Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection. With the participation of Bst 2.0 DNA Polymerase, the method enabled RCA and LAMP amplif...

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Bibliographic Details
Main Authors: Zheng Cao, Xianfeng Jiang, Guizhou Xiao, Mingcheng Xu, Hui Liu, Sheng Cai
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Separations
Subjects:
Online Access:https://www.mdpi.com/2297-8739/8/10/166