Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces
Although biofilm formation is a very effective mechanism to sustain bacterial life, it is detrimental in medical and industrial sectors. Current strategies to control biofilm proliferation are typically based on biocides, which exhibit a negative environmental impact. In the search for environmental...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/9/1/49 |
| _version_ | 1818571647433572352 |
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| author | Elsie Zurob Geraldine Dennett Dana Gentil Francisco Montero-Silva Ulrike Gerber Pamela Naulín Andrea Gómez Raúl Fuentes Sheila Lascano Thiago Henrique Rodrigues da Cunha Cristian Ramírez Ricardo Henríquez Valeria del Campo Nelson Barrera Marcela Wilkens Carolina Parra |
| author_facet | Elsie Zurob Geraldine Dennett Dana Gentil Francisco Montero-Silva Ulrike Gerber Pamela Naulín Andrea Gómez Raúl Fuentes Sheila Lascano Thiago Henrique Rodrigues da Cunha Cristian Ramírez Ricardo Henríquez Valeria del Campo Nelson Barrera Marcela Wilkens Carolina Parra |
| author_sort | Elsie Zurob |
| collection | DOAJ |
| description | Although biofilm formation is a very effective mechanism to sustain bacterial life, it is detrimental in medical and industrial sectors. Current strategies to control biofilm proliferation are typically based on biocides, which exhibit a negative environmental impact. In the search for environmentally friendly solutions, nanotechnology opens the possibility to control the interaction between biological systems and colonized surfaces by introducing nanostructured coatings that have the potential to affect bacterial adhesion by modifying surface properties at the same scale. In this work, we present a study on the performance of graphene and hexagonal boron nitride coatings (h-BN) to reduce biofilm formation. In contraposition to planktonic state, we focused on evaluating the efficiency of graphene and h-BN at the irreversible stage of biofilm formation, where most of the biocide solutions have a poor performance. A wild Enterobacter cloacae strain was isolated, from fouling found in a natural environment, and used in these experiments. According to our results, graphene and h-BN coatings modify surface energy and electrostatic interactions with biological systems. This nanoscale modification determines a significant reduction in biofilm formation at its irreversible stage. No bactericidal effects were found, suggesting both coatings offer a biocompatible solution for biofilm and fouling control in a wide range of applications. |
| first_indexed | 2024-12-14T13:58:48Z |
| format | Article |
| id | doaj.art-a4ad3e2015a64fb094ac3f2a3109c794 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-14T13:58:48Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-a4ad3e2015a64fb094ac3f2a3109c7942022-12-21T22:58:46ZengMDPI AGNanomaterials2079-49912019-01-01914910.3390/nano9010049nano9010049Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated SurfacesElsie Zurob0Geraldine Dennett1Dana Gentil2Francisco Montero-Silva3Ulrike Gerber4Pamela Naulín5Andrea Gómez6Raúl Fuentes7Sheila Lascano8Thiago Henrique Rodrigues da Cunha9Cristian Ramírez10Ricardo Henríquez11Valeria del Campo12Nelson Barrera13Marcela Wilkens14Carolina Parra15Laboratorio Nanobiomateriales, Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileLaboratorio Nanobiomateriales, Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileLaboratorio Nanobiomateriales, Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileLaboratorio Nanobiomateriales, Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileFaculty Environment and Natural Science, Institute of Biotechnology, Brandenburg University of Technology, Universitätsplatz 1, 01968 Senftenberg, GermanyFacultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, ChileFacultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, ChileDepartamento de Industrias, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileDepartamento de Mecánica, Universidad Técnica Federico Santa María, Avda. Vicuña Mackenna 3939, Santiago, ChileDepartamento de Física, CTNanotubos, Universidade Federal de Minas Gerais, Belo Horizonte 31310260, BrazilDepartamento de Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileDepartamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileDepartamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileFacultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, ChileLaboratorio de Microbiología Básica y Aplicada, Universidad de Santiago de Chile, Avenida Libertador Bernardo O’Higgins 3363, Santiago, ChileLaboratorio Nanobiomateriales, Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, ChileAlthough biofilm formation is a very effective mechanism to sustain bacterial life, it is detrimental in medical and industrial sectors. Current strategies to control biofilm proliferation are typically based on biocides, which exhibit a negative environmental impact. In the search for environmentally friendly solutions, nanotechnology opens the possibility to control the interaction between biological systems and colonized surfaces by introducing nanostructured coatings that have the potential to affect bacterial adhesion by modifying surface properties at the same scale. In this work, we present a study on the performance of graphene and hexagonal boron nitride coatings (h-BN) to reduce biofilm formation. In contraposition to planktonic state, we focused on evaluating the efficiency of graphene and h-BN at the irreversible stage of biofilm formation, where most of the biocide solutions have a poor performance. A wild Enterobacter cloacae strain was isolated, from fouling found in a natural environment, and used in these experiments. According to our results, graphene and h-BN coatings modify surface energy and electrostatic interactions with biological systems. This nanoscale modification determines a significant reduction in biofilm formation at its irreversible stage. No bactericidal effects were found, suggesting both coatings offer a biocompatible solution for biofilm and fouling control in a wide range of applications.http://www.mdpi.com/2079-4991/9/1/49grapheneh-BNnanostructured coatingsbiofilmsE. cloacae |
| spellingShingle | Elsie Zurob Geraldine Dennett Dana Gentil Francisco Montero-Silva Ulrike Gerber Pamela Naulín Andrea Gómez Raúl Fuentes Sheila Lascano Thiago Henrique Rodrigues da Cunha Cristian Ramírez Ricardo Henríquez Valeria del Campo Nelson Barrera Marcela Wilkens Carolina Parra Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces Nanomaterials graphene h-BN nanostructured coatings biofilms E. cloacae |
| title | Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces |
| title_full | Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces |
| title_fullStr | Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces |
| title_full_unstemmed | Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces |
| title_short | Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces |
| title_sort | inhibition of wild enterobacter cloacae biofilm formation by nanostructured graphene and hexagonal boron nitride coated surfaces |
| topic | graphene h-BN nanostructured coatings biofilms E. cloacae |
| url | http://www.mdpi.com/2079-4991/9/1/49 |
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