Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale
The bioavailability of carotenoids from kale was investigated by labeling nutrients in kale with 13C, feeding the kale to seven adult volunteers, and analyzing serial plasma samples for labeled lutein, β-carotene, and retinol. Ingested doses of labeled carotenoids were 34 μmol for β-carotene and 33...
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| Format: | Article |
| Language: | English |
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Elsevier
2005-09-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520329369 |
| _version_ | 1818385423731261440 |
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| author | Janet A. Novotny Anne C. Kurilich Steven J. Britz Beverly A. Clevidence |
| author_facet | Janet A. Novotny Anne C. Kurilich Steven J. Britz Beverly A. Clevidence |
| author_sort | Janet A. Novotny |
| collection | DOAJ |
| description | The bioavailability of carotenoids from kale was investigated by labeling nutrients in kale with 13C, feeding the kale to seven adult volunteers, and analyzing serial plasma samples for labeled lutein, β-carotene, and retinol. Ingested doses of labeled carotenoids were 34 μmol for β-carotene and 33 μmol for lutein. Peak plasma concentrations, areas under the plasma concentration-time curves (AUCs), and percentages of dose recovered at peak plasma concentrations were calculated. Average peak plasma concentrations were 0.38, 0.068, and 0.079 μM for [13C]lutein, [13C]β-carotene, and [13C]retinol, respectively. Average AUC values (over 28 days) were 42.8, 13.6, 13.2 μM h for [13C]lutein, [13C]β-carotene, and [13C]retinol, respectively. Percentages of dose recovered at peak plasma concentrations were 3.6, 0.7, and 0.7% for [13C]lutein, [13C]β-carotene, and [13C]retinol, respectively. A positive relationship was observed between baseline plasma retinol levels and [13C]retinol plasma response.It is possible that this relationship was mediated either through some aspect of β-carotene absorption or via the common pathways of metabolism for postdose and endogenous retinoid. |
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| id | doaj.art-a48fe1d3b8b24ba3952bd4d7665dac20 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:37:55Z |
| publishDate | 2005-09-01 |
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| series | Journal of Lipid Research |
| spelling | doaj.art-a48fe1d3b8b24ba3952bd4d7665dac202022-12-21T23:18:34ZengElsevierJournal of Lipid Research0022-22752005-09-0146918961903Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kaleJanet A. Novotny0Anne C. Kurilich1Steven J. Britz2Beverly A. Clevidence3To whom correspondence should be addressed.; United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705The bioavailability of carotenoids from kale was investigated by labeling nutrients in kale with 13C, feeding the kale to seven adult volunteers, and analyzing serial plasma samples for labeled lutein, β-carotene, and retinol. Ingested doses of labeled carotenoids were 34 μmol for β-carotene and 33 μmol for lutein. Peak plasma concentrations, areas under the plasma concentration-time curves (AUCs), and percentages of dose recovered at peak plasma concentrations were calculated. Average peak plasma concentrations were 0.38, 0.068, and 0.079 μM for [13C]lutein, [13C]β-carotene, and [13C]retinol, respectively. Average AUC values (over 28 days) were 42.8, 13.6, 13.2 μM h for [13C]lutein, [13C]β-carotene, and [13C]retinol, respectively. Percentages of dose recovered at peak plasma concentrations were 3.6, 0.7, and 0.7% for [13C]lutein, [13C]β-carotene, and [13C]retinol, respectively. A positive relationship was observed between baseline plasma retinol levels and [13C]retinol plasma response.It is possible that this relationship was mediated either through some aspect of β-carotene absorption or via the common pathways of metabolism for postdose and endogenous retinoid.http://www.sciencedirect.com/science/article/pii/S0022227520329369bioavailabilityvitamin Aliquid chromatography-mass spectrometryBrassica oleracea |
| spellingShingle | Janet A. Novotny Anne C. Kurilich Steven J. Britz Beverly A. Clevidence Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale Journal of Lipid Research bioavailability vitamin A liquid chromatography-mass spectrometry Brassica oleracea |
| title | Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale |
| title_full | Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale |
| title_fullStr | Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale |
| title_full_unstemmed | Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale |
| title_short | Plasma appearance of labeled β-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale |
| title_sort | plasma appearance of labeled β carotene lutein and retinol in humans after consumption of isotopically labeled kale |
| topic | bioavailability vitamin A liquid chromatography-mass spectrometry Brassica oleracea |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520329369 |
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