Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat
Abstract Background Vancomycin-resistant enterococci (VRE) are among the most common causative pathogens for nosocomial infections worldwide. Moreover, strains of VRE have been isolated from several domestic livestock in Egypt. Methods This study examined if healthy dogs are a potential source of VR...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-10-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-023-09708-4 |
| _version_ | 1797577900544753664 |
|---|---|
| author | Khaled A. Abd El-Razik Eman S. Ibrahim Amany A. Arafa Riham H. Hedia Abdelgayed M. Younes Mahmoud H. Hasanain |
| author_facet | Khaled A. Abd El-Razik Eman S. Ibrahim Amany A. Arafa Riham H. Hedia Abdelgayed M. Younes Mahmoud H. Hasanain |
| author_sort | Khaled A. Abd El-Razik |
| collection | DOAJ |
| description | Abstract Background Vancomycin-resistant enterococci (VRE) are among the most common causative pathogens for nosocomial infections worldwide. Moreover, strains of VRE have been isolated from several domestic livestock in Egypt. Methods This study examined if healthy dogs are a potential source of VRE infection by isolating and characterizing Enterococcus faecium strains from stool samples on a morphological basis and biochemical activities. Subsequently, it was confirmed by genotypic characterization using polymerase chain reaction (PCR), followed by the detection of antibiotic resistance genes, virulence determinants, and genes contributing to enterocin production by PCR. Furthermore, the phylogenetic relationships among vanB and tetL genes were analyzed. Results All ten fecal samples were identified as E. faecium and confirmed by PCR. In addition, 90% of the isolates tested were positive for the virulence genes gelE and esp, and all the isolates tested were positive for the antibiotic resistance genes tetL and vanB. Only three of the five enterocin genes examined were detected. Ent As-48, bacteriocin 31, and Ent L50 were identified in 100%, 80%, and 60% of the samples, respectively. Conclusion Dogs should be regarded as a reservoir of E. faecium that carries vancomycin resistance and virulence determinants that may affect public health in Egypt, considering a "One Health" task force approach to restrict their spread. |
| first_indexed | 2024-03-10T22:15:37Z |
| format | Article |
| id | doaj.art-a4783961226843feab3913db7ac4b0b3 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-10T22:15:37Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-a4783961226843feab3913db7ac4b0b32023-11-19T12:28:16ZengBMCBMC Genomics1471-21642023-10-0124111110.1186/s12864-023-09708-4Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threatKhaled A. Abd El-Razik0Eman S. Ibrahim1Amany A. Arafa2Riham H. Hedia3Abdelgayed M. Younes4Mahmoud H. Hasanain5Department of Animal Reproduction, Veterinary Research Institute, National Research CentreDepartment of Microbiology and Immunology, Veterinary Research Institute, National Research CentreDepartment of Microbiology and Immunology, Veterinary Research Institute, National Research CentreDepartment of Microbiology and Immunology, Veterinary Research Institute, National Research CentreDepartment of Hydrobiology, Veterinary Research Institute, National Research CentreDepartment of Animal Reproduction, Veterinary Research Institute, National Research CentreAbstract Background Vancomycin-resistant enterococci (VRE) are among the most common causative pathogens for nosocomial infections worldwide. Moreover, strains of VRE have been isolated from several domestic livestock in Egypt. Methods This study examined if healthy dogs are a potential source of VRE infection by isolating and characterizing Enterococcus faecium strains from stool samples on a morphological basis and biochemical activities. Subsequently, it was confirmed by genotypic characterization using polymerase chain reaction (PCR), followed by the detection of antibiotic resistance genes, virulence determinants, and genes contributing to enterocin production by PCR. Furthermore, the phylogenetic relationships among vanB and tetL genes were analyzed. Results All ten fecal samples were identified as E. faecium and confirmed by PCR. In addition, 90% of the isolates tested were positive for the virulence genes gelE and esp, and all the isolates tested were positive for the antibiotic resistance genes tetL and vanB. Only three of the five enterocin genes examined were detected. Ent As-48, bacteriocin 31, and Ent L50 were identified in 100%, 80%, and 60% of the samples, respectively. Conclusion Dogs should be regarded as a reservoir of E. faecium that carries vancomycin resistance and virulence determinants that may affect public health in Egypt, considering a "One Health" task force approach to restrict their spread.https://doi.org/10.1186/s12864-023-09708-4Enterococcus faeciumDogsTetracyclineVancomycinBacteriocinsOne health |
| spellingShingle | Khaled A. Abd El-Razik Eman S. Ibrahim Amany A. Arafa Riham H. Hedia Abdelgayed M. Younes Mahmoud H. Hasanain Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat BMC Genomics Enterococcus faecium Dogs Tetracycline Vancomycin Bacteriocins One health |
| title | Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat |
| title_full | Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat |
| title_fullStr | Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat |
| title_full_unstemmed | Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat |
| title_short | Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat |
| title_sort | molecular characterization of tetracycline and vancomycin resistant enterococcus faecium isolates from healthy dogs in egypt a public health threat |
| topic | Enterococcus faecium Dogs Tetracycline Vancomycin Bacteriocins One health |
| url | https://doi.org/10.1186/s12864-023-09708-4 |
| work_keys_str_mv | AT khaledaabdelrazik molecularcharacterizationoftetracyclineandvancomycinresistantenterococcusfaeciumisolatesfromhealthydogsinegyptapublichealththreat AT emansibrahim molecularcharacterizationoftetracyclineandvancomycinresistantenterococcusfaeciumisolatesfromhealthydogsinegyptapublichealththreat AT amanyaarafa molecularcharacterizationoftetracyclineandvancomycinresistantenterococcusfaeciumisolatesfromhealthydogsinegyptapublichealththreat AT rihamhhedia molecularcharacterizationoftetracyclineandvancomycinresistantenterococcusfaeciumisolatesfromhealthydogsinegyptapublichealththreat AT abdelgayedmyounes molecularcharacterizationoftetracyclineandvancomycinresistantenterococcusfaeciumisolatesfromhealthydogsinegyptapublichealththreat AT mahmoudhhasanain molecularcharacterizationoftetracyclineandvancomycinresistantenterococcusfaeciumisolatesfromhealthydogsinegyptapublichealththreat |