Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria
Abstract Background Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falci...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Malaria Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12936-020-03246-0 |
| _version_ | 1818980850810748928 |
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| author | Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga |
| author_facet | Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga |
| author_sort | Louis Regis Dossou-Yovo |
| collection | DOAJ |
| description | Abstract Background Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistance-1 gene (Pfmdr1) N86Y mutation is associated with reduced susceptibility to lumefantrine. This study assessed the prevalence of Pfmdr1 N86Y in Brazzaville, Republic of Congo. Methods A total 1001 of P. falciparum-infected blood samples obtained from asymptomatic malaria pregnant women having a normal child delivery at the Madibou Integrated Health Centre were analysed. Pfmdr1 N86Y genotyping was conducted using PCR-restriction fragment length polymorphism. Results The wild type Pfmdr1 N86 allele was predominant (> 68%) in this study, whereas a few isolates carrying the either the mutant allele (Pfmdr1 86Y) alone or both alleles (mixed genotype). The dominance of the wildtype allele (pfmdr1 N86) indicates the plausible decline P. falciparum susceptibility to lumefantrine. Conclusion This study gives an update on the prevalence of Pfmdr1 N86Y alleles in Brazzaville, Republic of Congo. It also raises concern on the imminent emergence of resistance against artemether–lumefantrine in this setting. This study underscores the importance to regular artemether–lumefantrine efficacy monitoring to inform the malaria control programme of the Republic of Congo. |
| first_indexed | 2024-12-20T17:21:59Z |
| format | Article |
| id | doaj.art-a4ac3c2a27fb40daa4766a8f8ab04d66 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-20T17:21:59Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-a4ac3c2a27fb40daa4766a8f8ab04d662022-12-21T19:31:46ZengBMCMalaria Journal1475-28752020-05-011911610.1186/s12936-020-03246-0Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malariaLouis Regis Dossou-Yovo0Francine Ntoumi1Felix Koukouikila-Koussounda2Jeannhey Christevy Vouvoungui3Ayodele Adedoja4David Nderu5Thirumalaisamy P. Velavan6Arsène Lenga7Ecole Normale Supérieure, Marien Ngouabi UniversityCongolese Foundation for Medical ResearchCongolese Foundation for Medical ResearchCongolese Foundation for Medical ResearchCongolese Foundation for Medical ResearchSchool of Health Sciences, Kirinyaga UniversityInstitute of Tropical Medicine, University of TübingenFaculty of Science and Technology, Marien Ngouabi UniversityAbstract Background Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistance-1 gene (Pfmdr1) N86Y mutation is associated with reduced susceptibility to lumefantrine. This study assessed the prevalence of Pfmdr1 N86Y in Brazzaville, Republic of Congo. Methods A total 1001 of P. falciparum-infected blood samples obtained from asymptomatic malaria pregnant women having a normal child delivery at the Madibou Integrated Health Centre were analysed. Pfmdr1 N86Y genotyping was conducted using PCR-restriction fragment length polymorphism. Results The wild type Pfmdr1 N86 allele was predominant (> 68%) in this study, whereas a few isolates carrying the either the mutant allele (Pfmdr1 86Y) alone or both alleles (mixed genotype). The dominance of the wildtype allele (pfmdr1 N86) indicates the plausible decline P. falciparum susceptibility to lumefantrine. Conclusion This study gives an update on the prevalence of Pfmdr1 N86Y alleles in Brazzaville, Republic of Congo. It also raises concern on the imminent emergence of resistance against artemether–lumefantrine in this setting. This study underscores the importance to regular artemether–lumefantrine efficacy monitoring to inform the malaria control programme of the Republic of Congo.http://link.springer.com/article/10.1186/s12936-020-03246-0Plasmodium falciparumPfmdr1Antimalarial drug resistanceLumefantrineBrazzavilleRepublic of Congo |
| spellingShingle | Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria Malaria Journal Plasmodium falciparum Pfmdr1 Antimalarial drug resistance Lumefantrine Brazzaville Republic of Congo |
| title | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
| title_full | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
| title_fullStr | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
| title_full_unstemmed | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
| title_short | Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
| title_sort | molecular surveillance of the pfmdr1 n86y allele among congolese pregnant women with asymptomatic malaria |
| topic | Plasmodium falciparum Pfmdr1 Antimalarial drug resistance Lumefantrine Brazzaville Republic of Congo |
| url | http://link.springer.com/article/10.1186/s12936-020-03246-0 |
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