Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital
Nosocomial spread of B. cepacia complex (Bcc) isolates amongst non-CF patients has been documented, where inadequate laboratory identification and limited treatment options are considered the main obstacles hindering accurate diagnosis and thus proper therapeutic outcome. The present study aimed to...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-03-01
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| Series: | Alexandria Journal of Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090506814000852 |
| _version_ | 1818452381969416192 |
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| author | Nancy Omar Hala Abd El Raouf Hadir Okasha Nermien Nabil |
| author_facet | Nancy Omar Hala Abd El Raouf Hadir Okasha Nermien Nabil |
| author_sort | Nancy Omar |
| collection | DOAJ |
| description | Nosocomial spread of B. cepacia complex (Bcc) isolates amongst non-CF patients has been documented, where inadequate laboratory identification and limited treatment options are considered the main obstacles hindering accurate diagnosis and thus proper therapeutic outcome.
The present study aimed to detect the isolation percentage of Bcc from patients in Alexandria Medical University Hospital (AMUH) according to site of infection (specimen), throughout a 6 month period. Out of 2079 specimens submitted to the microbiology laboratory, 35 strains were isolated on BCSA and biochemically identified as Bcc for the first time in this laboratory.
The highest rate of isolation of Bcc isolates was from pus (85.7%) isolated from patients in the burn unit. Antibiotic susceptibility tests revealed that all Bcc isolated were Multi Drug Resistant (MDR), the highest susceptibility was to meropenem (88.5%) followed by ceftazidime (60%), tobramycin, chloramphenicol, piperacillin–tazobactam and tetracycline, while all strains were resistant to co-trimoxazole and ciprofloxacin.
Minimal Inhibitory Concentration (MIC) determining tests showed that only 11.5% were resistant to meropenem at MIC > 16 μg/ml, while 40% of the strains were resistant to ceftazidime at MIC > 32 μg/ml. Those results for the time being indicate that meropenem is the best therapeutic option for Bcc infections in AMUH. |
| first_indexed | 2024-12-14T21:22:12Z |
| format | Article |
| id | doaj.art-a48374404af14ddd81daea9165be8774 |
| institution | Directory Open Access Journal |
| issn | 2090-5068 |
| language | English |
| last_indexed | 2024-12-14T21:22:12Z |
| publishDate | 2015-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Alexandria Journal of Medicine |
| spelling | doaj.art-a48374404af14ddd81daea9165be87742022-12-21T22:46:55ZengTaylor & Francis GroupAlexandria Journal of Medicine2090-50682015-03-01511414610.1016/j.ajme.2014.08.005Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University HospitalNancy Omar0Hala Abd El Raouf1Hadir Okasha2Nermien Nabil3Department of Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University, Alexandria, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University, Alexandria, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University, Alexandria, EgyptEl Gomhoreia Hospital, EgyptNosocomial spread of B. cepacia complex (Bcc) isolates amongst non-CF patients has been documented, where inadequate laboratory identification and limited treatment options are considered the main obstacles hindering accurate diagnosis and thus proper therapeutic outcome. The present study aimed to detect the isolation percentage of Bcc from patients in Alexandria Medical University Hospital (AMUH) according to site of infection (specimen), throughout a 6 month period. Out of 2079 specimens submitted to the microbiology laboratory, 35 strains were isolated on BCSA and biochemically identified as Bcc for the first time in this laboratory. The highest rate of isolation of Bcc isolates was from pus (85.7%) isolated from patients in the burn unit. Antibiotic susceptibility tests revealed that all Bcc isolated were Multi Drug Resistant (MDR), the highest susceptibility was to meropenem (88.5%) followed by ceftazidime (60%), tobramycin, chloramphenicol, piperacillin–tazobactam and tetracycline, while all strains were resistant to co-trimoxazole and ciprofloxacin. Minimal Inhibitory Concentration (MIC) determining tests showed that only 11.5% were resistant to meropenem at MIC > 16 μg/ml, while 40% of the strains were resistant to ceftazidime at MIC > 32 μg/ml. Those results for the time being indicate that meropenem is the best therapeutic option for Bcc infections in AMUH.http://www.sciencedirect.com/science/article/pii/S2090506814000852Burkholderia cepacia complexIdentificationAntimicrobial susceptibility |
| spellingShingle | Nancy Omar Hala Abd El Raouf Hadir Okasha Nermien Nabil Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital Alexandria Journal of Medicine Burkholderia cepacia complex Identification Antimicrobial susceptibility |
| title | Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital |
| title_full | Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital |
| title_fullStr | Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital |
| title_full_unstemmed | Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital |
| title_short | Microbiological assessment of Burkholderia cepacia complex (Bcc) isolates in Alexandria Main University Hospital |
| title_sort | microbiological assessment of burkholderia cepacia complex bcc isolates in alexandria main university hospital |
| topic | Burkholderia cepacia complex Identification Antimicrobial susceptibility |
| url | http://www.sciencedirect.com/science/article/pii/S2090506814000852 |
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