Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients
The FilmArray® Pneumonia plus Panel (FAPP) is a new multiplex molecular test for hospital-acquired pneumonia (HAP), which can rapidly detect 18 bacteria, 9 viruses, and 7 resistance genes. We aimed to compare the diagnosis performance of FAPP with conventional testing in 100 intensive care unit (ICU...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.02080/full |
| _version_ | 1828523695367258112 |
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| author | Lise Crémet Lise Crémet Benjamin Gaborit Benjamin Gaborit Marwan Bouras Marwan Bouras Thomas Drumel Florian Guillotin Cécile Poulain Elise Persyn Karim Lakhal Bertrand Rozec Marie-Anne Vibet Antoine Roquilly Antoine Roquilly Sophie Gibaud |
| author_facet | Lise Crémet Lise Crémet Benjamin Gaborit Benjamin Gaborit Marwan Bouras Marwan Bouras Thomas Drumel Florian Guillotin Cécile Poulain Elise Persyn Karim Lakhal Bertrand Rozec Marie-Anne Vibet Antoine Roquilly Antoine Roquilly Sophie Gibaud |
| author_sort | Lise Crémet |
| collection | DOAJ |
| description | The FilmArray® Pneumonia plus Panel (FAPP) is a new multiplex molecular test for hospital-acquired pneumonia (HAP), which can rapidly detect 18 bacteria, 9 viruses, and 7 resistance genes. We aimed to compare the diagnosis performance of FAPP with conventional testing in 100 intensive care unit (ICU) patients who required mechanical ventilation, with clinically suspected HAP. A total of 237 samples [76 bronchoalveolar lavages (BALDS) and 82 endotracheal aspirates (ETADS) obtained at HAP diagnosis, and 79 ETA obtained during follow-up (ETATT)], were analyzed independently by routine microbiology testing and FAPP. 58 patients had paired BALDS and ETADS. The positivity thresholds of semi-quantified bacteria were 103–104 CFUs/mL or 104 copies/mL for BAL, and 105 CFUs/mL or copies/mL for ETA. Respiratory commensals (H. influenzae, S. aureus, E. coli, S. pneumoniae) were the most common pathogens. Discordant results for bacterial identification were observed in 33/76 (43.4%) BALDS and 36/82 (43.9%) ETADS, and in most cases, FAPP identified one supplemental bacteria (23/33 BALDS and 21/36 ETADS). An absence of growth, or polybacterial cultures, explained almost equally the majority of the non-detections in culture. No linear relationship was observed between bin and CFUs/mL variables. Concordant results between paired BALDS and ETADS were obtained in 46/58 (79.3%) patients with FAPP. One of the 17 resistance genes detected with FAPP (mecA/C and MREJ) was not confirmed by conventional testing. Overall, FAPP enhanced the positivity rate of diagnostic testing, with increased recognition of coinfections. Implementing this strategy may allow clinicians to make more timely and informed decisions. |
| first_indexed | 2024-12-11T20:33:41Z |
| format | Article |
| id | doaj.art-a482cfea7c5a41cd934037c649642ac5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T20:33:41Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a482cfea7c5a41cd934037c649642ac52022-12-22T00:51:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-08-011110.3389/fmicb.2020.02080577715Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit PatientsLise Crémet0Lise Crémet1Benjamin Gaborit2Benjamin Gaborit3Marwan Bouras4Marwan Bouras5Thomas Drumel6Florian Guillotin7Cécile Poulain8Elise Persyn9Karim Lakhal10Bertrand Rozec11Marie-Anne Vibet12Antoine Roquilly13Antoine Roquilly14Sophie Gibaud15Service de Bactériologie-Hygiène, Pôle de Biologie, CHU Nantes, Nantes, FranceLaboratoire UPRES EA3826, IRS2 – Nantes Biotech, Université de Nantes, Nantes, FranceLaboratoire UPRES EA3826, IRS2 – Nantes Biotech, Université de Nantes, Nantes, FranceService de Maladies Infectieuses et Tropicales et CIC 1413, CHU Nantes, Nantes, FranceLaboratoire UPRES EA3826, IRS2 – Nantes Biotech, Université de Nantes, Nantes, FranceService d’Anesthésie Réanimation Chirurgicale, Pôle Anesthésie-Réanimation, CHU Nantes, Nantes, FranceService de Bactériologie-Hygiène, Pôle de Biologie, CHU