Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells
<p>Abstract</p> <p>Background</p> <p>Breast cancer and its metastatic progression is mainly directed by epithelial to mesenchymal transition (EMT), a phenomenon supported by specific transcription factors and miRNAs.</p> <p>Methods</p> <p>In orde...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | BMC Cancer |
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| Online Access: | http://www.biomedcentral.com/1471-2407/12/445 |
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| author | Lambertini Elisabetta Lolli Andrea Vezzali Federica Penolazzi Letizia Gambari Roberto Piva Roberta |
| author_facet | Lambertini Elisabetta Lolli Andrea Vezzali Federica Penolazzi Letizia Gambari Roberto Piva Roberta |
| author_sort | Lambertini Elisabetta |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Breast cancer and its metastatic progression is mainly directed by epithelial to mesenchymal transition (EMT), a phenomenon supported by specific transcription factors and miRNAs.</p> <p>Methods</p> <p>In order to investigate a possible correlation between Slug transcription factor and miR-221, we performed Slug gene silencing in MDA-MB-231 breast cancer cells and evaluated the expression of genes involved in supporting the breast cancer phenotype, using qRT-PCR and Western blot analysis. Chromatin immunoprecipitation and wound healing assays were employed to determine a functional link between these two molecules.</p> <p>Results</p> <p>We showed that Slug silencing significantly decreased the level of miR-221 and vimentin, reactivated Estrogen Receptor α and increased E-cadherin and TRPS1 expression. We demonstrated that miR-221 is a Slug target gene, and identified a specific region of miR-221 promoter that is transcriptionally active and binds the transcription factor Slug “in vivo”. In addition, we showed that in Slug-silenced cells, wich retained residual miR-221 (about 38%), cell migration was strongly inhibited. Cell migration was inhibited, but to a less degree, following complete knockdown of miR-221 expression by transfection with antagomiR-221.</p> <p>Conclusions</p> <p>We report for the first time evidence of a correlation between Slug transcription factor and miR-221 in breast cancer cells. These studies suggest that miR-221 expression is, in part, dependent on Slug in breast cancer cells, and that Slug plays a more important role than miR-221 in cell migration and invasion.</p> |
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| id | doaj.art-a486fb039c5e4470b8ca94b7a85ccf7f |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-21T06:03:48Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
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| series | BMC Cancer |
| spelling | doaj.art-a486fb039c5e4470b8ca94b7a85ccf7f2022-12-21T19:13:43ZengBMCBMC Cancer1471-24072012-10-0112144510.1186/1471-2407-12-445Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cellsLambertini ElisabettaLolli AndreaVezzali FedericaPenolazzi LetiziaGambari RobertoPiva Roberta<p>Abstract</p> <p>Background</p> <p>Breast cancer and its metastatic progression is mainly directed by epithelial to mesenchymal transition (EMT), a phenomenon supported by specific transcription factors and miRNAs.</p> <p>Methods</p> <p>In order to investigate a possible correlation between Slug transcription factor and miR-221, we performed Slug gene silencing in MDA-MB-231 breast cancer cells and evaluated the expression of genes involved in supporting the breast cancer phenotype, using qRT-PCR and Western blot analysis. Chromatin immunoprecipitation and wound healing assays were employed to determine a functional link between these two molecules.</p> <p>Results</p> <p>We showed that Slug silencing significantly decreased the level of miR-221 and vimentin, reactivated Estrogen Receptor α and increased E-cadherin and TRPS1 expression. We demonstrated that miR-221 is a Slug target gene, and identified a specific region of miR-221 promoter that is transcriptionally active and binds the transcription factor Slug “in vivo”. In addition, we showed that in Slug-silenced cells, wich retained residual miR-221 (about 38%), cell migration was strongly inhibited. Cell migration was inhibited, but to a less degree, following complete knockdown of miR-221 expression by transfection with antagomiR-221.</p> <p>Conclusions</p> <p>We report for the first time evidence of a correlation between Slug transcription factor and miR-221 in breast cancer cells. These studies suggest that miR-221 expression is, in part, dependent on Slug in breast cancer cells, and that Slug plays a more important role than miR-221 in cell migration and invasion.</p>http://www.biomedcentral.com/1471-2407/12/445SlugmiR-221Epithelial mesenchymal transitionBreast cancer |
| spellingShingle | Lambertini Elisabetta Lolli Andrea Vezzali Federica Penolazzi Letizia Gambari Roberto Piva Roberta Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells BMC Cancer Slug miR-221 Epithelial mesenchymal transition Breast cancer |
| title | Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells |
| title_full | Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells |
| title_fullStr | Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells |
| title_full_unstemmed | Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells |
| title_short | Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells |
| title_sort | correlation between slug transcription factor and mir 221 in mda mb 231 breast cancer cells |
| topic | Slug miR-221 Epithelial mesenchymal transition Breast cancer |
| url | http://www.biomedcentral.com/1471-2407/12/445 |
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