Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma
Introduction: Inflammatory cells may influence the growth of malignant neoplasms through pro and anti-tumour effects. Natural Killer (NK) cells and cytotoxic T lymphocytes (CD8+) are the main effector cells against cancer. Aim: This study aimed to evaluate the presence of NK cells and CD8+ T ly...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/10956/29455_CE[Ra]_F(Sh)_PF1(SK_BT_SS)_PFA(MJ_AnG).pdf |
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| author | Thais Aline Oliveira Maciel Marianna Sampaio Serpa Rodrigo Porpino Mafra Amanda Katarinny Goes Gonzaga Lélia Batista de Souza Leão Pereira Pinto |
| author_facet | Thais Aline Oliveira Maciel Marianna Sampaio Serpa Rodrigo Porpino Mafra Amanda Katarinny Goes Gonzaga Lélia Batista de Souza Leão Pereira Pinto |
| author_sort | Thais Aline Oliveira Maciel |
| collection | DOAJ |
| description | Introduction: Inflammatory cells may influence the growth
of malignant neoplasms through pro and anti-tumour effects.
Natural Killer (NK) cells and cytotoxic T lymphocytes (CD8+) are
the main effector cells against cancer.
Aim: This study aimed to evaluate the presence of NK cells and
CD8+ T lymphocytes in lower lip Squamous Cell Carcinomas
(SCCs) and its correlation with clinico-pathologic parameters.
Materials and Methods: Thirty two cases of lower lip SCCs were
selected for clinical, morphologic and Immunohistochemical
(IHC) assessment. NK cells (CD57+) and CD8+ T lymphocytes
were identified and analysed quantitatively in five microscopic
fields at the tumour invasion front using IHC markers.
Results: All cases showed immunopositivity for both biomarkers.
CD57+ and CD8+ T cells were more abundant in clinical stages
I/II, nonmetastatic cases and low-grade malignancy tumours.
However, there were no statistically significant differences.
Similarly, no significant differences were found regarding local
recurrence and clinical outcome. A strong positive correlation,
statistically significant between CD57+ and CD8+ T cells was
also observed (r=0.761, p<0.001).
Conclusion: The high expression of CD57+ and CD8+ cells
at the invasion front suggests they are involved in anti-tumour
defense. However, these cells do not seem to effectively
influence the progression of lower lip SCC. This may partially
reflect the activities of other components of the immune system
in the tumour microenvironment. |
| first_indexed | 2024-04-12T05:33:30Z |
| format | Article |
| id | doaj.art-a4802ab6d4ad45e7899832c217912b92 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T05:33:30Z |
| publishDate | 2017-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-a4802ab6d4ad45e7899832c217912b922022-12-22T03:45:57ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-12-011112EC22EC2510.7860/JCDR/2017/29455.10956Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell CarcinomaThais Aline Oliveira Maciel0Marianna Sampaio Serpa1Rodrigo Porpino Mafra2Amanda Katarinny Goes Gonzaga3 Lélia Batista de Souza4Leão Pereira Pinto5Postgraduate Student, Department of Dentistry, University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.Postgraduate Student, Centro Internacional de Pesquisa, A.C. Camargo Cancer Center, São Paulo, Brazil.Postgraduate Student, Department of Dentistry, University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.Postgraduate Student, Department of Dentistry, University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.Professor, Department of Dentistry, University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.Professor, Department of Dentistry, University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.Introduction: Inflammatory cells may influence the growth of malignant neoplasms through pro and anti-tumour effects. Natural Killer (NK) cells and cytotoxic T lymphocytes (CD8+) are the main effector cells against cancer. Aim: This study aimed to evaluate the presence of NK cells and CD8+ T lymphocytes in lower lip Squamous Cell Carcinomas (SCCs) and its correlation with clinico-pathologic parameters. Materials and Methods: Thirty two cases of lower lip SCCs were selected for clinical, morphologic and Immunohistochemical (IHC) assessment. NK cells (CD57+) and CD8+ T lymphocytes were identified and analysed quantitatively in five microscopic fields at the tumour invasion front using IHC markers. Results: All cases showed immunopositivity for both biomarkers. CD57+ and CD8+ T cells were more abundant in clinical stages I/II, nonmetastatic cases and low-grade malignancy tumours. However, there were no statistically significant differences. Similarly, no significant differences were found regarding local recurrence and clinical outcome. A strong positive correlation, statistically significant between CD57+ and CD8+ T cells was also observed (r=0.761, p<0.001). Conclusion: The high expression of CD57+ and CD8+ cells at the invasion front suggests they are involved in anti-tumour defense. However, these cells do not seem to effectively influence the progression of lower lip SCC. This may partially reflect the activities of other components of the immune system in the tumour microenvironment.https://jcdr.net/articles/PDF/10956/29455_CE[Ra]_F(Sh)_PF1(SK_BT_SS)_PFA(MJ_AnG).pdfimmunohistochemistrylip cancert8 lymphocytes |
| spellingShingle | Thais Aline Oliveira Maciel Marianna Sampaio Serpa Rodrigo Porpino Mafra Amanda Katarinny Goes Gonzaga Lélia Batista de Souza Leão Pereira Pinto Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma Journal of Clinical and Diagnostic Research immunohistochemistry lip cancer t8 lymphocytes |
| title | Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma |
| title_full | Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma |
| title_fullStr | Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma |
| title_full_unstemmed | Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma |
| title_short | Immunohistochemical Analysis of Natural Killer Cells and CD8+ T Lymphocytes in Lower Lip Squamous Cell Carcinoma |
| title_sort | immunohistochemical analysis of natural killer cells and cd8 t lymphocytes in lower lip squamous cell carcinoma |
| topic | immunohistochemistry lip cancer t8 lymphocytes |
| url | https://jcdr.net/articles/PDF/10956/29455_CE[Ra]_F(Sh)_PF1(SK_BT_SS)_PFA(MJ_AnG).pdf |
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