Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights
Glycerol is commonly employed for denitrification purposes in full-scale wastewater treatment. In non-acclimatized biomass, the glycerol is very inefficient resulting in a high C/N ratio and low-standard denitrification rates. The acclimatization is driven by the microbial enrichment of Saccharimona...
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| Format: | Article |
| Language: | English |
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IWA Publishing
2023-08-01
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| Series: | Water Science and Technology |
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| Online Access: | http://wst.iwaponline.com/content/88/3/645 |
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| author | Fenu Alessio Dockx Lennert Van Damme Sandra Wynants Frederik Kirke Verhaeghe Van Laer Koen Roels Joris Delgado Rosalia |
| author_facet | Fenu Alessio Dockx Lennert Van Damme Sandra Wynants Frederik Kirke Verhaeghe Van Laer Koen Roels Joris Delgado Rosalia |
| author_sort | Fenu Alessio |
| collection | DOAJ |
| description | Glycerol is commonly employed for denitrification purposes in full-scale wastewater treatment. In non-acclimatized biomass, the glycerol is very inefficient resulting in a high C/N ratio and low-standard denitrification rates. The acclimatization is driven by the microbial enrichment of Saccharimonadales and Propionibacteriales as found in different sampled municipal sludges flanking the dominant presence of Burkholderiales. The selective strategy is based on a very efficient process in terms of C/N ratios and standard denitrification rates, but it leads to nitrite accumulation. As a result, severe and unexpected nitrous oxide emissions were found in full-scale with emission factors up to 2.5% kgN2O (kgKJNremoved)−1. Simultaneous dosage of isobutirate in a full-scale experiment could counter the nitrous oxide emissions. As nitrous oxide emissions were found proportional to the dosed glycerol-based COD, the authors suggest that, in case of acclimatization of biomass to glycerol, an emission factor based on the dosed COD should substitute the general nitrous oxide emission factors based on incoming or removed nitrogen to the plant.
HIGHLIGHTS
Acclimatization to glycerol leads to an increase in C/N ratios and standard denitrification rates.;
We observed microbial enrichment of Saccharimonadales and Propionibacteriales and nitrite accumulation.;
N2O emission factors reached up to 2.5% kgN2O/kgKJNremoved.;
In case of acclimatization, the N2O emission factor is best expressed in function of the dosed glycerol COD.;
Simultaneous isobutirate dosage countered the N2O emissions.; |
| first_indexed | 2024-03-12T01:45:39Z |
| format | Article |
| id | doaj.art-a491dd8fd55e4327835e13338e936507 |
| institution | Directory Open Access Journal |
| issn | 0273-1223 1996-9732 |
| language | English |
| last_indexed | 2024-03-12T01:45:39Z |
| publishDate | 2023-08-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Science and Technology |
| spelling | doaj.art-a491dd8fd55e4327835e13338e9365072023-09-09T08:58:49ZengIWA PublishingWater Science and Technology0273-12231996-97322023-08-0188364565710.2166/wst.2023.240240Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insightsFenu Alessio0Dockx Lennert1Van Damme Sandra2Wynants Frederik3Kirke Verhaeghe4Van Laer Koen5Roels Joris6Delgado Rosalia7 Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Aquafin NV, Dijkstraat 8, Aartselaar, Belgium Glycerol is commonly employed for denitrification purposes in full-scale wastewater treatment. In non-acclimatized biomass, the glycerol is very inefficient resulting in a high C/N ratio and low-standard denitrification rates. The acclimatization is driven by the microbial enrichment of Saccharimonadales and Propionibacteriales as found in different sampled municipal sludges flanking the dominant presence of Burkholderiales. The selective strategy is based on a very efficient process in terms of C/N ratios and standard denitrification rates, but it leads to nitrite accumulation. As a result, severe and unexpected nitrous oxide emissions were found in full-scale with emission factors up to 2.5% kgN2O (kgKJNremoved)−1. Simultaneous dosage of isobutirate in a full-scale experiment could counter the nitrous oxide emissions. As nitrous oxide emissions were found proportional to the dosed glycerol-based COD, the authors suggest that, in case of acclimatization of biomass to glycerol, an emission factor based on the dosed COD should substitute the general nitrous oxide emission factors based on incoming or removed nitrogen to the plant. HIGHLIGHTS Acclimatization to glycerol leads to an increase in C/N ratios and standard denitrification rates.; We observed microbial enrichment of Saccharimonadales and Propionibacteriales and nitrite accumulation.; N2O emission factors reached up to 2.5% kgN2O/kgKJNremoved.; In case of acclimatization, the N2O emission factor is best expressed in function of the dosed glycerol COD.; Simultaneous isobutirate dosage countered the N2O emissions.;http://wst.iwaponline.com/content/88/3/645glycerolnitrous oxidepartial denitrification16s rrna gene amplicon sequencing |
| spellingShingle | Fenu Alessio Dockx Lennert Van Damme Sandra Wynants Frederik Kirke Verhaeghe Van Laer Koen Roels Joris Delgado Rosalia Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights Water Science and Technology glycerol nitrous oxide partial denitrification 16s rrna gene amplicon sequencing |
| title | Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights |
| title_full | Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights |
| title_fullStr | Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights |
| title_full_unstemmed | Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights |
| title_short | Glycerol used for denitrification in full-scale wastewater treatment plants: nitrous oxide emissions, sludge acclimatization, and other insights |
| title_sort | glycerol used for denitrification in full scale wastewater treatment plants nitrous oxide emissions sludge acclimatization and other insights |
| topic | glycerol nitrous oxide partial denitrification 16s rrna gene amplicon sequencing |
| url | http://wst.iwaponline.com/content/88/3/645 |
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