Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study
Bone-like viable tissue can be generated in vitro by utilizing a combination of inorganic matrix, osteoblasts, osteogenic media and application of adequate mechanical stimulation of the cells. To pursue the proof that the in vitro generated bone-like tissue (BLT) is capable of bridging a critical bo...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-01-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020300360 |
| _version_ | 1818778455912742912 |
|---|---|
| author | Nahum Rosenberg Orit Rosenberg |
| author_facet | Nahum Rosenberg Orit Rosenberg |
| author_sort | Nahum Rosenberg |
| collection | DOAJ |
| description | Bone-like viable tissue can be generated in vitro by utilizing a combination of inorganic matrix, osteoblasts, osteogenic media and application of adequate mechanical stimulation of the cells. To pursue the proof that the in vitro generated bone-like tissue (BLT) is capable of bridging a critical bone gap in vivo without adverse effects, the in vitro cytotoxicity method (MTT) and murine in vivo model were implemented, by implanting the BLT into calvaria critical bone gap in rats. The endpoints for the evaluation of this concept were histological and radiographic data which should show the effectiveness of this method. We found that there was no cytotoxic effect of the BLT according to the MTT assay and no carcinogenic or other morbid effects of the BLT in vivo (mice experiment, n = 10) The critical gaps in BLT -implanted animals (experimental model with rats) demonstrated full bridging of the calvaria critical bone gap with vascularized woven bone (n = 3) as opposed to animals treated with vehicle material (n = 3), which maintained an open gap without any visible closure, according to gross examination, X-ray imaging and histological analysis. The newly formed bone tissue was characterized by pronounced presence of bone marrow regions and newly formed host blood vessels, a strong indication for functional osseointegration. Therefore, the in vitro generated BLT, which causes bone regeneration in critical gaps, has the translational potential to bridge bone non-union defects, without harmful systemic or cytotoxic effects. These initial feasibility results indicate a high safety profile following in vivo implantation of BLT and its potential clinical ability to be used as autologous bone graft. |
| first_indexed | 2024-12-18T11:45:00Z |
| format | Article |
| id | doaj.art-a48c53b562aa4322850517b48447b38e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-18T11:45:00Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-a48c53b562aa4322850517b48447b38e2022-12-21T21:09:18ZengElsevierHeliyon2405-84402020-01-0161e03191Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility studyNahum Rosenberg0Orit Rosenberg1Faculty of Medicine, Technion – IIT, Israel; I.M. Sechenov First Moscow State Medical University, Moscow, Russia; Corresponding author.Sheltagen Medical Ltd, Haifa, IsraelBone-like viable tissue can be generated in vitro by utilizing a combination of inorganic matrix, osteoblasts, osteogenic media and application of adequate mechanical stimulation of the cells. To pursue the proof that the in vitro generated bone-like tissue (BLT) is capable of bridging a critical bone gap in vivo without adverse effects, the in vitro cytotoxicity method (MTT) and murine in vivo model were implemented, by implanting the BLT into calvaria critical bone gap in rats. The endpoints for the evaluation of this concept were histological and radiographic data which should show the effectiveness of this method. We found that there was no cytotoxic effect of the BLT according to the MTT assay and no carcinogenic or other morbid effects of the BLT in vivo (mice experiment, n = 10) The critical gaps in BLT -implanted animals (experimental model with rats) demonstrated full bridging of the calvaria critical bone gap with vascularized woven bone (n = 3) as opposed to animals treated with vehicle material (n = 3), which maintained an open gap without any visible closure, according to gross examination, X-ray imaging and histological analysis. The newly formed bone tissue was characterized by pronounced presence of bone marrow regions and newly formed host blood vessels, a strong indication for functional osseointegration. Therefore, the in vitro generated BLT, which causes bone regeneration in critical gaps, has the translational potential to bridge bone non-union defects, without harmful systemic or cytotoxic effects. These initial feasibility results indicate a high safety profile following in vivo implantation of BLT and its potential clinical ability to be used as autologous bone graft.http://www.sciencedirect.com/science/article/pii/S2405844020300360Biomedical engineeringBiotechnologyBioengineeringBonePathophysiologyRegenerative medicine |
| spellingShingle | Nahum Rosenberg Orit Rosenberg Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study Heliyon Biomedical engineering Biotechnology Bioengineering Bone Pathophysiology Regenerative medicine |
| title | Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study |
| title_full | Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study |
| title_fullStr | Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study |
| title_full_unstemmed | Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study |
| title_short | Safety and efficacy of in vitro generated bone-like material for in vivo bone regeneration – a feasibility study |
| title_sort | safety and efficacy of in vitro generated bone like material for in vivo bone regeneration a feasibility study |
| topic | Biomedical engineering Biotechnology Bioengineering Bone Pathophysiology Regenerative medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020300360 |
| work_keys_str_mv | AT nahumrosenberg safetyandefficacyofinvitrogeneratedbonelikematerialforinvivoboneregenerationafeasibilitystudy AT oritrosenberg safetyandefficacyofinvitrogeneratedbonelikematerialforinvivoboneregenerationafeasibilitystudy |