Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses
Background: Barley yellow dwarf in wheat is an important viral disease among wheat cultivating areas of the world. It is gradually progressing as a major threat to wheat crop in Pakistan due to availability of favorable environmental conditions. The use of resistant cultivar is environmentally safes...
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| Format: | Article |
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The Running Line
2015-11-01
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| Series: | Advancements in Life Sciences |
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| Online Access: | http://www.als-journal.com/313-15/ |
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| author | Samra Kausar Shahid Hameed Irfan ul Haque Kamran Saleem Madiha Zamurrad Muhammad Ashfaq |
| author_facet | Samra Kausar Shahid Hameed Irfan ul Haque Kamran Saleem Madiha Zamurrad Muhammad Ashfaq |
| author_sort | Samra Kausar |
| collection | DOAJ |
| description | Background: Barley yellow dwarf in wheat is an important viral disease among wheat cultivating areas of the world. It is gradually progressing as a major threat to wheat crop in Pakistan due to availability of favorable environmental conditions. The use of resistant cultivar is environmentally safest method for disease control so, it is necessary to develop resistant cultivars before epidemic outbreaks.
Methods: The most commonly used wheat variety Inqilab91 was crossed with BYDV / CYDV resistant variety TC14. F1 generation obtained from the P1 cross was then allowed to self-cross. 61 plants were selected from F2 generation on the basis of disease tolerance or susceptibility and only tolerant plants were included for further experiments in the study. The presence of BDV2 among F2 generation was confirmed by sequence characterized amplified region (SCAR) markers in 6 (91, 96, 110, 119, 121 and 131) out of 61 genotypes which were then backcrossed with recurrent parent. Advance analysis regarding the presence of resistance source among the selected F2 generation was carried out using ELISA. Moreover, appearance of symptoms, agronomic values for different parameters, green house and field responses were also kept under consideration to characterize and confirm the presence of BDV2 among plants.
Results: Results indicated that majority of F2 segregating population showed less yellowing, low viral titer and good agronomic values. ELISA value, glasshouse and field analysis showed that seven genotypes (30, 81, 89, 91,101,110 and 121) were resistant, and twenty-four genotypes were found moderately resistant. Tolerance was detected in genotypes 31, 47, 48, 50, 52, 56, 57, 60, 61, 94, 103, 106, 113, 115, 127, 140 and 413.
Conclusion: Wheat lines containing Bdv2 genes showed resistance in both field and glasshouse. These wheat germplasm could be used as a source of resistance in CDRP-NARC for the further development of resistant wheat varieties against BYDV / CYDV. |
| first_indexed | 2024-03-12T18:45:52Z |
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| issn | 2310-5380 2310-5380 |
| language | English |
| last_indexed | 2024-03-12T18:45:52Z |
| publishDate | 2015-11-01 |
| publisher | The Running Line |
| record_format | Article |
| series | Advancements in Life Sciences |
| spelling | doaj.art-a48b429586154ffc928aa7bce63b6dca2023-08-02T07:41:02ZengThe Running LineAdvancements in Life Sciences2310-53802310-53802015-11-01311622Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf virusesSamra Kausar0Shahid Hameed1Irfan ul Haque2Kamran Saleem3Madiha Zamurrad4Muhammad Ashfaq5Adaptive Research Farm, Chakwal – PakistanCDRI, NARC Islamabad – PakistanPMAS-Arid Agriculture University Rawalpindi – PakistanNuclear Institute for Agriculture and Biology (NIAB) Faisalabad – Pakistan PMAS-Arid Agriculture University Rawalpindi – PakistanPMAS-Arid Agriculture University Rawalpindi – PakistanBackground: Barley yellow dwarf in wheat is an important viral disease among wheat cultivating areas of the world. It is gradually progressing as a major threat to wheat crop in Pakistan due to availability of favorable environmental conditions. The use of resistant cultivar is environmentally safest method for disease control so, it is necessary to develop resistant cultivars before epidemic outbreaks. Methods: The most commonly used wheat variety Inqilab91 was crossed with BYDV / CYDV resistant variety TC14. F1 generation obtained from the P1 cross was then allowed to self-cross. 61 plants were selected from F2 generation on the basis of disease tolerance or susceptibility and only tolerant plants were included for further experiments in the study. The presence of BDV2 among F2 generation was confirmed by sequence characterized amplified region (SCAR) markers in 6 (91, 96, 110, 119, 121 and 131) out of 61 genotypes which were then backcrossed with recurrent parent. Advance analysis regarding the presence of resistance source among the selected F2 generation was carried out using ELISA. Moreover, appearance of symptoms, agronomic values for different parameters, green house and field responses were also kept under consideration to characterize and confirm the presence of BDV2 among plants. Results: Results indicated that majority of F2 segregating population showed less yellowing, low viral titer and good agronomic values. ELISA value, glasshouse and field analysis showed that seven genotypes (30, 81, 89, 91,101,110 and 121) were resistant, and twenty-four genotypes were found moderately resistant. Tolerance was detected in genotypes 31, 47, 48, 50, 52, 56, 57, 60, 61, 94, 103, 106, 113, 115, 127, 140 and 413. Conclusion: Wheat lines containing Bdv2 genes showed resistance in both field and glasshouse. These wheat germplasm could be used as a source of resistance in CDRP-NARC for the further development of resistant wheat varieties against BYDV / CYDV.http://www.als-journal.com/313-15/VirusEpidemicresistant geneELISAMarker |
| spellingShingle | Samra Kausar Shahid Hameed Irfan ul Haque Kamran Saleem Madiha Zamurrad Muhammad Ashfaq Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses Advancements in Life Sciences Virus Epidemic resistant gene ELISA Marker |
| title | Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses |
| title_full | Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses |
| title_fullStr | Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses |
| title_full_unstemmed | Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses |
| title_short | Molecular confirmation of Bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses |
| title_sort | molecular confirmation of bdv2 gene in wheat germplasm and its field based assessment for resistance against barely yellow dwarf viruses |
| topic | Virus Epidemic resistant gene ELISA Marker |
| url | http://www.als-journal.com/313-15/ |
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