Soil sampling methods for microbial study in montane regions
Soil microorganism studies are crucial in ecological and biological research. However, the lack of standardized analytical methods for sampling makes it challenging to compare studies and obtain consistent results. Therefore, it is essential to establish standardized sampling methods in the field. O...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Global Ecology and Conservation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2351989423003141 |
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| author | She Rong Qi Fu-Liang Chen Yi-Ting Zhou Fa-Ping Deng Wei Lu Ya-Xian Huang Zhi-Pang Yang Xiao-Yan Xiao Wen |
| author_facet | She Rong Qi Fu-Liang Chen Yi-Ting Zhou Fa-Ping Deng Wei Lu Ya-Xian Huang Zhi-Pang Yang Xiao-Yan Xiao Wen |
| author_sort | She Rong |
| collection | DOAJ |
| description | Soil microorganism studies are crucial in ecological and biological research. However, the lack of standardized analytical methods for sampling makes it challenging to compare studies and obtain consistent results. Therefore, it is essential to establish standardized sampling methods in the field. Our study used a stratification method to collect 120 soil samples from various altitudinal ranges on Cangshan Mountain in China, with an altitude of 4122 m, and incredible microorganism biodiversity. This site provides an ideal landscape for studying elevation patterns in microorganisms. We conducted analyses on the variations of depth and stratification used in the sampling process, as these factors have been found to impact microbial diversity, elevation patterns, and community assembly mechanisms. We found that increasing the sampling depth provided more valuable research results by revealing elevation patterns of soil microorganisms. We therefore recommend sampling to a depth of 35 cm with stratification to determine the overall soil microbial diversity. A depth of 25 cm is suitable for studying elevation patterns with stratification. For investigating community assembly mechanisms without stratification, a depth of 15 cm is recommended. When exploring a specific taxon, the sampling depth can be adjusted accordingly. For studying the elevation pattern of archaea, a depth of 20 cm is appropriate. In the case of fungi, a depth of 40 cm is recommended, while a depth of 20 cm is suitable for studying elevation patterns. This study emphasizes the importance of standardization in sampling methods, particularly along the soil profiles, when studying soil microbiomes in montane areas. These recommendations should be adopted in future research to achieve more consistent and reliable results in the field. |
| first_indexed | 2024-03-11T16:05:20Z |
| format | Article |
| id | doaj.art-a46cd7e8700d4996a4bf57475163ed95 |
| institution | Directory Open Access Journal |
| issn | 2351-9894 |
| language | English |
| last_indexed | 2024-03-11T16:05:20Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Global Ecology and Conservation |
| spelling | doaj.art-a46cd7e8700d4996a4bf57475163ed952023-10-25T04:16:24ZengElsevierGlobal Ecology and Conservation2351-98942023-11-0147e02679Soil sampling methods for microbial study in montane regionsShe Rong0Qi Fu-Liang1Chen Yi-Ting2Zhou Fa-Ping3Deng Wei4Lu Ya-Xian5Huang Zhi-Pang6Yang Xiao-Yan7Xiao Wen8Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China; Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali, Yunnan 671003, China; The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Dali University, Dali, Yunnan 671003, China; Yunling Black-and-white Snub-nosed Monkey Observation and Research Station of Yunnan Province, Dali, Yunnan 671003, ChinaInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China; Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali, Yunnan 671003, China; The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Dali University, Dali, Yunnan 671003, China; Corresponding authors at: Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China.Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China; Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali, Yunnan 671003, China; The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Dali University, Dali, Yunnan 671003, China; Yunling Black-and-white Snub-nosed Monkey Observation and Research Station of Yunnan Province, Dali, Yunnan 671003, China; Corresponding authors at: Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China.Soil microorganism studies are crucial in ecological and biological research. However, the lack of standardized analytical methods for sampling makes it challenging to compare studies and obtain consistent results. Therefore, it is essential to establish standardized sampling methods in the field. Our study used a stratification method to collect 120 soil samples from various altitudinal ranges on Cangshan Mountain in China, with an altitude of 4122 m, and incredible microorganism biodiversity. This site provides an ideal landscape for studying elevation patterns in microorganisms. We conducted analyses on the variations of depth and stratification used in the sampling process, as these factors have been found to impact microbial diversity, elevation patterns, and community assembly mechanisms. We found that increasing the sampling depth provided more valuable research results by revealing elevation patterns of soil microorganisms. We therefore recommend sampling to a depth of 35 cm with stratification to determine the overall soil microbial diversity. A depth of 25 cm is suitable for studying elevation patterns with stratification. For investigating community assembly mechanisms without stratification, a depth of 15 cm is recommended. When exploring a specific taxon, the sampling depth can be adjusted accordingly. For studying the elevation pattern of archaea, a depth of 20 cm is appropriate. In the case of fungi, a depth of 40 cm is recommended, while a depth of 20 cm is suitable for studying elevation patterns. This study emphasizes the importance of standardization in sampling methods, particularly along the soil profiles, when studying soil microbiomes in montane areas. These recommendations should be adopted in future research to achieve more consistent and reliable results in the field.http://www.sciencedirect.com/science/article/pii/S2351989423003141StandardizationSampling methodMicrobiologyDiversityElevation patternAssembly mechanism |
| spellingShingle | She Rong Qi Fu-Liang Chen Yi-Ting Zhou Fa-Ping Deng Wei Lu Ya-Xian Huang Zhi-Pang Yang Xiao-Yan Xiao Wen Soil sampling methods for microbial study in montane regions Global Ecology and Conservation Standardization Sampling method Microbiology Diversity Elevation pattern Assembly mechanism |
| title | Soil sampling methods for microbial study in montane regions |
| title_full | Soil sampling methods for microbial study in montane regions |
| title_fullStr | Soil sampling methods for microbial study in montane regions |
| title_full_unstemmed | Soil sampling methods for microbial study in montane regions |
| title_short | Soil sampling methods for microbial study in montane regions |
| title_sort | soil sampling methods for microbial study in montane regions |
| topic | Standardization Sampling method Microbiology Diversity Elevation pattern Assembly mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S2351989423003141 |
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