Safety of transtympanic application of probiotics in a chinchilla animal model
Abstract Background Chronic suppurative otitis media can be recalcitrant and difficult to treat, particularly with the increasing occurrence of antibiotic resistance. Lactobacillus plantarum is a probiotic that has been shown to decrease S. aureus and P. aeruginosa growth in wounds, making it a good...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40463-017-0242-y |
| _version_ | 1797967390699421696 |
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| author | Carol Nhan Aren Bezdjian Shyamali Saha Satya Prakash Lily H. P. Nguyen Sam J. Daniel |
| author_facet | Carol Nhan Aren Bezdjian Shyamali Saha Satya Prakash Lily H. P. Nguyen Sam J. Daniel |
| author_sort | Carol Nhan |
| collection | DOAJ |
| description | Abstract Background Chronic suppurative otitis media can be recalcitrant and difficult to treat, particularly with the increasing occurrence of antibiotic resistance. Lactobacillus plantarum is a probiotic that has been shown to decrease S. aureus and P. aeruginosa growth in wounds, making it a good candidate for the treatment of chronic suppurative otitis media. However, before it can be applied in the ear, its ototoxicity potential must be evaluated. Methods A prospective controlled trial was conducted in a chinchilla animal model at the Animal care research facilities of the Montreal Children’s Hospital Research Institute to determine whether Lactobacillus plantarum is ototoxic when applied transtympanically. Ten chinchillas each had one ear randomly assigned to receive 109 CFU/mL of Lactobacillus plantarum solution, while the contralateral ear received saline. Auditory brainstem responses were measured bilaterally at 8, 20, 25 kHz before, at 7–10 days after application, and at 28 days after application of probiotic or saline. Facial nerve and vestibular function were assessed clinically. Results There were no statistically significant differences in hearing thresholds between control and experimental ears at 28 days after application. A difference of 11 dB was noted in the 25 kHz range at day 7–10, but resolved by day 28. No animals receiving probiotics developed vestibular nerve dysfunction. There was no histologic evidence of auditory hair cell damaged evidenced by scanning electron microscopy. Conclusion Our study suggests that a single application of Lactobacillus plantarum at 109 CFU/mL does not cause ototoxicity in a chinchilla animal model. These preliminary safety evaluations and the pathogen inhibitory effects of L. plantarum demonstrated by previous studies present this probiotic as a candidate of interest for further investigation. |
| first_indexed | 2024-04-11T02:29:23Z |
| format | Article |
| id | doaj.art-a47eaef0a3f84f82bb137534a03ff9b9 |
| institution | Directory Open Access Journal |
| issn | 1916-0216 |
| language | English |
| last_indexed | 2024-04-11T02:29:23Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj.art-a47eaef0a3f84f82bb137534a03ff9b92023-01-02T21:42:29ZengBMCJournal of Otolaryngology - Head and Neck Surgery1916-02162017-11-014611610.1186/s40463-017-0242-ySafety of transtympanic application of probiotics in a chinchilla animal modelCarol Nhan0Aren Bezdjian1Shyamali Saha2Satya Prakash3Lily H. P. Nguyen4Sam J. Daniel5Department of Otolaryngology – Head & Neck Surgery and McGill Auditory Sciences Laboratory, The Montréal Children’s Hospital, McGill UniversityDepartment of Experimental Surgery and McGill Auditory Sciences Laboratory, The Montréal Children’s Hospital, McGill UniversityDepartments of Biomedical Engineering, Physiology, and Artificial Cells and Organs Research Center, Biomedical Technology and Cell Therapy Research Laboratory, McGill UniversityDepartments of Biomedical Engineering, Physiology, and Artificial Cells and Organs Research Center, Biomedical Technology and Cell Therapy Research Laboratory, McGill UniversityDepartment of Otolaryngology – Head & Neck Surgery and Center for Medical Education, McGill UniversityDepartment of Otolaryngology – Head & Neck Surgery and McGill Auditory Sciences Laboratory, The Montréal Children’s Hospital, McGill UniversityAbstract Background Chronic suppurative otitis media can be recalcitrant and difficult to treat, particularly with the increasing occurrence of antibiotic resistance. Lactobacillus plantarum is a probiotic that has been shown to decrease S. aureus and P. aeruginosa growth in wounds, making it a good candidate for the treatment of chronic suppurative otitis media. However, before it can be applied in the ear, its ototoxicity potential must be evaluated. Methods A prospective controlled trial was conducted in a chinchilla animal model at the Animal care research facilities of the Montreal Children’s Hospital Research Institute to determine whether Lactobacillus plantarum is ototoxic when applied transtympanically. Ten chinchillas each had one ear randomly assigned to receive 109 CFU/mL of Lactobacillus plantarum solution, while the contralateral ear received saline. Auditory brainstem responses were measured bilaterally at 8, 20, 25 kHz before, at 7–10 days after application, and at 28 days after application of probiotic or saline. Facial nerve and vestibular function were assessed clinically. Results There were no statistically significant differences in hearing thresholds between control and experimental ears at 28 days after application. A difference of 11 dB was noted in the 25 kHz range at day 7–10, but resolved by day 28. No animals receiving probiotics developed vestibular nerve dysfunction. There was no histologic evidence of auditory hair cell damaged evidenced by scanning electron microscopy. Conclusion Our study suggests that a single application of Lactobacillus plantarum at 109 CFU/mL does not cause ototoxicity in a chinchilla animal model. These preliminary safety evaluations and the pathogen inhibitory effects of L. plantarum demonstrated by previous studies present this probiotic as a candidate of interest for further investigation.http://link.springer.com/article/10.1186/s40463-017-0242-yProbioticOtitisOtotoxicityTranstympanicChinchilla |
| spellingShingle | Carol Nhan Aren Bezdjian Shyamali Saha Satya Prakash Lily H. P. Nguyen Sam J. Daniel Safety of transtympanic application of probiotics in a chinchilla animal model Journal of Otolaryngology - Head and Neck Surgery Probiotic Otitis Ototoxicity Transtympanic Chinchilla |
| title | Safety of transtympanic application of probiotics in a chinchilla animal model |
| title_full | Safety of transtympanic application of probiotics in a chinchilla animal model |
| title_fullStr | Safety of transtympanic application of probiotics in a chinchilla animal model |
| title_full_unstemmed | Safety of transtympanic application of probiotics in a chinchilla animal model |
| title_short | Safety of transtympanic application of probiotics in a chinchilla animal model |
| title_sort | safety of transtympanic application of probiotics in a chinchilla animal model |
| topic | Probiotic Otitis Ototoxicity Transtympanic Chinchilla |
| url | http://link.springer.com/article/10.1186/s40463-017-0242-y |
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