Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline
Haspin is a histone kinase that promotes error-free chromosome segregation by recruiting the chromosomal passenger complex (CPC) to mitotic and meiotic chromosomes. Haspin remains less well studied than other M-phase kinases, and the models explaining Haspin function have been developed primarily in...
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| Format: | Article |
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The Company of Biologists
2022-07-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/11/7/bio059277 |
| _version_ | 1828375207801257984 |
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| author | Jommel Macaraeg Isaac Reinhard Matthew Ward Danielle Carmeci Madison Stanaway Amy Moore Ethan Hagmann Katherine Brown David J. Wynne |
| author_facet | Jommel Macaraeg Isaac Reinhard Matthew Ward Danielle Carmeci Madison Stanaway Amy Moore Ethan Hagmann Katherine Brown David J. Wynne |
| author_sort | Jommel Macaraeg |
| collection | DOAJ |
| description | Haspin is a histone kinase that promotes error-free chromosome segregation by recruiting the chromosomal passenger complex (CPC) to mitotic and meiotic chromosomes. Haspin remains less well studied than other M-phase kinases, and the models explaining Haspin function have been developed primarily in mitotic cells. Here, we generate strains containing new conditional or nonsense mutations in the Caenorhabditis elegans Haspin homologs hasp-1 and hasp-2 and characterize their phenotypes. We show that hasp-1 is responsible for all predicted functions of Haspin and that loss of function of hasp-1 using classical and conditional alleles produces defects in germline stem cell proliferation and spermatogenesis, and confirms its role in oocyte meiosis. Genetic analysis suggests that hasp-1 acts downstream of the Polo-like kinase plk-2 and shows synthetic interactions between hasp-1 and two genes expected to promote recruitment of the CPC by a parallel pathway that depends on the kinase Bub1. This work adds to the growing understanding of Haspin function by characterizing a variety of roles in an intact animal. |
| first_indexed | 2024-04-14T07:43:35Z |
| format | Article |
| id | doaj.art-a48db24ec5194adfb8a90537f5e50b22 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-04-14T07:43:35Z |
| publishDate | 2022-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-a48db24ec5194adfb8a90537f5e50b222022-12-22T02:05:25ZengThe Company of BiologistsBiology Open2046-63902022-07-0111710.1242/bio.059277059277Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germlineJommel Macaraeg0Isaac Reinhard1Matthew Ward2Danielle Carmeci3Madison Stanaway4Amy Moore5Ethan Hagmann6Katherine Brown7David J. Wynne8 Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Department of Biology, University of Portland, 5000 N Willamette Blvd, Portland, OR 97203, USA Haspin is a histone kinase that promotes error-free chromosome segregation by recruiting the chromosomal passenger complex (CPC) to mitotic and meiotic chromosomes. Haspin remains less well studied than other M-phase kinases, and the models explaining Haspin function have been developed primarily in mitotic cells. Here, we generate strains containing new conditional or nonsense mutations in the Caenorhabditis elegans Haspin homologs hasp-1 and hasp-2 and characterize their phenotypes. We show that hasp-1 is responsible for all predicted functions of Haspin and that loss of function of hasp-1 using classical and conditional alleles produces defects in germline stem cell proliferation and spermatogenesis, and confirms its role in oocyte meiosis. Genetic analysis suggests that hasp-1 acts downstream of the Polo-like kinase plk-2 and shows synthetic interactions between hasp-1 and two genes expected to promote recruitment of the CPC by a parallel pathway that depends on the kinase Bub1. This work adds to the growing understanding of Haspin function by characterizing a variety of roles in an intact animal.http://bio.biologists.org/content/11/7/bio059277chromosome segregationinner centromeregermlinemeiosisspermatogenesisoogenesis |
| spellingShingle | Jommel Macaraeg Isaac Reinhard Matthew Ward Danielle Carmeci Madison Stanaway Amy Moore Ethan Hagmann Katherine Brown David J. Wynne Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline Biology Open chromosome segregation inner centromere germline meiosis spermatogenesis oogenesis |
| title | Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline |
| title_full | Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline |
| title_fullStr | Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline |
| title_full_unstemmed | Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline |
| title_short | Genetic analysis of Caenorhabditis elegans Haspin-like genes shows that hasp-1 plays multiple roles in the germline |
| title_sort | genetic analysis of caenorhabditis elegans haspin like genes shows that hasp 1 plays multiple roles in the germline |
| topic | chromosome segregation inner centromere germline meiosis spermatogenesis oogenesis |
| url | http://bio.biologists.org/content/11/7/bio059277 |
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