Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele
Background: Dombrock blood group system genotyping has revealed various rearrangements of the Dombrock gene and identified new variant alleles in Brazil (i.e., DO*A-SH, DO*A-WL and DO*B-WL). Because of the high heterogeneity of the Brazilian population, interregional differences are expected during...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-01-01
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| Series: | Revista Brasileira de Hematologia e Hemoterapia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842013000600400&lng=en&tlng=en |
| _version_ | 1819238127425814528 |
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| author | Fabiana Chagas Camargos Piassi Silvana Maria Eloi Santos Lilian Maria de Castilho Wilson Baleotti Junior Rodrigo Buzinaro Suzuki Debora Moura da Cunha |
| author_facet | Fabiana Chagas Camargos Piassi Silvana Maria Eloi Santos Lilian Maria de Castilho Wilson Baleotti Junior Rodrigo Buzinaro Suzuki Debora Moura da Cunha |
| author_sort | Fabiana Chagas Camargos Piassi |
| collection | DOAJ |
| description | Background: Dombrock blood group system genotyping has revealed various rearrangements of the Dombrock gene and identified new variant alleles in Brazil (i.e., DO*A-SH, DO*A-WL and DO*B-WL). Because of the high heterogeneity of the Brazilian population, interregional differences are expected during the investigation of Dombrock genotypes. Objective: The present study aims to determine the frequencies of Dombrock genotypes in blood donors from Minas Gerais and compare the frequencies of the HY and JO alleles to those of another population in Brazil. Methods: The frequencies of the DO alleles in Minas Gerais, a southeastern state of Brazil, were determined from the genotyping of 270 blood donors. Genotyping involved polymerase chain reaction and restriction fragment length polymorphism analysis to identify the 323G>T, 350C>T, 793A>G, and 898C>G mutations, which are related to the HY, JO, DO*A/DO*B, and DO*A-WL/DO*B-WL alleles, respectively. Moreover, the frequencies of rare HY and JO alleles were statistically compared using the chi-square test with data from another Brazilian region. Results: The HY allele frequency in Minas Gerais (2.4%) was almost twice that of the JO allele (1.5%). The frequency of the HY allele was significantly higher (p-value = 0.001) than that in another Brazilian population and includes a rare homozygous donor with the Hy- phenotype. In addition, the DO*A-WL and DO*B-WL alleles, which were first identified in Brazil, were found in the state of Minas Gerais. Conclusions: The data confirm that the frequencies of DO alleles differ between regions in Brazil. The population of Minas Gerais could be targeted in a screening strategy to identify the Hy- phenotype in order to develop a rare blood bank. |
| first_indexed | 2024-12-23T13:31:17Z |
| format | Article |
| id | doaj.art-a4922aec923c45289168c20954b9bfc6 |
| institution | Directory Open Access Journal |
| issn | 1806-0870 |
| language | English |
| last_indexed | 2024-12-23T13:31:17Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Revista Brasileira de Hematologia e Hemoterapia |
| spelling | doaj.art-a4922aec923c45289168c20954b9bfc62022-12-21T17:45:10ZengElsevierRevista Brasileira de Hematologia e Hemoterapia1806-08702013-01-0135640040310.5581/1516-8484.20130128S1516-84842013000600400Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY alleleFabiana Chagas Camargos PiassiSilvana Maria Eloi SantosLilian Maria de CastilhoWilson Baleotti JuniorRodrigo Buzinaro SuzukiDebora Moura da CunhaBackground: Dombrock blood group system genotyping has revealed various rearrangements of the Dombrock gene and identified new variant alleles in Brazil (i.e., DO*A-SH, DO*A-WL and DO*B-WL). Because of the high heterogeneity of the Brazilian population, interregional differences are expected during the investigation of Dombrock genotypes. Objective: The present study aims to determine the frequencies of Dombrock genotypes in blood donors from Minas Gerais and compare the frequencies of the HY and JO alleles to those of another population in Brazil. Methods: The frequencies of the DO alleles in Minas Gerais, a southeastern state of Brazil, were determined from the genotyping of 270 blood donors. Genotyping involved polymerase chain reaction and restriction fragment length polymorphism analysis to identify the 323G>T, 350C>T, 793A>G, and 898C>G mutations, which are related to the HY, JO, DO*A/DO*B, and DO*A-WL/DO*B-WL alleles, respectively. Moreover, the frequencies of rare HY and JO alleles were statistically compared using the chi-square test with data from another Brazilian region. Results: The HY allele frequency in Minas Gerais (2.4%) was almost twice that of the JO allele (1.5%). The frequency of the HY allele was significantly higher (p-value = 0.001) than that in another Brazilian population and includes a rare homozygous donor with the Hy- phenotype. In addition, the DO*A-WL and DO*B-WL alleles, which were first identified in Brazil, were found in the state of Minas Gerais. Conclusions: The data confirm that the frequencies of DO alleles differ between regions in Brazil. The population of Minas Gerais could be targeted in a screening strategy to identify the Hy- phenotype in order to develop a rare blood bank.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842013000600400&lng=en&tlng=enBlood donorsPolymerase chain reactionGenotyping techniquesBlood group antigensPhenotypeH-Y antigen/bloodPolymorphism, restriction fragment lengthAllelesBrazil |
| spellingShingle | Fabiana Chagas Camargos Piassi Silvana Maria Eloi Santos Lilian Maria de Castilho Wilson Baleotti Junior Rodrigo Buzinaro Suzuki Debora Moura da Cunha Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele Revista Brasileira de Hematologia e Hemoterapia Blood donors Polymerase chain reaction Genotyping techniques Blood group antigens Phenotype H-Y antigen/blood Polymorphism, restriction fragment length Alleles Brazil |
| title | Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele |
| title_full | Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele |
| title_fullStr | Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele |
| title_full_unstemmed | Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele |
| title_short | Dombrock genotyping in Brazilian blood donors reveals different regional frequencies of the HY allele |
| title_sort | dombrock genotyping in brazilian blood donors reveals different regional frequencies of the hy allele |
| topic | Blood donors Polymerase chain reaction Genotyping techniques Blood group antigens Phenotype H-Y antigen/blood Polymorphism, restriction fragment length Alleles Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842013000600400&lng=en&tlng=en |
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