Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway
Fish gills are the major osmoregulatory tissue that contact the external water environment and have developed an effective osmoregulatory mechanism to maintain cellular function. Marine medaka (<i>Oryzias melastigma</i>) has the ability to live in both seawater and fresh water environmen...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12417 |
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| author | Rong Li Jiaqi Liu Chi Tim Leung Xiao Lin Ting Fung Chan William Ka Fai Tse Keng Po Lai |
| author_facet | Rong Li Jiaqi Liu Chi Tim Leung Xiao Lin Ting Fung Chan William Ka Fai Tse Keng Po Lai |
| author_sort | Rong Li |
| collection | DOAJ |
| description | Fish gills are the major osmoregulatory tissue that contact the external water environment and have developed an effective osmoregulatory mechanism to maintain cellular function. Marine medaka (<i>Oryzias melastigma</i>) has the ability to live in both seawater and fresh water environments. The present study performed a seawater (SW) to 50% seawater (SFW) transfer, and the gill samples were used for comparative transcriptomic analysis to study the alteration of hypo-osmotic stress on immune responsive genes in this model organism. The result identified 518 differentiated expressed genes (DEGs) after the SW to SFW transfer. Various pathways such as p53 signaling, forkhead box O signaling, and the cell cycle were enriched. Moreover, the immune system was highlighted as one of the top altered biological processes in the enrichment analysis. Various cytokines, chemokines, and inflammatory genes that participate in the IL-17 signaling pathway were suppressed after the SW to SFW transfer. On the other hand, some immunoglobulin-related genes were up-regulated. The results were further validated by real-time qPCR. Taken together, our study provides additional gill transcriptome information in marine medaka; it also supports the notion that osmotic stress could influence the immune responses in fish gills. |
| first_indexed | 2024-03-09T20:06:02Z |
| format | Article |
| id | doaj.art-a49d3feeb848476081f3a7f71336a3a1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:06:02Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a49d3feeb848476081f3a7f71336a3a12023-11-24T00:31:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201241710.3390/ijms232012417Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling PathwayRong Li0Jiaqi Liu1Chi Tim Leung2Xiao Lin3Ting Fung Chan4William Ka Fai Tse5Keng Po Lai6Key Laboratory of Environmental Pollution and Integrative Omics, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Huan Cheng North 2nd Road 109, Guilin 541004, ChinaKey Laboratory of Environmental Pollution and Integrative Omics, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Huan Cheng North 2nd Road 109, Guilin 541004, ChinaDepartment of Chemistry, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USAState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, ChinaLaboratory of Developmental Disorders and Toxicology, Center for Promotion of International Education and Research, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, JapanKey Laboratory of Environmental Pollution and Integrative Omics, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, Huan Cheng North 2nd Road 109, Guilin 541004, ChinaFish gills are the major osmoregulatory tissue that contact the external water environment and have developed an effective osmoregulatory mechanism to maintain cellular function. Marine medaka (<i>Oryzias melastigma</i>) has the ability to live in both seawater and fresh water environments. The present study performed a seawater (SW) to 50% seawater (SFW) transfer, and the gill samples were used for comparative transcriptomic analysis to study the alteration of hypo-osmotic stress on immune responsive genes in this model organism. The result identified 518 differentiated expressed genes (DEGs) after the SW to SFW transfer. Various pathways such as p53 signaling, forkhead box O signaling, and the cell cycle were enriched. Moreover, the immune system was highlighted as one of the top altered biological processes in the enrichment analysis. Various cytokines, chemokines, and inflammatory genes that participate in the IL-17 signaling pathway were suppressed after the SW to SFW transfer. On the other hand, some immunoglobulin-related genes were up-regulated. The results were further validated by real-time qPCR. Taken together, our study provides additional gill transcriptome information in marine medaka; it also supports the notion that osmotic stress could influence the immune responses in fish gills.https://www.mdpi.com/1422-0067/23/20/12417cell cyclep53 pathwayFoxO signalingimmune systemgill mucosal immunity |
| spellingShingle | Rong Li Jiaqi Liu Chi Tim Leung Xiao Lin Ting Fung Chan William Ka Fai Tse Keng Po Lai Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway International Journal of Molecular Sciences cell cycle p53 pathway FoxO signaling immune system gill mucosal immunity |
| title | Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway |
| title_full | Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway |
| title_fullStr | Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway |
| title_full_unstemmed | Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway |
| title_short | Transcriptomic Analysis in Marine Medaka Gill Reveals That the Hypo-Osmotic Stress Could Alter the Immune Response via the IL17 Signaling Pathway |
| title_sort | transcriptomic analysis in marine medaka gill reveals that the hypo osmotic stress could alter the immune response via the il17 signaling pathway |
| topic | cell cycle p53 pathway FoxO signaling immune system gill mucosal immunity |
| url | https://www.mdpi.com/1422-0067/23/20/12417 |
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