In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune
Abstract The emergence of antibiotic resistance in pathogens is one of the major health concerns facing mankind as different bacterial strains have developed resistance to antibiotics over the period of time due to overuse and misuse of antibiotics. Besides this, ability to form biofilms is another...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-01-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-024-01663-x |
| _version_ | 1797349665104986112 |
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| author | Avinash Sharma Muzamil Rashid Pooja Chauhan Sukhraj Kaur Amarjeet Kaur |
| author_facet | Avinash Sharma Muzamil Rashid Pooja Chauhan Sukhraj Kaur Amarjeet Kaur |
| author_sort | Avinash Sharma |
| collection | DOAJ |
| description | Abstract The emergence of antibiotic resistance in pathogens is one of the major health concerns facing mankind as different bacterial strains have developed resistance to antibiotics over the period of time due to overuse and misuse of antibiotics. Besides this, ability to form biofilms is another major factor contributing to antibiotic resistance, which has necessitated the need for exploration for novel and effective compounds with ability to inhibit biofilm formation. Endophytic fungi are reported to exhibit antibacterial and anti-biofilm potential and could serve as a potent source of novel antibacterial compounds. Majority of the bioactivities have been reported from fungi belonging to phylum Ascomycota. Endophytic basidiomycetes, inspite of their profound ability to serve as a source of bioactive compounds have not been exploited extensively. In present study, an attempt was made to assess the antibacterial, anti-biofilm and biofilm dispersion potential of an endophytic basidiomycetous fungus Schizophyllum commune procured from the culture collection of our lab. Ethyl acetate extract of S. commune showed good antibacterial activity against Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica and Vibrio cholerae. Minimum inhibitory concentration and minimum bactericidal concentration of the extract were in the range of 1.25-10 mg/ml against the tested bacterial pathogens. The mode of action was determined to be bactericidal which was further confirmed by time kill studies. Good anti-biofilm activity of S. commune extract was recorded against K. pneumoniae and S. enterica, which was further validated by fluorescence microscopy. The present study highlights the importance of endophytic basidiomycetes as source of therapeutic compounds. |
| first_indexed | 2024-03-08T12:33:40Z |
| format | Article |
| id | doaj.art-a4b23ecaf063495cb6d4624062a96a22 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-03-08T12:33:40Z |
| publishDate | 2024-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-a4b23ecaf063495cb6d4624062a96a222024-01-21T12:36:44ZengSpringerOpenAMB Express2191-08552024-01-0114111410.1186/s13568-024-01663-xIn vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum communeAvinash Sharma0Muzamil Rashid1Pooja Chauhan2Sukhraj Kaur3Amarjeet Kaur4Department of Microbiology, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityDepartment of Microbiology, Guru Nanak Dev UniversityAbstract The emergence of antibiotic resistance in pathogens is one of the major health concerns facing mankind as different bacterial strains have developed resistance to antibiotics over the period of time due to overuse and misuse of antibiotics. Besides this, ability to form biofilms is another major factor contributing to antibiotic resistance, which has necessitated the need for exploration for novel and effective compounds with ability to inhibit biofilm formation. Endophytic fungi are reported to exhibit antibacterial and anti-biofilm potential and could serve as a potent source of novel antibacterial compounds. Majority of the bioactivities have been reported from fungi belonging to phylum Ascomycota. Endophytic basidiomycetes, inspite of their profound ability to serve as a source of bioactive compounds have not been exploited extensively. In present study, an attempt was made to assess the antibacterial, anti-biofilm and biofilm dispersion potential of an endophytic basidiomycetous fungus Schizophyllum commune procured from the culture collection of our lab. Ethyl acetate extract of S. commune showed good antibacterial activity against Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica and Vibrio cholerae. Minimum inhibitory concentration and minimum bactericidal concentration of the extract were in the range of 1.25-10 mg/ml against the tested bacterial pathogens. The mode of action was determined to be bactericidal which was further confirmed by time kill studies. Good anti-biofilm activity of S. commune extract was recorded against K. pneumoniae and S. enterica, which was further validated by fluorescence microscopy. The present study highlights the importance of endophytic basidiomycetes as source of therapeutic compounds.https://doi.org/10.1186/s13568-024-01663-xSchizophyllum communeBasidiomycetesAntibacterialAnti-biofilmEndophyte |
| spellingShingle | Avinash Sharma Muzamil Rashid Pooja Chauhan Sukhraj Kaur Amarjeet Kaur In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune AMB Express Schizophyllum commune Basidiomycetes Antibacterial Anti-biofilm Endophyte |
| title | In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune |
| title_full | In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune |
| title_fullStr | In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune |
| title_full_unstemmed | In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune |
| title_short | In vitro antibacterial and anti-biofilm potential of an endophytic Schizophyllum commune |
| title_sort | in vitro antibacterial and anti biofilm potential of an endophytic schizophyllum commune |
| topic | Schizophyllum commune Basidiomycetes Antibacterial Anti-biofilm Endophyte |
| url | https://doi.org/10.1186/s13568-024-01663-x |
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