Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria
The rise in bacterial resistance to currently used antibiotics is the main focus of medical researchers. Bacterial multidrug resistance (MDR) is a major threat to humans, as it is linked to greater rates of chronic disease and mortality. Hence, there is an urgent need for developing effective strate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/12/1469 |
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| author | Haseena Adnan Khan Iqra Ghaffar Roua S. Baty Mohamed M. Abdel-Daim Shahida M. Habib Tasmina Kanwal Muhammad Raza Shah |
| author_facet | Haseena Adnan Khan Iqra Ghaffar Roua S. Baty Mohamed M. Abdel-Daim Shahida M. Habib Tasmina Kanwal Muhammad Raza Shah |
| author_sort | Haseena |
| collection | DOAJ |
| description | The rise in bacterial resistance to currently used antibiotics is the main focus of medical researchers. Bacterial multidrug resistance (MDR) is a major threat to humans, as it is linked to greater rates of chronic disease and mortality. Hence, there is an urgent need for developing effective strategies to overcome the bacterial MDR. Metal–organic frameworks (MOFs) are a new class of porous crystalline materials made up of metal ions and organic ligands that can vary their pore size and structure to better encapsulate drug candidates. This study reports the synthesis of ribose-coated Cu-MOFs for enhanced bactericidal activity of chloramphenicol (CHL) against <i>Escherichia coli</i> (resistant and sensitive) and MDR <i>Pseudomonas aeruginosa</i>. The synthesized Cu-MOFs were characterized with DLS, FT-IR, powder X-ray diffraction, scanning electron microscope, and atomic force microscope. They were further investigated for their efficacy against selected bacterial strains. The synthesized ribose-coated Cu-MOFs were observed as spherical shape structure with the particle size of 562.84 ± 13.42 nm. CHL caused the increased inhibition of <i>E. coli</i> and MDR <i>P. aeruginosa</i> with significantly reduced MIC and MBIC values after being encapsulated in ribose-coated Cu-MOFs. The morphological analysis of the bacterial strains treated with ribose-coated CHL-Cu-MOFs showed the complete morphological distortion of both <i>E. coli</i> and MDR <i>P. aeruginosa.</i> Based on the results of the study, it can be suggested that ribose-coated Cu-MOFs may be an effective alternate candidate to overcome the MDR and provide new perspective for the treatment of MDR bacterial infections. |
| first_indexed | 2024-03-10T04:39:49Z |
| format | Article |
| id | doaj.art-a4ab6f491524456f83142130aae8073f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T04:39:49Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a4ab6f491524456f83142130aae8073f2023-11-23T03:30:19ZengMDPI AGAntibiotics2079-63822021-11-011012146910.3390/antibiotics10121469Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant BacteriaHaseena0Adnan Khan1Iqra Ghaffar2Roua S. Baty3Mohamed M. Abdel-Daim4Shahida M. Habib5Tasmina Kanwal6Muhammad Raza Shah7Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, PakistanInstitute of Chemical Sciences, University of Peshawar, Peshawar 25120, PakistanInternational Centre for Chemical and Biological Sciences, Research Institute of Chemistry, University of Karachi, Karachi 74200, PakistanDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaPharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptInternational Centre for Chemical and Biological Sciences, Research Institute of Chemistry, University of Karachi, Karachi 74200, PakistanInternational Centre for Chemical and Biological Sciences, Research Institute of Chemistry, University of Karachi, Karachi 74200, PakistanInternational Centre for Chemical and Biological Sciences, Research Institute of Chemistry, University of Karachi, Karachi 74200, PakistanThe rise in bacterial resistance to currently used antibiotics is the main focus of medical researchers. Bacterial multidrug resistance (MDR) is a major threat to humans, as it is linked to greater rates of chronic disease and mortality. Hence, there is an urgent need for developing effective strategies to overcome the bacterial MDR. Metal–organic frameworks (MOFs) are a new class of porous crystalline materials made up of metal ions and organic ligands that can vary their pore size and structure to better encapsulate drug candidates. This study reports the synthesis of ribose-coated Cu-MOFs for enhanced bactericidal activity of chloramphenicol (CHL) against <i>Escherichia coli</i> (resistant and sensitive) and MDR <i>Pseudomonas aeruginosa</i>. The synthesized Cu-MOFs were characterized with DLS, FT-IR, powder X-ray diffraction, scanning electron microscope, and atomic force microscope. They were further investigated for their efficacy against selected bacterial strains. The synthesized ribose-coated Cu-MOFs were observed as spherical shape structure with the particle size of 562.84 ± 13.42 nm. CHL caused the increased inhibition of <i>E. coli</i> and MDR <i>P. aeruginosa</i> with significantly reduced MIC and MBIC values after being encapsulated in ribose-coated Cu-MOFs. The morphological analysis of the bacterial strains treated with ribose-coated CHL-Cu-MOFs showed the complete morphological distortion of both <i>E. coli</i> and MDR <i>P. aeruginosa.</i> Based on the results of the study, it can be suggested that ribose-coated Cu-MOFs may be an effective alternate candidate to overcome the MDR and provide new perspective for the treatment of MDR bacterial infections.https://www.mdpi.com/2079-6382/10/12/1469multi-drug resistancemetal–organic frameworkchloramphenicolbiofilmantibiotic |
| spellingShingle | Haseena Adnan Khan Iqra Ghaffar Roua S. Baty Mohamed M. Abdel-Daim Shahida M. Habib Tasmina Kanwal Muhammad Raza Shah Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria Antibiotics multi-drug resistance metal–organic framework chloramphenicol biofilm antibiotic |
| title | Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria |
| title_full | Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria |
| title_fullStr | Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria |
| title_full_unstemmed | Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria |
| title_short | Synthesis of Ribose-Coated Copper-Based Metal–Organic Framework for Enhanced Antibacterial Potential of Chloramphenicol against Multi-Drug Resistant Bacteria |
| title_sort | synthesis of ribose coated copper based metal organic framework for enhanced antibacterial potential of chloramphenicol against multi drug resistant bacteria |
| topic | multi-drug resistance metal–organic framework chloramphenicol biofilm antibiotic |
| url | https://www.mdpi.com/2079-6382/10/12/1469 |
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