The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa
The pathogenic bacterium Pseudomonas aeruginosa is notorious for causing acute and chronic infections in humans. The ability to infect host by P. aeruginosa is dependent on a complex cellular signaling network, which includes a large number of chemosensory signaling pathways that rely on the methyl-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00067/full |
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| author | Shuo Sheng Shuo Sheng Lingyi Xin Lingyi Xin Joey Kuok Hoong Yam Joey Kuok Hoong Yam Joey Kuok Hoong Yam May Margarette Salido May Margarette Salido May Margarette Salido Nicole Zi Jia Khong Qiong Liu Qiong Liu Rachel Andrea Chea Hoi Yeung Li Liang Yang Liang Yang Liang Yang Zhao-Xun Liang Zhao-Xun Liang Linghui Xu Linghui Xu |
| author_facet | Shuo Sheng Shuo Sheng Lingyi Xin Lingyi Xin Joey Kuok Hoong Yam Joey Kuok Hoong Yam Joey Kuok Hoong Yam May Margarette Salido May Margarette Salido May Margarette Salido Nicole Zi Jia Khong Qiong Liu Qiong Liu Rachel Andrea Chea Hoi Yeung Li Liang Yang Liang Yang Liang Yang Zhao-Xun Liang Zhao-Xun Liang Linghui Xu Linghui Xu |
| author_sort | Shuo Sheng |
| collection | DOAJ |
| description | The pathogenic bacterium Pseudomonas aeruginosa is notorious for causing acute and chronic infections in humans. The ability to infect host by P. aeruginosa is dependent on a complex cellular signaling network, which includes a large number of chemosensory signaling pathways that rely on the methyl-accepting chemotaxis proteins (MCPs). We previously found that the second messenger c-di-GMP-binding adaptor MapZ modulates the methylation of an amino acid-detecting MCP by directly interacting with a chemotaxis methyltransferase CheR1. The current study further expands our understanding of the role of MapZ in regulating chemosensory pathways by demonstrating that MapZ suppresses the methylation of multiple MCPs in P. aeruginosa PAO1. The MCPs under the control of MapZ include five MCPs (Aer, CtpH, CptM, PctA, and PctB) for detecting oxygen/energy, inorganic phosphate, malate and amino acids, and three MCPs (PA1251, PA1608, and PA2867) for detecting unknown chemoattractant or chemorepellent. Chemotaxis assays showed that overexpression of MapZ hampered the taxis of P. aeruginosa toward chemoattractants and scratch-wounded human cells. Mouse infection experiments demonstrated that a dysfunction in MapZ regulation had a profound negative impact on the dissemination of P. aeruginosa and resulted in attenuated bacterial virulence. Together, the results imply that by controlling the methylation of various MCPs via the adaptor protein MapZ, c-di-GMP exerts a profound influence on chemotactic responses and bacterial pathogenesis. |
| first_indexed | 2024-12-11T15:32:42Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T15:32:42Z |
| publishDate | 2019-02-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a4a19e7637e5422980bb6cd4b877bbe32022-12-22T01:00:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00067427655The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosaShuo Sheng0Shuo Sheng1Lingyi Xin2Lingyi Xin3Joey Kuok Hoong Yam4Joey Kuok Hoong Yam5Joey Kuok Hoong Yam6May Margarette Salido7May Margarette Salido8May Margarette Salido9Nicole Zi Jia Khong10Qiong Liu11Qiong Liu12Rachel Andrea Chea13Hoi Yeung Li14Liang Yang15Liang Yang16Liang Yang17Zhao-Xun Liang18Zhao-Xun Liang19Linghui Xu20Linghui Xu21Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Innovative and Entrepreneurial Research Team of Sociomicrobiology, South China Agricultural University, Guangzhou, ChinaSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, SingaporeInterdisciplinary Graduate School, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, SingaporeInterdisciplinary Graduate School, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Innovative and Entrepreneurial Research Team of Sociomicrobiology, South China Agricultural University, Guangzhou, ChinaSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, SingaporeInterdisciplinary Graduate School, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, SingaporeGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Innovative and Entrepreneurial Research Team of Sociomicrobiology, South China Agricultural University, Guangzhou, ChinaThe pathogenic bacterium Pseudomonas aeruginosa is notorious for causing acute and chronic infections in humans. The ability to infect host by P. aeruginosa is dependent on a complex cellular signaling network, which includes a large number of chemosensory signaling pathways that rely on the methyl-accepting chemotaxis proteins (MCPs). We previously found that the second messenger c-di-GMP-binding adaptor MapZ modulates the methylation of an amino acid-detecting MCP by directly interacting with a chemotaxis methyltransferase CheR1. The current study further expands our understanding of the role of MapZ in regulating chemosensory pathways by demonstrating that MapZ suppresses the methylation of multiple MCPs in P. aeruginosa PAO1. The MCPs under the control of MapZ include five MCPs (Aer, CtpH, CptM, PctA, and PctB) for detecting oxygen/energy, inorganic phosphate, malate and amino acids, and three MCPs (PA1251, PA1608, and PA2867) for detecting unknown chemoattractant or chemorepellent. Chemotaxis assays showed that overexpression of MapZ hampered the taxis of P. aeruginosa toward chemoattractants and scratch-wounded human cells. Mouse infection experiments demonstrated that a dysfunction in MapZ regulation had a profound negative impact on the dissemination of P. aeruginosa and resulted in attenuated bacterial virulence. Together, the results imply that by controlling the methylation of various MCPs via the adaptor protein MapZ, c-di-GMP exerts a profound influence on chemotactic responses and bacterial pathogenesis.https://www.frontiersin.org/article/10.3389/fmicb.2019.00067/fullPseudomonas aeruginosachemotaxisPilZ adaptorcyclic di-GMPmethyl-accepting chemotaxis proteinmethyltransferase cheR1 |
| spellingShingle | Shuo Sheng Shuo Sheng Lingyi Xin Lingyi Xin Joey Kuok Hoong Yam Joey Kuok Hoong Yam Joey Kuok Hoong Yam May Margarette Salido May Margarette Salido May Margarette Salido Nicole Zi Jia Khong Qiong Liu Qiong Liu Rachel Andrea Chea Hoi Yeung Li Liang Yang Liang Yang Liang Yang Zhao-Xun Liang Zhao-Xun Liang Linghui Xu Linghui Xu The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa Frontiers in Microbiology Pseudomonas aeruginosa chemotaxis PilZ adaptor cyclic di-GMP methyl-accepting chemotaxis protein methyltransferase cheR1 |
| title | The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa |
| title_full | The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa |
| title_fullStr | The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa |
| title_full_unstemmed | The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa |
| title_short | The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa |
| title_sort | mapz mediated methylation of chemoreceptors contributes to pathogenicity of pseudomonas aeruginosa |
| topic | Pseudomonas aeruginosa chemotaxis PilZ adaptor cyclic di-GMP methyl-accepting chemotaxis protein methyltransferase cheR1 |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00067/full |
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