Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages
<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that can cause infections in humans, especially in hospital patients with compromised host defence mechanisms, including patients with cystic fibrosis. Filamentous bacteriophages represent a group of single-stranded DNA viruses i...
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MDPI AG
2023-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/11/2215 |
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| author | Peter Evseev Julia Bocharova Dmitriy Shagin Igor Chebotar |
| author_facet | Peter Evseev Julia Bocharova Dmitriy Shagin Igor Chebotar |
| author_sort | Peter Evseev |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that can cause infections in humans, especially in hospital patients with compromised host defence mechanisms, including patients with cystic fibrosis. Filamentous bacteriophages represent a group of single-stranded DNA viruses infecting different bacteria, including <i>P. aeruginosa</i> and other human and animal pathogens; many of them can replicate when integrated into the bacterial chromosome. Filamentous bacteriophages can contribute to the virulence of <i>P. aeruginosa</i> and influence the course of the disease. There are just a few isolated and officially classified filamentous bacteriophages infecting <i>P. aeruginosa</i>, but genomic studies indicated the frequent occurrence of integrated prophages in many <i>P. aeruginosa</i> genomes. An analysis of sequenced genomes of <i>P. aeruginosa</i> isolated from upper respiratory tract (throat and nasal swabs) and sputum specimens collected from Russian patients with cystic fibrosis indicated a higher diversity of filamentous bacteriophages than first thought. A detailed analysis of predicted bacterial proteins revealed prophage regions representing the filamentous phages known to be quite distantly related to known phages. Genomic comparisons and phylogenetic studies enabled the proposal of several new taxonomic groups of filamentous bacteriophages. |
| first_indexed | 2024-03-09T16:22:54Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T16:22:54Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a4a3e9ebac9f49098666dc2d6a22778b2023-11-24T15:10:49ZengMDPI AGViruses1999-49152023-11-011511221510.3390/v15112215Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous BacteriophagesPeter Evseev0Julia Bocharova1Dmitriy Shagin2Igor Chebotar3Laboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, RussiaLaboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, RussiaLaboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, RussiaLaboratory of Molecular Microbiology, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, Russia<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that can cause infections in humans, especially in hospital patients with compromised host defence mechanisms, including patients with cystic fibrosis. Filamentous bacteriophages represent a group of single-stranded DNA viruses infecting different bacteria, including <i>P. aeruginosa</i> and other human and animal pathogens; many of them can replicate when integrated into the bacterial chromosome. Filamentous bacteriophages can contribute to the virulence of <i>P. aeruginosa</i> and influence the course of the disease. There are just a few isolated and officially classified filamentous bacteriophages infecting <i>P. aeruginosa</i>, but genomic studies indicated the frequent occurrence of integrated prophages in many <i>P. aeruginosa</i> genomes. An analysis of sequenced genomes of <i>P. aeruginosa</i> isolated from upper respiratory tract (throat and nasal swabs) and sputum specimens collected from Russian patients with cystic fibrosis indicated a higher diversity of filamentous bacteriophages than first thought. A detailed analysis of predicted bacterial proteins revealed prophage regions representing the filamentous phages known to be quite distantly related to known phages. Genomic comparisons and phylogenetic studies enabled the proposal of several new taxonomic groups of filamentous bacteriophages.https://www.mdpi.com/1999-4915/15/11/2215filamentous bacteriophagesPfPseudomonas phages<i>Pseudomonas aeruginosa</i>bacteriophage taxonomy |
| spellingShingle | Peter Evseev Julia Bocharova Dmitriy Shagin Igor Chebotar Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages Viruses filamentous bacteriophages Pf Pseudomonas phages <i>Pseudomonas aeruginosa</i> bacteriophage taxonomy |
| title | Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages |
| title_full | Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages |
| title_fullStr | Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages |
| title_full_unstemmed | Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages |
| title_short | Analysis of <i>Pseudomonas aeruginosa</i> Isolates from Patients with Cystic Fibrosis Revealed Novel Groups of Filamentous Bacteriophages |
| title_sort | analysis of i pseudomonas aeruginosa i isolates from patients with cystic fibrosis revealed novel groups of filamentous bacteriophages |
| topic | filamentous bacteriophages Pf Pseudomonas phages <i>Pseudomonas aeruginosa</i> bacteriophage taxonomy |
| url | https://www.mdpi.com/1999-4915/15/11/2215 |
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