A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration
Microglial cells are the key regulators of inflammation during retinal degeneration (RD) and are conventionally classified as M1 or M2. However, whether the M1/M2 classification exactly reflects the functional classification of microglial cells in the retina remains debatable. We examined the spatio...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14236 |
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| author | Yan Zhang Yong Soo Park In-Beom Kim |
| author_facet | Yan Zhang Yong Soo Park In-Beom Kim |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | Microglial cells are the key regulators of inflammation during retinal degeneration (RD) and are conventionally classified as M1 or M2. However, whether the M1/M2 classification exactly reflects the functional classification of microglial cells in the retina remains debatable. We examined the spatiotemporal changes of microglial cells in the blue-LED and NaIO<sub>3</sub>-induced RD mice models using M1/M2 markers and functional genes. TUNEL assay was performed to detect photoreceptor cell death, and microglial cells were labeled with anti-IBA1, P2RY12, CD86, and CD206 antibodies. FACS was used to isolate microglial cells with anti-CD206 and CD86 antibodies, and qRT-PCR was performed to evaluate <i>Il-10</i>, <i>Il-6</i>, <i>Trem-2</i>, <i>Apoe</i>, and <i>Lyz2</i> expression. TUNEL-positive cells were detected in the outer nuclear layer (ONL) from 24 h to 72 h post-RD induction. At 24 h, P2RY12 was decreased and CD86 was increased, and CD86/CD206 double-labeled cells occupied the dominant population at 72 h. And CD86/CD206 double-labeled cells showed a significant increase in <i>Apoe</i>, <i>Trem2</i>, and <i>Lyz2</i> levels but not in those of <i>Il-6</i> and <i>Il-10</i>. Our results demonstrate that microglial cells in active RD cannot be classified as M1 or M2, and the majority of microglia express both CD86 and CD206, which are involved in phagocytosis rather than inflammation. |
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| issn | 1661-6596 1422-0067 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a4a0e2a3cb184fd1b58a811a300bc7f52023-11-19T11:09:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181423610.3390/ijms241814236A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal DegenerationYan Zhang0Yong Soo Park1In-Beom Kim2Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaMicroglial cells are the key regulators of inflammation during retinal degeneration (RD) and are conventionally classified as M1 or M2. However, whether the M1/M2 classification exactly reflects the functional classification of microglial cells in the retina remains debatable. We examined the spatiotemporal changes of microglial cells in the blue-LED and NaIO<sub>3</sub>-induced RD mice models using M1/M2 markers and functional genes. TUNEL assay was performed to detect photoreceptor cell death, and microglial cells were labeled with anti-IBA1, P2RY12, CD86, and CD206 antibodies. FACS was used to isolate microglial cells with anti-CD206 and CD86 antibodies, and qRT-PCR was performed to evaluate <i>Il-10</i>, <i>Il-6</i>, <i>Trem-2</i>, <i>Apoe</i>, and <i>Lyz2</i> expression. TUNEL-positive cells were detected in the outer nuclear layer (ONL) from 24 h to 72 h post-RD induction. At 24 h, P2RY12 was decreased and CD86 was increased, and CD86/CD206 double-labeled cells occupied the dominant population at 72 h. And CD86/CD206 double-labeled cells showed a significant increase in <i>Apoe</i>, <i>Trem2</i>, and <i>Lyz2</i> levels but not in those of <i>Il-6</i> and <i>Il-10</i>. Our results demonstrate that microglial cells in active RD cannot be classified as M1 or M2, and the majority of microglia express both CD86 and CD206, which are involved in phagocytosis rather than inflammation.https://www.mdpi.com/1422-0067/24/18/14236microgliaCD86CD206phagocytosisretinal degeneration |
| spellingShingle | Yan Zhang Yong Soo Park In-Beom Kim A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration International Journal of Molecular Sciences microglia CD86 CD206 phagocytosis retinal degeneration |
| title | A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration |
| title_full | A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration |
| title_fullStr | A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration |
| title_full_unstemmed | A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration |
| title_short | A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration |
| title_sort | distinct microglial cell population expressing both cd86 and cd206 constitutes a dominant type and executes phagocytosis in two mouse models of retinal degeneration |
| topic | microglia CD86 CD206 phagocytosis retinal degeneration |
| url | https://www.mdpi.com/1422-0067/24/18/14236 |
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