Nantes, Nantes, FranceService d’Anesthésie Réanimation Chirurgicale, Pôle Anesthésie-Réanimation, CHU Nantes, Nantes, FranceService d’Anesthésie Réanimation Chirurgicale, Pôle Anesthésie-Réanimation, CHU Nantes, Nantes, FranceService de Bactériologie-Hygiène, Pôle de Biologie, CHU Nantes, Nantes, FranceService de Réanimation en Chirurgie Polyvalente, Pôle Anesthésie-Réanimation, Hôpital Nord Laennec, CHU Nantes, Nantes, FranceService de Réanimation en Chirurgie Cardio-Thoracique et Vasculaire, Pôle Anesthésie-Réanimation, Hôpital Nord Laennec, CHU Nantes, Nantes, FrancePlateforme de Méthodologie et Biostatistique, CHU Nantes, Nantes, FranceLaboratoire UPRES EA3826, IRS2 – Nantes Biotech, Université de Nantes, Nantes, FranceService d’Anesthésie Réanimation Chirurgicale, Pôle Anesthésie-Réanimation, CHU Nantes, Nantes, FranceService de Bactériologie-Hygiène, Pôle de Biologie, CHU Nantes, Nantes, FranceThe FilmArray® Pneumonia plus Panel (FAPP) is a new multiplex molecular test for hospital-acquired pneumonia (HAP), which can rapidly detect 18 bacteria, 9 viruses, and 7 resistance genes. We aimed to compare the diagnosis performance of FAPP with conventional testing in 100 intensive care unit (ICU) patients who required mechanical ventilation, with clinically suspected HAP. A total of 237 samples [76 bronchoalveolar lavages (BALDS) and 82 endotracheal aspirates (ETADS) obtained at HAP diagnosis, and 79 ETA obtained during follow-up (ETATT)], were analyzed independently by routine microbiology testing and FAPP. 58 patients had paired BALDS and ETADS. The positivity thresholds of semi-quantified bacteria were 103–104 CFUs/mL or 104 copies/mL for BAL, and 105 CFUs/mL or copies/mL for ETA. Respiratory commensals (H. influenzae, S. aureus, E. coli, S. pneumoniae) were the most common pathogens. Discordant results for bacterial identification were observed in 33/76 (43.4%) BALDS and 36/82 (43.9%) ETADS, and in most cases, FAPP identified one supplemental bacteria (23/33 BALDS and 21/36 ETADS). An absence of growth, or polybacterial cultures, explained almost equally the majority of the non-detections in culture. No linear relationship was observed between bin and CFUs/mL variables. Concordant results between paired BALDS and ETADS were obtained in 46/58 (79.3%) patients with FAPP. One of the 17 resistance genes detected with FAPP (mecA/C and MREJ) was not confirmed by conventional testing. Overall, FAPP enhanced the positivity rate of diagnostic testing, with increased recognition of coinfections. Implementing this strategy may allow clinicians to make more timely and informed decisions.https://www.frontiersin.org/article/10.3389/fmicb.2020.02080/fullmultiplex syndromic testinghospital-acquired pneumoniarapid diagnosiscoinfectionantibiotic resistance |
| spellingShingle | Lise Crémet Lise Crémet Benjamin Gaborit Benjamin Gaborit Marwan Bouras Marwan Bouras Thomas Drumel Florian Guillotin Cécile Poulain Elise Persyn Karim Lakhal Bertrand Rozec Marie-Anne Vibet Antoine Roquilly Antoine Roquilly Sophie Gibaud Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients Frontiers in Microbiology multiplex syndromic testing hospital-acquired pneumonia rapid diagnosis coinfection antibiotic resistance |
| title | Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients |
| title_full | Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients |
| title_fullStr | Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients |
| title_full_unstemmed | Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients |
| title_short | Evaluation of the FilmArray® Pneumonia Plus Panel for Rapid Diagnosis of Hospital-Acquired Pneumonia in Intensive Care Unit Patients |
| title_sort | evaluation of the filmarray r pneumonia plus panel for rapid diagnosis of hospital acquired pneumonia in intensive care unit patients |
| topic | multiplex syndromic testing hospital-acquired pneumonia rapid diagnosis coinfection antibiotic resistance |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.02080/full |
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