Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium
Preparation of paraspinal tissue of patients with implants for elemental analysis is a challenge because it contains titanium in the ionic form, as well as metallic debris. Most literature reports focus on dissolving the tissue, but the impact of digestion conditions on metallic debris of Ti has not...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/8/2120 |
| _version_ | 1797538553053315072 |
|---|---|
| author | Jan Sawicki Anna Danielewicz Magdalena Wójciak Michał Latalski Agnieszka Skalska-Kamińska Katarzyna Tyszczuk-Rotko Ireneusz Sowa |
| author_facet | Jan Sawicki Anna Danielewicz Magdalena Wójciak Michał Latalski Agnieszka Skalska-Kamińska Katarzyna Tyszczuk-Rotko Ireneusz Sowa |
| author_sort | Jan Sawicki |
| collection | DOAJ |
| description | Preparation of paraspinal tissue of patients with implants for elemental analysis is a challenge because it contains titanium in the ionic form, as well as metallic debris. Most literature reports focus on dissolving the tissue, but the impact of digestion conditions on metallic debris of Ti has not been investigated. In our work, various digestion conditions, including systems, compositions of oxidising mixture, and time, were tested aiming (i) to digest the tissue without digestion of metallic titanium to quantify soluble Ti and (ii) to digest metallic titanium debris to asses total Ti content in tissue. The experiments were performed in a closed mode using a microwave-assisted system and a carbon heating block. Our study revealed that total digestion of titanium was impossible in the tested conditions and the maximal level of digested titanium was below 70%. The mineralisation with the use of concentrated nitric acid was optimal to prepare paraspinal samples to analyse the soluble titanium form because metallic titanium passivated and did not migrate to the solution. The elaborated conditions were applied to determine titanium ion in the periimplant tissue of patients with three different titanium-based surgical systems, including traditional growing rod (TGR), guided growth systems (GGS), and vertical expandable prosthesis titanium rib (VEPTR). |
| first_indexed | 2024-03-10T12:33:03Z |
| format | Article |
| id | doaj.art-a4b072c01225471a908aec988bbfa3c1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:33:03Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a4b072c01225471a908aec988bbfa3c12023-11-21T14:32:27ZengMDPI AGMolecules1420-30492021-04-01268212010.3390/molecules26082120Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of TitaniumJan Sawicki0Anna Danielewicz1Magdalena Wójciak2Michał Latalski3Agnieszka Skalska-Kamińska4Katarzyna Tyszczuk-Rotko5Ireneusz Sowa6Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Paediatric Orthopaedics, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Paediatric Orthopaedics, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandFaculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University in Lublin, 20-031 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandPreparation of paraspinal tissue of patients with implants for elemental analysis is a challenge because it contains titanium in the ionic form, as well as metallic debris. Most literature reports focus on dissolving the tissue, but the impact of digestion conditions on metallic debris of Ti has not been investigated. In our work, various digestion conditions, including systems, compositions of oxidising mixture, and time, were tested aiming (i) to digest the tissue without digestion of metallic titanium to quantify soluble Ti and (ii) to digest metallic titanium debris to asses total Ti content in tissue. The experiments were performed in a closed mode using a microwave-assisted system and a carbon heating block. Our study revealed that total digestion of titanium was impossible in the tested conditions and the maximal level of digested titanium was below 70%. The mineralisation with the use of concentrated nitric acid was optimal to prepare paraspinal samples to analyse the soluble titanium form because metallic titanium passivated and did not migrate to the solution. The elaborated conditions were applied to determine titanium ion in the periimplant tissue of patients with three different titanium-based surgical systems, including traditional growing rod (TGR), guided growth systems (GGS), and vertical expandable prosthesis titanium rib (VEPTR).https://www.mdpi.com/1420-3049/26/8/2120titanium implantsoptimization of digestionparaspinal tissuemicrowave mineralizationcarbon heating block |
| spellingShingle | Jan Sawicki Anna Danielewicz Magdalena Wójciak Michał Latalski Agnieszka Skalska-Kamińska Katarzyna Tyszczuk-Rotko Ireneusz Sowa Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium Molecules titanium implants optimization of digestion paraspinal tissue microwave mineralization carbon heating block |
| title | Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium |
| title_full | Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium |
| title_fullStr | Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium |
| title_full_unstemmed | Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium |
| title_short | Analytical Problems with Preparation of Paraspinal Tissues from Patients with Spinal Fusion for Analysis of Titanium |
| title_sort | analytical problems with preparation of paraspinal tissues from patients with spinal fusion for analysis of titanium |
| topic | titanium implants optimization of digestion paraspinal tissue microwave mineralization carbon heating block |
| url | https://www.mdpi.com/1420-3049/26/8/2120 |
| work_keys_str_mv | AT jansawicki analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium AT annadanielewicz analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium AT magdalenawojciak analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium AT michałlatalski analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium AT agnieszkaskalskakaminska analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium AT katarzynatyszczukrotko analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium AT ireneuszsowa analyticalproblemswithpreparationofparaspinaltissuesfrompatientswithspinalfusionforanalysisoftitanium |