Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals
Objectives: Here, we show two probable haplotypes associated with the human leukocyte antigen (HLA) DRB1*13:50:01 allele. The haplotypes were observed from 41 randomized unrelated Taiwanese individuals among a population of 23,064 individuals tested. Materials and Methods: The samples in this study...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Tzu-Chi Medical Journal |
| Subjects: | |
| Online Access: | http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2021;volume=33;issue=4;spage=370;epage=373;aulast=Mazibuko |
| _version_ | 1818695779457433600 |
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| author | Nondumiso Ayanda Mazibuko Kuo-Liang Yang |
| author_facet | Nondumiso Ayanda Mazibuko Kuo-Liang Yang |
| author_sort | Nondumiso Ayanda Mazibuko |
| collection | DOAJ |
| description | Objectives: Here, we show two probable haplotypes associated with the human leukocyte antigen (HLA) DRB1*13:50:01 allele. The haplotypes were observed from 41 randomized unrelated Taiwanese individuals among a population of 23,064 individuals tested. Materials and Methods: The samples in this study were blood samples, preserved in dipotassium ethylenediaminetetraacetic acid and/or ACD anticoagulants. The population is of donors from Tzu Chi Bone Marrow Donor Registry. Allele typing was performed using the sequence-based typing method, Sanger's sequencing. To discern the HLA-A and HLA-B alleles, exons 2 and 3 were sequenced. For DRB1 alleles, exon 2 was sequenced. Target exon sequence amplifications were done by a polymerase chain reaction and the resulting amplicons were sequenced by Bigdye Terminator Cycle Sequencing Ready Reaction kit, according to the manufacturer's protocols. Results: Two probable haplotypes that are associated with the DRB1*13:50:01 were observed among the 23,064 Taiwanese randomized unrelated individuals. One of the haplotypes is observed in 39 individuals while the other in two individuals. Conclusion: The findings in this study may be useful in studies reinforcing the understanding and clinical application of the polymorphism of HLA genes and haplotypes. |
| first_indexed | 2024-12-17T13:50:54Z |
| format | Article |
| id | doaj.art-a482ec3e7a9844249dccdc21b13cc62a |
| institution | Directory Open Access Journal |
| issn | 1016-3190 2223-8956 |
| language | English |
| last_indexed | 2024-12-17T13:50:54Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Tzu-Chi Medical Journal |
| spelling | doaj.art-a482ec3e7a9844249dccdc21b13cc62a2022-12-21T21:46:03ZengWolters Kluwer Medknow PublicationsTzu-Chi Medical Journal1016-31902223-89562021-01-0133437037310.4103/tcmj.tcmj_304_20Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individualsNondumiso Ayanda MazibukoKuo-Liang YangObjectives: Here, we show two probable haplotypes associated with the human leukocyte antigen (HLA) DRB1*13:50:01 allele. The haplotypes were observed from 41 randomized unrelated Taiwanese individuals among a population of 23,064 individuals tested. Materials and Methods: The samples in this study were blood samples, preserved in dipotassium ethylenediaminetetraacetic acid and/or ACD anticoagulants. The population is of donors from Tzu Chi Bone Marrow Donor Registry. Allele typing was performed using the sequence-based typing method, Sanger's sequencing. To discern the HLA-A and HLA-B alleles, exons 2 and 3 were sequenced. For DRB1 alleles, exon 2 was sequenced. Target exon sequence amplifications were done by a polymerase chain reaction and the resulting amplicons were sequenced by Bigdye Terminator Cycle Sequencing Ready Reaction kit, according to the manufacturer's protocols. Results: Two probable haplotypes that are associated with the DRB1*13:50:01 were observed among the 23,064 Taiwanese randomized unrelated individuals. One of the haplotypes is observed in 39 individuals while the other in two individuals. Conclusion: The findings in this study may be useful in studies reinforcing the understanding and clinical application of the polymorphism of HLA genes and haplotypes.http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2021;volume=33;issue=4;spage=370;epage=373;aulast=Mazibukodrb*13:50:01haplotypeshuman leukocyte antigentaiwanese |
| spellingShingle | Nondumiso Ayanda Mazibuko Kuo-Liang Yang Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals Tzu-Chi Medical Journal drb*13:50:01 haplotypes human leukocyte antigen taiwanese |
| title | Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals |
| title_full | Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals |
| title_fullStr | Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals |
| title_full_unstemmed | Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals |
| title_short | Two probable human leukocyte antigen haplotypes in association with human leukocyte antigen HLA-DRB1*13:50:01 identified in 41 randomized unrelated Taiwanese individuals |
| title_sort | two probable human leukocyte antigen haplotypes in association with human leukocyte antigen hla drb1 13 50 01 identified in 41 randomized unrelated taiwanese individuals |
| topic | drb*13:50:01 haplotypes human leukocyte antigen taiwanese |
| url | http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2021;volume=33;issue=4;spage=370;epage=373;aulast=Mazibuko |
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