Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period
Pregnancy diagnosis during early gestation is important for cattle reproduction. The expression of interferon-stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) was studied in embryo-transferred (ET) Japanese Black cattle. ISGs in PBLs—<i>ISG15</i>, <i>MX1</i>, <...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/7/408 |
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| author | Hitomi Yoshino Keiichiro Kizaki Toh-ichi Hirata Kosuke Iga Hideo Matsuda Tadayuki Yamanouchi Yutaka Hashiyada Kei Imai Toshina Ishiguro-Oonuma Tomomi Kanazawa Toru Takahashi Kazuyoshi Hashizume |
| author_facet | Hitomi Yoshino Keiichiro Kizaki Toh-ichi Hirata Kosuke Iga Hideo Matsuda Tadayuki Yamanouchi Yutaka Hashiyada Kei Imai Toshina Ishiguro-Oonuma Tomomi Kanazawa Toru Takahashi Kazuyoshi Hashizume |
| author_sort | Hitomi Yoshino |
| collection | DOAJ |
| description | Pregnancy diagnosis during early gestation is important for cattle reproduction. The expression of interferon-stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) was studied in embryo-transferred (ET) Japanese Black cattle. ISGs in PBLs—<i>ISG15</i>, <i>MX1</i>, <i>MX2</i>, and <i>OAS1</i>—were detected in multiple ovulation ET cattle using a real-time quantitative polymerase chain reaction, and receiver operating characteristic (ROC) curve analysis was performed. Gestational status was predicted using the average ISG levels during the normal estrous cycle (AVE) and the Youden index from the ROC curve analysis as cutoff values. The <i>ISG15</i>, <i>MX1</i>, and <i>MX2</i> levels were significantly higher in pregnant cattle (<i>n</i> = 10) than in non-pregnant cattle (<i>n</i> = 23) on gestation day 21, whereas the levels of all ISGs were similar between non-pregnant and non-pregnant cattle with late embryonic death (<i>n</i> = 7). <i>ISG15</i>, <i>MX1</i>, and <i>MX2</i> appropriately predicted the gestational status of ET cows. The statistical evaluation of the diagnostic accuracy in ET cows on day 21 of gestation presented higher values of sensitivity, specificity, accuracy, and positive predictive values of <i>ISG15, MX1</i>, and <i>MX2</i> using the Youden index than using the AVE. Therefore, <i>ISG15</i>, <i>MX1</i>, and <i>MX2</i> are excellent biomarkers of gestational status during the peri-implantation period in ET cattle. |
| first_indexed | 2024-03-11T00:34:26Z |
| format | Article |
| id | doaj.art-a49cea77c73b40809e37f30f3fa0b189 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T00:34:26Z |
| publishDate | 2023-06-01 |
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| series | Veterinary Sciences |
| spelling | doaj.art-a49cea77c73b40809e37f30f3fa0b1892023-11-18T21:42:10ZengMDPI AGVeterinary Sciences2306-73812023-06-0110740810.3390/vetsci10070408Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation PeriodHitomi Yoshino0Keiichiro Kizaki1Toh-ichi Hirata2Kosuke Iga3Hideo Matsuda4Tadayuki Yamanouchi5Yutaka Hashiyada6Kei Imai7Toshina Ishiguro-Oonuma8Tomomi Kanazawa9Toru Takahashi10Kazuyoshi Hashizume11Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanCooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanField Science Center, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanTohoku Agricultural Research Center, National Agriculture and Food Research Organization, 4 Akahira, Shimo-kuriyagawa, Morioka 020-0198, Iwate, JapanNational Livestock Breeding Center, Nishigo 961-8061, Fukushima, JapanNational Livestock Breeding Center, Nishigo 961-8061, Fukushima, JapanNational Livestock Breeding Center, Nishigo 961-8061, Fukushima, JapanDepartment of Sustainable Agriculture, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu 069-8501, Hokkaido, JapanCooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanCooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanCooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanCooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Iwate, JapanPregnancy diagnosis during early gestation is important for cattle reproduction. The expression of interferon-stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) was studied in embryo-transferred (ET) Japanese Black cattle. ISGs in PBLs—<i>ISG15</i>, <i>MX1</i>, <i>MX2</i>, and <i>OAS1</i>—were detected in multiple ovulation ET cattle using a real-time quantitative polymerase chain reaction, and receiver operating characteristic (ROC) curve analysis was performed. Gestational status was predicted using the average ISG levels during the normal estrous cycle (AVE) and the Youden index from the ROC curve analysis as cutoff values. The <i>ISG15</i>, <i>MX1</i>, and <i>MX2</i> levels were significantly higher in pregnant cattle (<i>n</i> = 10) than in non-pregnant cattle (<i>n</i> = 23) on gestation day 21, whereas the levels of all ISGs were similar between non-pregnant and non-pregnant cattle with late embryonic death (<i>n</i> = 7). <i>ISG15</i>, <i>MX1</i>, and <i>MX2</i> appropriately predicted the gestational status of ET cows. The statistical evaluation of the diagnostic accuracy in ET cows on day 21 of gestation presented higher values of sensitivity, specificity, accuracy, and positive predictive values of <i>ISG15, MX1</i>, and <i>MX2</i> using the Youden index than using the AVE. Therefore, <i>ISG15</i>, <i>MX1</i>, and <i>MX2</i> are excellent biomarkers of gestational status during the peri-implantation period in ET cattle.https://www.mdpi.com/2306-7381/10/7/408pregnancy diagnosisgestational statusbiomarker |
| spellingShingle | Hitomi Yoshino Keiichiro Kizaki Toh-ichi Hirata Kosuke Iga Hideo Matsuda Tadayuki Yamanouchi Yutaka Hashiyada Kei Imai Toshina Ishiguro-Oonuma Tomomi Kanazawa Toru Takahashi Kazuyoshi Hashizume Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period Veterinary Sciences pregnancy diagnosis gestational status biomarker |
| title | Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period |
| title_full | Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period |
| title_fullStr | Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period |
| title_full_unstemmed | Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period |
| title_short | Interferon-Stimulated Gene Expression in Peripheral Blood Leucocytes as a Convenient Prediction Marker for Embryo Status in Embryo-Transferred Japanese Black Cows during the Peri-Implantation Period |
| title_sort | interferon stimulated gene expression in peripheral blood leucocytes as a convenient prediction marker for embryo status in embryo transferred japanese black cows during the peri implantation period |
| topic | pregnancy diagnosis gestational status biomarker |
| url | https://www.mdpi.com/2306-7381/10/7/408 |
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