Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia
Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2022-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/effect-of-glucose-on-endo-xylanase-and-%ce%b2-xylosidase-production-by-fungi-isolated-in-indonesia/ |
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| author | Ririn Krisnawati Sardjono Jaka Widada Dian Anggraini Suroto Muhammad Nur Cahyanto |
| author_facet | Ririn Krisnawati Sardjono Jaka Widada Dian Anggraini Suroto Muhammad Nur Cahyanto |
| author_sort | Ririn Krisnawati |
| collection | DOAJ |
| description | Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endo-xylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021–0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined. |
| first_indexed | 2024-12-18T05:22:58Z |
| format | Article |
| id | doaj.art-a48be558fa1040739668821e87494bf7 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-18T05:22:58Z |
| publishDate | 2022-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-a48be558fa1040739668821e87494bf72022-12-21T21:19:36ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-03-01161226234https://doi.org/10.22207/JPAM.16.1.12Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in IndonesiaRirin Krisnawatihttps://orcid.org/0000-0001-5851-060XSardjonoJaka Widadahttps://orcid.org/0000-0001-5012-3795Dian Anggraini Surotohttps://orcid.org/0000-0002-4438-1752Muhammad Nur Cahyantohttps://orcid.org/0000-0002-1448-876XXylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endo-xylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021–0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined.https://microbiologyjournal.org/effect-of-glucose-on-endo-xylanase-and-%ce%b2-xylosidase-production-by-fungi-isolated-in-indonesia/endo-xylanaseβ-xylosidasecarbon catabolite repressionglucose |
| spellingShingle | Ririn Krisnawati Sardjono Jaka Widada Dian Anggraini Suroto Muhammad Nur Cahyanto Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia Journal of Pure and Applied Microbiology endo-xylanase β-xylosidase carbon catabolite repression glucose |
| title | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_full | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_fullStr | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_full_unstemmed | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_short | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_sort | effect of glucose on endo xylanase and β xylosidase production by fungi isolated in indonesia |
| topic | endo-xylanase β-xylosidase carbon catabolite repression glucose |
| url | https://microbiologyjournal.org/effect-of-glucose-on-endo-xylanase-and-%ce%b2-xylosidase-production-by-fungi-isolated-in-indonesia/ |
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