Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium
Polyester plastics are widely used in daily life, but also cause a large amount of waste. Degradation by microbial enzymes is the most promising way for the biobased upcycling of the wastes. However, there is still a shortage of high-performance enzymes, and more efficient polyester hydrolases need...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1349010/full |
| _version_ | 1827350900777156608 |
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| author | Xiaoli Zhou Xianmin Zhou Zhiqiang Xu Mingxia Zhang Honghui Zhu |
| author_facet | Xiaoli Zhou Xianmin Zhou Zhiqiang Xu Mingxia Zhang Honghui Zhu |
| author_sort | Xiaoli Zhou |
| collection | DOAJ |
| description | Polyester plastics are widely used in daily life, but also cause a large amount of waste. Degradation by microbial enzymes is the most promising way for the biobased upcycling of the wastes. However, there is still a shortage of high-performance enzymes, and more efficient polyester hydrolases need to be developed. Here we identified two polyester hydrolases, jmPE13 and jmPE14, from a previously isolated strain Pseudomonas sp. JM16B3. The proteins were recombinantly expressed and purified in E. coli, and their enzymatic properties were characterized. JmPE13 and jmPE14 showed hydrolytic activity towards polyethylene terephthalate (PET) and Poly (butylene adipate-co-terephthalate) (PBAT) at medium temperatures. The enzyme activity and stability of jmPE13 were further improved to 3- and 1.5-fold, respectively, by rational design. The results of our research can be helpful for further engineering of more efficient polyester plastic hydrolases and their industrial applications. |
| first_indexed | 2024-03-08T01:48:16Z |
| format | Article |
| id | doaj.art-a4af4d3ace1e4e67a0013657741d7026 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T01:48:16Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a4af4d3ace1e4e67a0013657741d70262024-02-14T12:18:43ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-02-011210.3389/fbioe.2024.13490101349010Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacteriumXiaoli ZhouXianmin ZhouZhiqiang XuMingxia ZhangHonghui ZhuPolyester plastics are widely used in daily life, but also cause a large amount of waste. Degradation by microbial enzymes is the most promising way for the biobased upcycling of the wastes. However, there is still a shortage of high-performance enzymes, and more efficient polyester hydrolases need to be developed. Here we identified two polyester hydrolases, jmPE13 and jmPE14, from a previously isolated strain Pseudomonas sp. JM16B3. The proteins were recombinantly expressed and purified in E. coli, and their enzymatic properties were characterized. JmPE13 and jmPE14 showed hydrolytic activity towards polyethylene terephthalate (PET) and Poly (butylene adipate-co-terephthalate) (PBAT) at medium temperatures. The enzyme activity and stability of jmPE13 were further improved to 3- and 1.5-fold, respectively, by rational design. The results of our research can be helpful for further engineering of more efficient polyester plastic hydrolases and their industrial applications.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1349010/fullpolyesterhydrolaseenzyme engineeringPETPBAT |
| spellingShingle | Xiaoli Zhou Xianmin Zhou Zhiqiang Xu Mingxia Zhang Honghui Zhu Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium Frontiers in Bioengineering and Biotechnology polyester hydrolase enzyme engineering PET PBAT |
| title | Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium |
| title_full | Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium |
| title_fullStr | Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium |
| title_full_unstemmed | Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium |
| title_short | Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium |
| title_sort | characterization and engineering of plastic degrading polyesterases jmpe13 and jmpe14 from pseudomonas bacterium |
| topic | polyester hydrolase enzyme engineering PET PBAT |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1349010/full |
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