Adaptive lifestyle of bacteria determines phage-bacteria interaction
Bacteriophages and their interactions with microbes are not well understood. As a first step toward achieving a better understanding, we isolated and sequenced the Curvibacter phage PCA1 for the purpose of eliminating Curvibacter sp. AEP1.3, the main colonizer of Hydra vulgaris AEP. Our experiments...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1056388/full |
| _version_ | 1811185877202763776 |
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| author | Laura Ulrich Christoph Giez Leon X. Steiner Ute Hentschel Tim Lachnit |
| author_facet | Laura Ulrich Christoph Giez Leon X. Steiner Ute Hentschel Tim Lachnit |
| author_sort | Laura Ulrich |
| collection | DOAJ |
| description | Bacteriophages and their interactions with microbes are not well understood. As a first step toward achieving a better understanding, we isolated and sequenced the Curvibacter phage PCA1 for the purpose of eliminating Curvibacter sp. AEP1.3, the main colonizer of Hydra vulgaris AEP. Our experiments showed that PCA1 phage caused a strong, virulent infection only in sessile Curvibacter sp. AEP1.3 but was unable to infect planktonic and host-associated bacterial cells of the same strain. In an effort to investigate this phenomenon, we compared sessile, planktonic, and host-associated bacteria via RNA sequencing and found that all three states differed significantly in their expression patterns. This finding led us to propose that the adaptive lifestyle of Curvibacter sp. AEP1.3 results in varying degrees of susceptibility to bacteriophage infection. This concept could be relevant for phage research and phage therapy in particular. Finally, we were able to induce phage infection in planktonic cells and pinpoint the infection process to a membrane protein. We further identified potential phage-binding protein candidates based on expression pattern analysis. |
| first_indexed | 2024-04-11T13:36:41Z |
| format | Article |
| id | doaj.art-a496086ffa764765a33f71d5bb86d035 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T13:36:41Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a496086ffa764765a33f71d5bb86d0352022-12-22T04:21:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10563881056388Adaptive lifestyle of bacteria determines phage-bacteria interactionLaura Ulrich0Christoph Giez1Leon X. Steiner2Ute Hentschel3Tim Lachnit4Zoological Institute, Christian-Albrechts-Universität, Kiel, GermanyZoological Institute, Christian-Albrechts-Universität, Kiel, GermanyRD3 Marine Ecology, RU Marine Symbioses, GEOMAR Helmholtz Centre for Ocean Research, Kiel, GermanyRD3 Marine Ecology, RU Marine Symbioses, GEOMAR Helmholtz Centre for Ocean Research, Kiel, GermanyZoological Institute, Christian-Albrechts-Universität, Kiel, GermanyBacteriophages and their interactions with microbes are not well understood. As a first step toward achieving a better understanding, we isolated and sequenced the Curvibacter phage PCA1 for the purpose of eliminating Curvibacter sp. AEP1.3, the main colonizer of Hydra vulgaris AEP. Our experiments showed that PCA1 phage caused a strong, virulent infection only in sessile Curvibacter sp. AEP1.3 but was unable to infect planktonic and host-associated bacterial cells of the same strain. In an effort to investigate this phenomenon, we compared sessile, planktonic, and host-associated bacteria via RNA sequencing and found that all three states differed significantly in their expression patterns. This finding led us to propose that the adaptive lifestyle of Curvibacter sp. AEP1.3 results in varying degrees of susceptibility to bacteriophage infection. This concept could be relevant for phage research and phage therapy in particular. Finally, we were able to induce phage infection in planktonic cells and pinpoint the infection process to a membrane protein. We further identified potential phage-binding protein candidates based on expression pattern analysis.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1056388/fullbacteriophagephage infectionCurvibacterbiphasic life cyclemicrobiotabacterial adaptation |
| spellingShingle | Laura Ulrich Christoph Giez Leon X. Steiner Ute Hentschel Tim Lachnit Adaptive lifestyle of bacteria determines phage-bacteria interaction Frontiers in Microbiology bacteriophage phage infection Curvibacter biphasic life cycle microbiota bacterial adaptation |
| title | Adaptive lifestyle of bacteria determines phage-bacteria interaction |
| title_full | Adaptive lifestyle of bacteria determines phage-bacteria interaction |
| title_fullStr | Adaptive lifestyle of bacteria determines phage-bacteria interaction |
| title_full_unstemmed | Adaptive lifestyle of bacteria determines phage-bacteria interaction |
| title_short | Adaptive lifestyle of bacteria determines phage-bacteria interaction |
| title_sort | adaptive lifestyle of bacteria determines phage bacteria interaction |
| topic | bacteriophage phage infection Curvibacter biphasic life cycle microbiota bacterial adaptation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1056388/full |
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