The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes
The rimb1 (redox imbalanced 1) mutation was mapped to the RCD1 locus (radical-induced cell death 1; At1g32230) demonstrating that a major factor involved in redox-regulation genes for chloroplast antioxidant enzymes and protection against photooxidative stress, RIMB1, is identical to the regulator o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00475/full |
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| author | Heiko eHiltscher Radoslaw eRudnik Jehad eShaikhali Isabelle eHeiber Marina eMellenthin Iuri eMeirelles Duarte Guenter eSchuster Uwe eKahmann Margarete eBaier |
| author_facet | Heiko eHiltscher Radoslaw eRudnik Jehad eShaikhali Isabelle eHeiber Marina eMellenthin Iuri eMeirelles Duarte Guenter eSchuster Uwe eKahmann Margarete eBaier |
| author_sort | Heiko eHiltscher |
| collection | DOAJ |
| description | The rimb1 (redox imbalanced 1) mutation was mapped to the RCD1 locus (radical-induced cell death 1; At1g32230) demonstrating that a major factor involved in redox-regulation genes for chloroplast antioxidant enzymes and protection against photooxidative stress, RIMB1, is identical to the regulator of disease response reactions and cell death, RCD1.Discovering this link let to our investigation of its regulatory mechanism. We show in yeast that RCD1 can physically interact with the transcription factor Rap2.4a which provides redox-sensitivity to nuclear expression of genes for chloroplast antioxidant enzymes. In the rimb1 (rcd1-6) mutant a single nucleotide exchange results in a truncated RCD1 protein lacking the transcription factor binding site. Protein-protein interaction between full-length RCD1 and Rap2.4a is supported by H2O2, but not sensitive to the antioxidants dithiotreitol and ascorbate. In combination with transcript abundance analysis in Arabidopsis, it is concluded that RCD1 stabilizes the Rap2.4-dependent redox-regulation of the genes encoding chloroplast antioxidant enzymes in a widely redox-independent manner.Over the years, rcd1-mutant alleles have been described to develop symptoms like chlorosis, lesions along the leaf rims and in the mesophyll and (secondary) induction of extra- and intra-plastidic antioxidant defense mechanisms. All these rcd1 mutant characteristics were observed in rcd1-6 to succeed low activation of the chloroplast antioxidant system and glutathione biosynthesis. We conclude that RCD1 protects plant cells from running into ROS (reactive oxygen species)-triggered programs, such as cell death and activation of pathogen-responsive genes (PR genes) and extra-plastidic antioxidant enzymes, by supporting the induction of the chloroplast antioxidant system. |
| first_indexed | 2024-12-23T04:36:53Z |
| format | Article |
| id | doaj.art-a49fa7618b7f457fb04901c92279798f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T04:36:53Z |
| publishDate | 2014-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a49fa7618b7f457fb04901c92279798f2022-12-21T17:59:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-09-01510.3389/fpls.2014.00475102006The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymesHeiko eHiltscher0Radoslaw eRudnik1Jehad eShaikhali2Isabelle eHeiber3Marina eMellenthin4Iuri eMeirelles Duarte5Guenter eSchuster6Uwe eKahmann7Margarete eBaier8Heinrich-Heine-UniversityFU BerlinBielefeld UniversityBielefeld UniversityHeinrich-Heine-UniversityHeinrich-Heine-UniversityHeinrich-Heine-UniversityBielefeld UniversityFU BerlinThe rimb1 (redox imbalanced 1) mutation was mapped to the RCD1 locus (radical-induced cell death 1; At1g32230) demonstrating that a major factor involved in redox-regulation genes for chloroplast antioxidant enzymes and protection against photooxidative stress, RIMB1, is identical to the regulator of disease response reactions and cell death, RCD1.Discovering this link let to our investigation of its regulatory mechanism. We show in yeast that RCD1 can physically interact with the transcription factor Rap2.4a which provides redox-sensitivity to nuclear expression of genes for chloroplast antioxidant enzymes. In the rimb1 (rcd1-6) mutant a single nucleotide exchange results in a truncated RCD1 protein lacking the transcription factor binding site. Protein-protein interaction between full-length RCD1 and Rap2.4a is supported by H2O2, but not sensitive to the antioxidants dithiotreitol and ascorbate. In combination with transcript abundance analysis in Arabidopsis, it is concluded that RCD1 stabilizes the Rap2.4-dependent redox-regulation of the genes encoding chloroplast antioxidant enzymes in a widely redox-independent manner.Over the years, rcd1-mutant alleles have been described to develop symptoms like chlorosis, lesions along the leaf rims and in the mesophyll and (secondary) induction of extra- and intra-plastidic antioxidant defense mechanisms. All these rcd1 mutant characteristics were observed in rcd1-6 to succeed low activation of the chloroplast antioxidant system and glutathione biosynthesis. We conclude that RCD1 protects plant cells from running into ROS (reactive oxygen species)-triggered programs, such as cell death and activation of pathogen-responsive genes (PR genes) and extra-plastidic antioxidant enzymes, by supporting the induction of the chloroplast antioxidant system.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00475/fullArabidopsischloroplastROSantioxidant systemRCD1 |
| spellingShingle | Heiko eHiltscher Radoslaw eRudnik Jehad eShaikhali Isabelle eHeiber Marina eMellenthin Iuri eMeirelles Duarte Guenter eSchuster Uwe eKahmann Margarete eBaier The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes Frontiers in Plant Science Arabidopsis chloroplast ROS antioxidant system RCD1 |
| title | The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes |
| title_full | The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes |
| title_fullStr | The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes |
| title_full_unstemmed | The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes |
| title_short | The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes |
| title_sort | radical induced cell death protein 1 rcd1 supports transcriptional activation of genes for chloroplast antioxidant enzymes |
| topic | Arabidopsis chloroplast ROS antioxidant system RCD1 |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00475/full |
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