Activation of pRL-TK by 12S E1A Oncoprotein: Drawbacks of Using an Internal Reference Reporter in Transcription Assays
In transient expression assays for transcription, a vector carrying the experimental reporter is usually co-transfected with a second vector containing a distinct reporter gene as a control. The second reporter is linked to a constitutive promoter driving a low-level transcription that is unresponsi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01313st06 |