tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R
Abstract Background The drive to understand how microbial communities interact with their environments has inspired innovations across many fields. The data generated from sequence-based analyses of microbial communities typically are of high dimensionality and can involve multiple data tables consi...
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Bioinformatics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12859-021-03967-2 |
| _version_ | 1818611554101231616 |
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| author | Charlie M. Carpenter Daniel N. Frank Kayla Williamson Jaron Arbet Brandie D. Wagner Katerina Kechris Miranda E. Kroehl |
| author_facet | Charlie M. Carpenter Daniel N. Frank Kayla Williamson Jaron Arbet Brandie D. Wagner Katerina Kechris Miranda E. Kroehl |
| author_sort | Charlie M. Carpenter |
| collection | DOAJ |
| description | Abstract Background The drive to understand how microbial communities interact with their environments has inspired innovations across many fields. The data generated from sequence-based analyses of microbial communities typically are of high dimensionality and can involve multiple data tables consisting of taxonomic or functional gene/pathway counts. Merging multiple high dimensional tables with study-related metadata can be challenging. Existing microbiome pipelines available in R have created their own data structures to manage this problem. However, these data structures may be unfamiliar to analysts new to microbiome data or R and do not allow for deviations from internal workflows. Existing analysis tools also focus primarily on community-level analyses and exploratory visualizations, as opposed to analyses of individual taxa. Results We developed the R package “tidyMicro” to serve as a more complete microbiome analysis pipeline. This open source software provides all of the essential tools available in other popular packages (e.g., management of sequence count tables, standard exploratory visualizations, and diversity inference tools) supplemented with multiple options for regression modelling (e.g., negative binomial, beta binomial, and/or rank based testing) and novel visualizations to improve interpretability (e.g., Rocky Mountain plots, longitudinal ordination plots). This comprehensive pipeline for microbiome analysis also maintains data structures familiar to R users to improve analysts’ control over workflow. A complete vignette is provided to aid new users in analysis workflow. Conclusions tidyMicro provides a reliable alternative to popular microbiome analysis packages in R. We provide standard tools as well as novel extensions on standard analyses to improve interpretability results while maintaining object malleability to encourage open source collaboration. The simple examples and full workflow from the package are reproducible and applicable to external data sets. |
| first_indexed | 2024-12-16T15:32:10Z |
| format | Article |
| id | doaj.art-a4a19f4ca003442b937182b3d0767559 |
| institution | Directory Open Access Journal |
| issn | 1471-2105 |
| language | English |
| last_indexed | 2024-12-16T15:32:10Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Bioinformatics |
| spelling | doaj.art-a4a19f4ca003442b937182b3d07675592022-12-21T22:26:18ZengBMCBMC Bioinformatics1471-21052021-02-0122111310.1186/s12859-021-03967-2tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in RCharlie M. Carpenter0Daniel N. Frank1Kayla Williamson2Jaron Arbet3Brandie D. Wagner4Katerina Kechris5Miranda E. Kroehl6Department of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical CampusDivision of Infectious Diseases, Department of Medicine, University of Colorado Anschutz Medical CampusDepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical CampusDepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical CampusDepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical CampusDepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical CampusDepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado Anschutz Medical CampusAbstract Background The drive to understand how microbial communities interact with their environments has inspired innovations across many fields. The data generated from sequence-based analyses of microbial communities typically are of high dimensionality and can involve multiple data tables consisting of taxonomic or functional gene/pathway counts. Merging multiple high dimensional tables with study-related metadata can be challenging. Existing microbiome pipelines available in R have created their own data structures to manage this problem. However, these data structures may be unfamiliar to analysts new to microbiome data or R and do not allow for deviations from internal workflows. Existing analysis tools also focus primarily on community-level analyses and exploratory visualizations, as opposed to analyses of individual taxa. Results We developed the R package “tidyMicro” to serve as a more complete microbiome analysis pipeline. This open source software provides all of the essential tools available in other popular packages (e.g., management of sequence count tables, standard exploratory visualizations, and diversity inference tools) supplemented with multiple options for regression modelling (e.g., negative binomial, beta binomial, and/or rank based testing) and novel visualizations to improve interpretability (e.g., Rocky Mountain plots, longitudinal ordination plots). This comprehensive pipeline for microbiome analysis also maintains data structures familiar to R users to improve analysts’ control over workflow. A complete vignette is provided to aid new users in analysis workflow. Conclusions tidyMicro provides a reliable alternative to popular microbiome analysis packages in R. We provide standard tools as well as novel extensions on standard analyses to improve interpretability results while maintaining object malleability to encourage open source collaboration. The simple examples and full workflow from the package are reproducible and applicable to external data sets.https://doi.org/10.1186/s12859-021-03967-2MicrobiomePipelineRTidyverseVisualizationOpen source |
| spellingShingle | Charlie M. Carpenter Daniel N. Frank Kayla Williamson Jaron Arbet Brandie D. Wagner Katerina Kechris Miranda E. Kroehl tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R BMC Bioinformatics Microbiome Pipeline R Tidyverse Visualization Open source |
| title | tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R |
| title_full | tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R |
| title_fullStr | tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R |
| title_full_unstemmed | tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R |
| title_short | tidyMicro: a pipeline for microbiome data analysis and visualization using the tidyverse in R |
| title_sort | tidymicro a pipeline for microbiome data analysis and visualization using the tidyverse in r |
| topic | Microbiome Pipeline R Tidyverse Visualization Open source |
| url | https://doi.org/10.1186/s12859-021-03967-2 |
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