Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline
Vibrio species are an important and widespread component of marine microbial communities. Some Vibrio strains are potentially pathogenic to marine vertebrates and invertebrates. The aim of this study was to identify vibrios, in particular Vibrio splendidus and related species, isolated from clams (...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2011-09-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2011/47_3/371.pdf |
| _version_ | 1811196927185780736 |
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| author | Marina Torresi Vicdalia A. Acciari Annamaria Piano Patrizia Serratore Vincenza Prencipe Giacomo Migliorati |
| author_facet | Marina Torresi Vicdalia A. Acciari Annamaria Piano Patrizia Serratore Vincenza Prencipe Giacomo Migliorati |
| author_sort | Marina Torresi |
| collection | DOAJ |
| description | Vibrio species are an important and widespread component of marine microbial communities. Some Vibrio strains are potentially pathogenic to marine vertebrates and invertebrates. The aim of this study was to identify vibrios, in particular Vibrio splendidus and related species, isolated from clams (Chamelea gallina) collected along the coasts of the Abruzzi region from May to October 2007. The isolates obtained were phenotyped and classified as belonging to the genus Vibrio. The strains underwent biochemical testing in accordance with Alsina’s scheme for V. splendidus identification. Molecular analysis of the 16S-23S intergenic space region and recA gene was used to identify V. splendidus and related species. All the samples examined were found to contain halophylic Vibrio species, with V. alginolyticus, V. splendidus-related species and V. mediterranei most commonly found. A polymerase chain reaction of the 16S-23S intergenic space region and sequencing of the recA gene from isolates confirmed that phenotyping of Vibrio species is not sufficient to distinguish between different species. Differentiation of the highly related species among V. splendidus-related clusters remains an important issue. In this regard, our data suggests sequencing the recA genes was far more discriminatory than sequencing 16S rDNA for this purpose. |
| first_indexed | 2024-04-12T01:06:53Z |
| format | Article |
| id | doaj.art-a49a449619584079a33de70f782c5d0f |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-12T01:06:53Z |
| publishDate | 2011-09-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-a49a449619584079a33de70f782c5d0f2022-12-22T03:54:14ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272011-09-01473371378Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastlineMarina TorresiVicdalia A. AcciariAnnamaria PianoPatrizia SerratoreVincenza PrencipeGiacomo MiglioratiVibrio species are an important and widespread component of marine microbial communities. Some Vibrio strains are potentially pathogenic to marine vertebrates and invertebrates. The aim of this study was to identify vibrios, in particular Vibrio splendidus and related species, isolated from clams (Chamelea gallina) collected along the coasts of the Abruzzi region from May to October 2007. The isolates obtained were phenotyped and classified as belonging to the genus Vibrio. The strains underwent biochemical testing in accordance with Alsina’s scheme for V. splendidus identification. Molecular analysis of the 16S-23S intergenic space region and recA gene was used to identify V. splendidus and related species. All the samples examined were found to contain halophylic Vibrio species, with V. alginolyticus, V. splendidus-related species and V. mediterranei most commonly found. A polymerase chain reaction of the 16S-23S intergenic space region and sequencing of the recA gene from isolates confirmed that phenotyping of Vibrio species is not sufficient to distinguish between different species. Differentiation of the highly related species among V. splendidus-related clusters remains an important issue. In this regard, our data suggests sequencing the recA genes was far more discriminatory than sequencing 16S rDNA for this purpose.http://www.izs.it/vet_italiana/2011/47_3/371.pdfAbruzziAdriatic SeaChamelea gallinaItalyRecAVibrio splendidusVibrio spp. |
| spellingShingle | Marina Torresi Vicdalia A. Acciari Annamaria Piano Patrizia Serratore Vincenza Prencipe Giacomo Migliorati Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline Veterinaria Italiana Abruzzi Adriatic Sea Chamelea gallina Italy RecA Vibrio splendidus Vibrio spp. |
| title | Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline |
| title_full | Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline |
| title_fullStr | Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline |
| title_full_unstemmed | Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline |
| title_short | Detection of Vibrio splendidus and related species in Chamelea gallina sampled in the Adriatic along the Abruzzi coastline |
| title_sort | detection of vibrio splendidus and related species in chamelea gallina sampled in the adriatic along the abruzzi coastline |
| topic | Abruzzi Adriatic Sea Chamelea gallina Italy RecA Vibrio splendidus Vibrio spp. |
| url | http://www.izs.it/vet_italiana/2011/47_3/371.pdf |
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