Transmissive multifocal laser speckle contrast imaging through thick tissue
Laser speckle contrast imaging (LSCI) is a powerful tool for monitoring blood flow changes in tissue or vessels in vivo, but its applications are limited by shallow penetration depth under reflective imaging configuration. The traditional LSCI setup has been used in transmissive imaging for depth ex...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2023-09-01
|
| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545823500050 |
| _version_ | 1797685185259503616 |
|---|---|
| author | Ruoyu Chen Peng Miao Shanbao Tong |
| author_facet | Ruoyu Chen Peng Miao Shanbao Tong |
| author_sort | Ruoyu Chen |
| collection | DOAJ |
| description | Laser speckle contrast imaging (LSCI) is a powerful tool for monitoring blood flow changes in tissue or vessels in vivo, but its applications are limited by shallow penetration depth under reflective imaging configuration. The traditional LSCI setup has been used in transmissive imaging for depth extension up to [Formula: see text]–[Formula: see text] ([Formula: see text] is the transport mean free path), but the blood flow estimation is biased due to the depth uncertainty in large depth of field (DOF) images. In this study, we propose a transmissive multifocal LSCI for depth-resolved blood flow in thick tissue, further extending the transmissive LSCI for tissue thickness up to [Formula: see text]. The limited-DOF imaging system is applied to the multifocal acquisition, and the depth of the vessel is estimated using a robust visibility parameter [Formula: see text] in the coherent domain. The accuracy and linearity of depth estimation are tested by Monte Carlo simulations. Based on the proposed method, the model of contrast analysis resolving the depth information is established and verified in a phantom experiment. We demonstrated its effectiveness in acquiring depth-resolved vessel structures and flow dynamics in in vivo imaging of chick embryos. |
| first_indexed | 2024-03-12T00:40:28Z |
| format | Article |
| id | doaj.art-a4897c7063b4468fa717f887cb16a93b |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-03-12T00:40:28Z |
| publishDate | 2023-09-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-a4897c7063b4468fa717f887cb16a93b2023-09-15T08:16:34ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-09-01160510.1142/S1793545823500050Transmissive multifocal laser speckle contrast imaging through thick tissueRuoyu Chen0Peng Miao1Shanbao Tong2School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, P. R. ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, P. R. ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, P. R. ChinaLaser speckle contrast imaging (LSCI) is a powerful tool for monitoring blood flow changes in tissue or vessels in vivo, but its applications are limited by shallow penetration depth under reflective imaging configuration. The traditional LSCI setup has been used in transmissive imaging for depth extension up to [Formula: see text]–[Formula: see text] ([Formula: see text] is the transport mean free path), but the blood flow estimation is biased due to the depth uncertainty in large depth of field (DOF) images. In this study, we propose a transmissive multifocal LSCI for depth-resolved blood flow in thick tissue, further extending the transmissive LSCI for tissue thickness up to [Formula: see text]. The limited-DOF imaging system is applied to the multifocal acquisition, and the depth of the vessel is estimated using a robust visibility parameter [Formula: see text] in the coherent domain. The accuracy and linearity of depth estimation are tested by Monte Carlo simulations. Based on the proposed method, the model of contrast analysis resolving the depth information is established and verified in a phantom experiment. We demonstrated its effectiveness in acquiring depth-resolved vessel structures and flow dynamics in in vivo imaging of chick embryos.https://www.worldscientific.com/doi/10.1142/S1793545823500050Transmissive imagingmultifocal imagingdepthlaser speckle contrast model |
| spellingShingle | Ruoyu Chen Peng Miao Shanbao Tong Transmissive multifocal laser speckle contrast imaging through thick tissue Journal of Innovative Optical Health Sciences Transmissive imaging multifocal imaging depth laser speckle contrast model |
| title | Transmissive multifocal laser speckle contrast imaging through thick tissue |
| title_full | Transmissive multifocal laser speckle contrast imaging through thick tissue |
| title_fullStr | Transmissive multifocal laser speckle contrast imaging through thick tissue |
| title_full_unstemmed | Transmissive multifocal laser speckle contrast imaging through thick tissue |
| title_short | Transmissive multifocal laser speckle contrast imaging through thick tissue |
| title_sort | transmissive multifocal laser speckle contrast imaging through thick tissue |
| topic | Transmissive imaging multifocal imaging depth laser speckle contrast model |
| url | https://www.worldscientific.com/doi/10.1142/S1793545823500050 |
| work_keys_str_mv | AT ruoyuchen transmissivemultifocallaserspecklecontrastimagingthroughthicktissue AT pengmiao transmissivemultifocallaserspecklecontrastimagingthroughthicktissue AT shanbaotong transmissivemultifocallaserspecklecontrastimagingthroughthicktissue |