RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae)
<i>Empoasca onukii</i> Matsuda is a primary pest of the tea plant <i>Camellia sinensis</i> (L.) O. Ktze that severely influences the production and quality of tea products. Gustatory receptors (GRs) are an indispensable part of the <i>E. onukii</i> chemosensory ma...
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MDPI AG
2023-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/9/2221 |
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| author | Ruirui Zhang Xiaoyue Lun Yunhe Zhao Yu Zhang Yan Cao Xiangzhi Zhang Meina Jin Zhengqun Zhang Xiuxiu Xu |
| author_facet | Ruirui Zhang Xiaoyue Lun Yunhe Zhao Yu Zhang Yan Cao Xiangzhi Zhang Meina Jin Zhengqun Zhang Xiuxiu Xu |
| author_sort | Ruirui Zhang |
| collection | DOAJ |
| description | <i>Empoasca onukii</i> Matsuda is a primary pest of the tea plant <i>Camellia sinensis</i> (L.) O. Ktze that severely influences the production and quality of tea products. Gustatory receptors (GRs) are an indispensable part of the <i>E. onukii</i> chemosensory machinery as members of the G-protein coupled receptor family. Insect odor and gustatory receptors are consumingly sensitive and selective sensory receptors to search for foraging, mates, and spawning sites. In this study, the gustatory receptor <i>EonuGR1</i> was cloned and analyzed bioinformatically, and the expression levels of <i>EonuGR1</i> in diverse tissues of <i>E. onukii</i> were tested via qRT-PCR. The behavioral response of <i>E. onukii</i> to volatile compounds was determined via RNA interference and Y-tube olfactometer assays to investigate the role of <i>EonuGR1</i> in the olfactory recognition of <i>E. onukii</i>. The coding sequence length of <i>EonuGR1</i> was 1062 bp, and the length of the protein encoded by <i>EonuGR1</i> was 40.52 kD. The highest interference efficiency was observed after 3 h of dsEonuGR1 treatment via root soak treatment. Moreover, the response rates to phenylacetaldehyde at concentrations of 10 and 0.1 µL/mL were significantly downregulated in <i>E. onukii</i>. The responses to phenylacetaldehyde at concentrations of 10 and 100 µL/mL showed a significant decrease after dsEonuGR1 treatment for 12 h in <i>E. onukii</i>. In conclusion, <i>EonuGR1</i> was highly expressed in the abdomen and functioned in olfactory recognition of the tea plant volatile phenylacetaldehyde by <i>E. onukii</i>. Overall, <i>EonuGR1</i> has the potential as a gene target for the design of effective control strategies against <i>E. onukii</i>. |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T23:08:45Z |
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| spelling | doaj.art-a4affae5731e4b3ab2a9cc43f5fc4c7d2023-11-19T09:09:15ZengMDPI AGAgronomy2073-43952023-08-01139222110.3390/agronomy13092221RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae)Ruirui Zhang0Xiaoyue Lun1Yunhe Zhao2Yu Zhang3Yan Cao4Xiangzhi Zhang5Meina Jin6Zhengqun Zhang7Xiuxiu Xu8Tea Research Institute, Shandong Academy of Agricultural Science, Jinan 250100, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaTea Research Institute, Shandong Academy of Agricultural Science, Jinan 250100, China<i>Empoasca onukii</i> Matsuda is a primary pest of the tea plant <i>Camellia sinensis</i> (L.) O. Ktze that severely influences the production and quality of tea products. Gustatory receptors (GRs) are an indispensable part of the <i>E. onukii</i> chemosensory machinery as members of the G-protein coupled receptor family. Insect odor and gustatory receptors are consumingly sensitive and selective sensory receptors to search for foraging, mates, and spawning sites. In this study, the gustatory receptor <i>EonuGR1</i> was cloned and analyzed bioinformatically, and the expression levels of <i>EonuGR1</i> in diverse tissues of <i>E. onukii</i> were tested via qRT-PCR. The behavioral response of <i>E. onukii</i> to volatile compounds was determined via RNA interference and Y-tube olfactometer assays to investigate the role of <i>EonuGR1</i> in the olfactory recognition of <i>E. onukii</i>. The coding sequence length of <i>EonuGR1</i> was 1062 bp, and the length of the protein encoded by <i>EonuGR1</i> was 40.52 kD. The highest interference efficiency was observed after 3 h of dsEonuGR1 treatment via root soak treatment. Moreover, the response rates to phenylacetaldehyde at concentrations of 10 and 0.1 µL/mL were significantly downregulated in <i>E. onukii</i>. The responses to phenylacetaldehyde at concentrations of 10 and 100 µL/mL showed a significant decrease after dsEonuGR1 treatment for 12 h in <i>E. onukii</i>. In conclusion, <i>EonuGR1</i> was highly expressed in the abdomen and functioned in olfactory recognition of the tea plant volatile phenylacetaldehyde by <i>E. onukii</i>. Overall, <i>EonuGR1</i> has the potential as a gene target for the design of effective control strategies against <i>E. onukii</i>.https://www.mdpi.com/2073-4395/13/9/2221<i>Empoasca onukii</i> Matsuda<i>EonuGR1</i>RNA interferencetea plant volatiles |
| spellingShingle | Ruirui Zhang Xiaoyue Lun Yunhe Zhao Yu Zhang Yan Cao Xiangzhi Zhang Meina Jin Zhengqun Zhang Xiuxiu Xu RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae) Agronomy <i>Empoasca onukii</i> Matsuda <i>EonuGR1</i> RNA interference tea plant volatiles |
| title | RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae) |
| title_full | RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae) |
| title_fullStr | RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae) |
| title_full_unstemmed | RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae) |
| title_short | RNAi-Mediated Interference with <i>EonuGR1</i> Affects the Recognition of Phenylacetaldehyde by <i>Empoasca onukii</i> Matsuda (Hemiptera: Cicadellidae) |
| title_sort | rnai mediated interference with i eonugr1 i affects the recognition of phenylacetaldehyde by i empoasca onukii i matsuda hemiptera cicadellidae |
| topic | <i>Empoasca onukii</i> Matsuda <i>EonuGR1</i> RNA interference tea plant volatiles |
| url | https://www.mdpi.com/2073-4395/13/9/2221 |
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