Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria
To investigate the ultrastructural changes, X-ray microanalysis and leakages of protein and lipids from bacteria treated with the ethanolic extract of Acacia mearnsii. The ultrastructural changes were investigated using scanning electron microscope (SEM). The X-ray microanalyses were determined u...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/ultrastructure-and-x-ray-microanalysis-of-the-antibacterial-effects-of-stem-bark-ethanol-extract-of-acacia-mearnsii-de-wild-against-some-selected-bacteria/ |
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| author | Olufunmiso O. Olajuyigbe Aderonke A. Olajuyigbe Anthony J. Afolayan |
| author_facet | Olufunmiso O. Olajuyigbe Aderonke A. Olajuyigbe Anthony J. Afolayan |
| author_sort | Olufunmiso O. Olajuyigbe |
| collection | DOAJ |
| description | To investigate the ultrastructural changes, X-ray microanalysis and leakages of protein and lipids
from bacteria treated with the ethanolic extract of Acacia mearnsii. The ultrastructural changes were
investigated using scanning electron microscope (SEM). The X-ray microanalyses were determined
using electron dispersive spectroscopy (EDS) while the protein and lipid leakages were determined
spectrophotometrically. The scanning electron microscopy indicated varied ultrastructural changes in
the morphology of bacterial cells treated with the ethanol extract. The X-ray microanalysis showed
significant differences between the elemental contents of extract-treated and broth-cultured and
untreated bacteria strains. Lipids and proteins were leaked to a greater extent from the extract-treated
bacterial strains in comparison with the broth-cultured and untreated ones used as controls. The
possible antibacterial effects of the ethanol extract of A. mearnsii could be inhibition of a significant
step in peptidoglycan assembly, inhibition of metabolic processes as well as the cell wall and cell
membrane disruptions resulting in the efflux of lipid and protein in all the bacteria tested. This is a
report identifying elemental components and associating their disintegrations in bacterial isolates
with the antibacterial activities of medicinal plants. While ethanol extract of A. mearnsii caused
ultrastructural changes, breakdown of elemental components into different elements and leaked
lipid and protein from treated bacteria, the physiological damages to the cell wall further justify the
bactericidal ability of the extract. |
| first_indexed | 2024-12-14T05:03:33Z |
| format | Article |
| id | doaj.art-a4991080080b428ebf3828d82396f417 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-14T05:03:33Z |
| publishDate | 2018-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-a4991080080b428ebf3828d82396f4172022-12-21T23:16:11ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011242217222810.22207/JPAM.12.4.61Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected BacteriaOlufunmiso O. Olajuyigbe0Aderonke A. Olajuyigbe1Anthony J. Afolayan2Medicinal Plant and Economic Development (MPED) Research Centre, Department of Botany, University of Fort Hare, Alice 5700, South Africa.Medicinal Plant and Economic Development (MPED) Research Centre, Department of Botany, University of Fort Hare, Alice 5700, South Africa.Medicinal Plant and Economic Development (MPED) Research Centre, Department of Botany, University of Fort Hare, Alice 5700, South Africa.To investigate the ultrastructural changes, X-ray microanalysis and leakages of protein and lipids from bacteria treated with the ethanolic extract of Acacia mearnsii. The ultrastructural changes were investigated using scanning electron microscope (SEM). The X-ray microanalyses were determined using electron dispersive spectroscopy (EDS) while the protein and lipid leakages were determined spectrophotometrically. The scanning electron microscopy indicated varied ultrastructural changes in the morphology of bacterial cells treated with the ethanol extract. The X-ray microanalysis showed significant differences between the elemental contents of extract-treated and broth-cultured and untreated bacteria strains. Lipids and proteins were leaked to a greater extent from the extract-treated bacterial strains in comparison with the broth-cultured and untreated ones used as controls. The possible antibacterial effects of the ethanol extract of A. mearnsii could be inhibition of a significant step in peptidoglycan assembly, inhibition of metabolic processes as well as the cell wall and cell membrane disruptions resulting in the efflux of lipid and protein in all the bacteria tested. This is a report identifying elemental components and associating their disintegrations in bacterial isolates with the antibacterial activities of medicinal plants. While ethanol extract of A. mearnsii caused ultrastructural changes, breakdown of elemental components into different elements and leaked lipid and protein from treated bacteria, the physiological damages to the cell wall further justify the bactericidal ability of the extract.https://microbiologyjournal.org/ultrastructure-and-x-ray-microanalysis-of-the-antibacterial-effects-of-stem-bark-ethanol-extract-of-acacia-mearnsii-de-wild-against-some-selected-bacteria/ultrastructural changesmicroanalysiselemental contentsphysiological damages |
| spellingShingle | Olufunmiso O. Olajuyigbe Aderonke A. Olajuyigbe Anthony J. Afolayan Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria Journal of Pure and Applied Microbiology ultrastructural changes microanalysis elemental contents physiological damages |
| title | Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria |
| title_full | Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria |
| title_fullStr | Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria |
| title_full_unstemmed | Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria |
| title_short | Ultrastructure and X-ray Microanalysis of the Antibacterial Effects of Stem Bark Ethanol Extract of Acacia mearnsii De Wild Against Some Selected Bacteria |
| title_sort | ultrastructure and x ray microanalysis of the antibacterial effects of stem bark ethanol extract of acacia mearnsii de wild against some selected bacteria |
| topic | ultrastructural changes microanalysis elemental contents physiological damages |
| url | https://microbiologyjournal.org/ultrastructure-and-x-ray-microanalysis-of-the-antibacterial-effects-of-stem-bark-ethanol-extract-of-acacia-mearnsii-de-wild-against-some-selected-bacteria/ |
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