, vanillic acid and caffeine in coffee samples. The method has been applied to eight different coffees available on the Romanian market which were pr...

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Main Authors: Ion Trandafir, Violeta Nour, Mira Elena Ionica
Format: Article
Language:English
Published: Sociedad Latinoamericana de Nutrición
Series:Archivos Latinoamericanos de Nutrición
Subjects:
Online Access:http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0004-06222013000100011&lng=en&tlng=en
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author Ion Trandafir
Violeta Nour
Mira Elena Ionica
author_facet Ion Trandafir
Violeta Nour
Mira Elena Ionica
author_sort Ion Trandafir
collection DOAJ
description In the present study a simple and highly sensitive RP-HPLC method has been established for simultaneous determination of chlorogenic acid, caffeic acid, vanillic acid and caffeine in coffee samples. The method has been applied to eight different coffees available on the Romanian market which were previously analysed concerning the total polyphenols content and antioxidant capacity. Reduction of the DPPH radical was used to determine the antioxidant capacity of the coffee extracts while the total polyphenols content was determined by spectrophotometry (Folin Ciocalteu's method). The total polyphenols content ranged from 1.98 g GAE/100 g to 4.19 g GAE/100 g while the caffeine content ranged from 1.89 g/100 g to 3.05 g/100 g. A large variability was observed in chlorogenic acid content of the investigated coffee samples which ranged between 0.6 and 2.32 g/100 g.
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spelling doaj.art-a46ca97e6e5d43919d0c713a4c9c80fc2023-01-02T02:29:51ZengSociedad Latinoamericana de NutriciónArchivos Latinoamericanos de Nutrición0004-06226318794S0004-06222013000100011Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian marketIon Trandafir0Violeta NourMira Elena IonicaUniversity of CraiovaIn the present study a simple and highly sensitive RP-HPLC method has been established for simultaneous determination of chlorogenic acid, caffeic acid, vanillic acid and caffeine in coffee samples. The method has been applied to eight different coffees available on the Romanian market which were previously analysed concerning the total polyphenols content and antioxidant capacity. Reduction of the DPPH radical was used to determine the antioxidant capacity of the coffee extracts while the total polyphenols content was determined by spectrophotometry (Folin Ciocalteu's method). The total polyphenols content ranged from 1.98 g GAE/100 g to 4.19 g GAE/100 g while the caffeine content ranged from 1.89 g/100 g to 3.05 g/100 g. A large variability was observed in chlorogenic acid content of the investigated coffee samples which ranged between 0.6 and 2.32 g/100 g.http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0004-06222013000100011&lng=en&tlng=enácidos fenólicoscafeínahplccapacidad antioxidantecafé
spellingShingle Ion Trandafir
Violeta Nour
Mira Elena Ionica
Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market
Archivos Latinoamericanos de Nutrición
ácidos fenólicos
cafeína
hplc
capacidad antioxidante
café
title Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market
title_full Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market
title_fullStr Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market
title_full_unstemmed Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market
title_short Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market
title_sort antioxidant capacity phenolic acids and caffeine contents of some commercial coffees available on the romanian market
topic ácidos fenólicos
cafeína
hplc
capacidad antioxidante
café
url http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0004-06222013000100011&lng=en&tlng=en
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AT miraelenaionica antioxidantcapacityphenolicacidsandcaffeinecontentsofsomecommercialcoffeesavailableontheromanianmarket