Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications
Emerging and re-emerging swine coronaviruses (CoVs), including porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome-CoV (SADS-CoV), cause severe diarrhea in neonatal piglets, and CoV infection is associated with significant economic losses for t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/10/2003 |
| _version_ | 1797572043802148864 |
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| author | Hui Jiang Ting Wang Lingbao Kong Bin Li Qi Peng |
| author_facet | Hui Jiang Ting Wang Lingbao Kong Bin Li Qi Peng |
| author_sort | Hui Jiang |
| collection | DOAJ |
| description | Emerging and re-emerging swine coronaviruses (CoVs), including porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome-CoV (SADS-CoV), cause severe diarrhea in neonatal piglets, and CoV infection is associated with significant economic losses for the swine industry worldwide. Reverse genetics systems realize the manipulation of RNA virus genome and facilitate the development of new vaccines. Thus far, five reverse genetics approaches have been successfully applied to engineer the swine CoV genome: targeted RNA recombination, in vitro ligation, bacterial artificial chromosome-based ligation, vaccinia virus -based recombination, and yeast-based method. This review summarizes the advantages and limitations of these approaches; it also discusses the latest research progress in terms of their use for virus-related pathogenesis elucidation, vaccine candidate development, antiviral drug screening, and virus replication mechanism determination. |
| first_indexed | 2024-03-10T20:50:06Z |
| format | Article |
| id | doaj.art-a49c7d38e352449a815206cbd1b13cdc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:50:06Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a49c7d38e352449a815206cbd1b13cdc2023-11-19T18:26:46ZengMDPI AGViruses1999-49152023-09-011510200310.3390/v15102003Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and ApplicationsHui Jiang0Ting Wang1Lingbao Kong2Bin Li3Qi Peng4Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, ChinaNanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, ChinaNanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, ChinaNanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, ChinaEmerging and re-emerging swine coronaviruses (CoVs), including porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome-CoV (SADS-CoV), cause severe diarrhea in neonatal piglets, and CoV infection is associated with significant economic losses for the swine industry worldwide. Reverse genetics systems realize the manipulation of RNA virus genome and facilitate the development of new vaccines. Thus far, five reverse genetics approaches have been successfully applied to engineer the swine CoV genome: targeted RNA recombination, in vitro ligation, bacterial artificial chromosome-based ligation, vaccinia virus -based recombination, and yeast-based method. This review summarizes the advantages and limitations of these approaches; it also discusses the latest research progress in terms of their use for virus-related pathogenesis elucidation, vaccine candidate development, antiviral drug screening, and virus replication mechanism determination.https://www.mdpi.com/1999-4915/15/10/2003emerging and re-emerging swine coronavirusesreverse genetics systempathogenesisvaccine developmentantiviral drugscell and tissue tropism |
| spellingShingle | Hui Jiang Ting Wang Lingbao Kong Bin Li Qi Peng Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications Viruses emerging and re-emerging swine coronaviruses reverse genetics system pathogenesis vaccine development antiviral drugs cell and tissue tropism |
| title | Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications |
| title_full | Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications |
| title_fullStr | Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications |
| title_full_unstemmed | Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications |
| title_short | Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications |
| title_sort | reverse genetics systems for emerging and re emerging swine coronaviruses and applications |
| topic | emerging and re-emerging swine coronaviruses reverse genetics system pathogenesis vaccine development antiviral drugs cell and tissue tropism |
| url | https://www.mdpi.com/1999-4915/15/10/2003 |
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