Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
Microplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about the link between obesity and exposure to environmental pollutants, including MP...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12421 |
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| author | Aden Geonhee Lee Sora Kang Hye Ji Yoon Suyeol Im Seung Jun Oh Youngmi Kim Pak |
| author_facet | Aden Geonhee Lee Sora Kang Hye Ji Yoon Suyeol Im Seung Jun Oh Youngmi Kim Pak |
| author_sort | Aden Geonhee Lee |
| collection | DOAJ |
| description | Microplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about the link between obesity and exposure to environmental pollutants, including MPs. In this study, we conducted a comprehensive investigation utilizing in silico, in vitro, and in vivo approaches to explore the brain distribution and physiological effects of MPs. Molecular docking simulations were performed to assess the binding affinity of three plastic polymers (ethylene, propylene, and styrene) to immune cells (macrophages, CD4<sup>+</sup>, and CD8<sup>+</sup> lymphocytes). The results revealed that styrene exhibited the highest binding affinity for macrophages. Furthermore, in vitro experiments employing fluorescence-labeled PS-MPs (fPS-MPs) of 1 μm at various concentrations demonstrated a dose-dependent binding of fPS-MPs to BV2 murine microglial cells. Subsequent oral administration of fPS-MPs to high-fat diet-induced obese mice led to the co-existence of fPS-MPs with immune cells in the blood, exacerbating impaired glucose metabolism and insulin resistance and promoting systemic inflammation. Additionally, fPS-MPs were detected throughout the brain, with increased activation of microglia in the hypothalamus. These findings suggest that PS-MPs significantly contribute to the exacerbation of systemic inflammation in high-fat diet-induced obesity by activating peripheral and central inflammatory immune cells. |
| first_indexed | 2024-03-11T00:25:40Z |
| format | Article |
| id | doaj.art-a482c5f483084905888b796f2dc8e0f7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:25:40Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a482c5f483084905888b796f2dc8e0f72023-11-18T23:04:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124151242110.3390/ijms241512421Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced ObesityAden Geonhee Lee0Sora Kang1Hye Ji Yoon2Suyeol Im3Seung Jun Oh4Youngmi Kim Pak5Phillips Exeter Academy, Exeter, NH 03833, USADepartment of Neuroscience, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Neuroscience, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Neuroscience, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaMicroplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about the link between obesity and exposure to environmental pollutants, including MPs. In this study, we conducted a comprehensive investigation utilizing in silico, in vitro, and in vivo approaches to explore the brain distribution and physiological effects of MPs. Molecular docking simulations were performed to assess the binding affinity of three plastic polymers (ethylene, propylene, and styrene) to immune cells (macrophages, CD4<sup>+</sup>, and CD8<sup>+</sup> lymphocytes). The results revealed that styrene exhibited the highest binding affinity for macrophages. Furthermore, in vitro experiments employing fluorescence-labeled PS-MPs (fPS-MPs) of 1 μm at various concentrations demonstrated a dose-dependent binding of fPS-MPs to BV2 murine microglial cells. Subsequent oral administration of fPS-MPs to high-fat diet-induced obese mice led to the co-existence of fPS-MPs with immune cells in the blood, exacerbating impaired glucose metabolism and insulin resistance and promoting systemic inflammation. Additionally, fPS-MPs were detected throughout the brain, with increased activation of microglia in the hypothalamus. These findings suggest that PS-MPs significantly contribute to the exacerbation of systemic inflammation in high-fat diet-induced obesity by activating peripheral and central inflammatory immune cells.https://www.mdpi.com/1422-0067/24/15/12421polystyrenemicroplasticsBV2 cellshigh-fat dietobesityinflammation |
| spellingShingle | Aden Geonhee Lee Sora Kang Hye Ji Yoon Suyeol Im Seung Jun Oh Youngmi Kim Pak Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity International Journal of Molecular Sciences polystyrene microplastics BV2 cells high-fat diet obesity inflammation |
| title | Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity |
| title_full | Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity |
| title_fullStr | Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity |
| title_full_unstemmed | Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity |
| title_short | Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity |
| title_sort | polystyrene microplastics exacerbate systemic inflammation in high fat diet induced obesity |
| topic | polystyrene microplastics BV2 cells high-fat diet obesity inflammation |
| url | https://www.mdpi.com/1422-0067/24/15/12421 |
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