Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells
This study aims to investigate the effects of a novel ZnCuO nanoparticle coating for dental implants—versus those of conventional titanium surfaces—on bacteria and host cells. A multispecies biofilm composed of <i>Streptococcus sanguinis</i>, <i>Actinomyces naeslundii</i>, &l...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/7/2514 |
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| author | Kamal Dabbah Ilana Perelshtein Aharon Gedanken Yael Houri-Haddad Osnat Feuerstein |
| author_facet | Kamal Dabbah Ilana Perelshtein Aharon Gedanken Yael Houri-Haddad Osnat Feuerstein |
| author_sort | Kamal Dabbah |
| collection | DOAJ |
| description | This study aims to investigate the effects of a novel ZnCuO nanoparticle coating for dental implants—versus those of conventional titanium surfaces—on bacteria and host cells. A multispecies biofilm composed of <i>Streptococcus sanguinis</i>, <i>Actinomyces naeslundii</i>, <i>Porphyromonas gingivalis</i>, and <i>Fusobacterium nucleatum</i> was grown for 14 days on various titanium discs: machined, sandblasted, sandblasted and acid-etched (SLA), ZnCuO-coated, and hydroxyapatite discs. Bacterial species were quantified with qPCR, and their viability was examined via confocal microscopy. Osteoblast-like and macrophage-like cells grown on the various discs for 48 h were examined for proliferation using an XTT assay, and for activity using ALP and TNF-α assays. The CSLM revealed more dead bacteria in biofilms grown on titanium than on hydroxyapatite, and less on sandblasted than on machined and ZnCuO-coated surfaces, with the latter showing a significant decrease in all four biofilm species. The osteoblast-like cells showed increased proliferation on all of the titanium surfaces, with higher activity on the ZnCuO-coated and sandblasted discs. The macrophage-like cells showed higher proliferation on the hydroxyapatite and sandblasted discs, and lower activity on the SLA and ZnCuO-coated discs. The ZnCuO-coated titanium has anti-biofilm characteristics with desired effects on host cells, thus representing a promising candidate in the complex battle against peri-implantitis. |
| first_indexed | 2024-03-09T11:40:29Z |
| format | Article |
| id | doaj.art-a4b6673d58314e0fa92687117c116a78 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T11:40:29Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-a4b6673d58314e0fa92687117c116a782023-11-30T23:33:11ZengMDPI AGMaterials1996-19442022-03-01157251410.3390/ma15072514Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host CellsKamal Dabbah0Ilana Perelshtein1Aharon Gedanken2Yael Houri-Haddad3Osnat Feuerstein4Department of Prosthodontics, Hadassah Medical Center, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, IsraelDepartment of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 5290002, IsraelDepartment of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 5290002, IsraelDepartment of Prosthodontics, Hadassah Medical Center, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, IsraelDepartment of Prosthodontics, Hadassah Medical Center, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, IsraelThis study aims to investigate the effects of a novel ZnCuO nanoparticle coating for dental implants—versus those of conventional titanium surfaces—on bacteria and host cells. A multispecies biofilm composed of <i>Streptococcus sanguinis</i>, <i>Actinomyces naeslundii</i>, <i>Porphyromonas gingivalis</i>, and <i>Fusobacterium nucleatum</i> was grown for 14 days on various titanium discs: machined, sandblasted, sandblasted and acid-etched (SLA), ZnCuO-coated, and hydroxyapatite discs. Bacterial species were quantified with qPCR, and their viability was examined via confocal microscopy. Osteoblast-like and macrophage-like cells grown on the various discs for 48 h were examined for proliferation using an XTT assay, and for activity using ALP and TNF-α assays. The CSLM revealed more dead bacteria in biofilms grown on titanium than on hydroxyapatite, and less on sandblasted than on machined and ZnCuO-coated surfaces, with the latter showing a significant decrease in all four biofilm species. The osteoblast-like cells showed increased proliferation on all of the titanium surfaces, with higher activity on the ZnCuO-coated and sandblasted discs. The macrophage-like cells showed higher proliferation on the hydroxyapatite and sandblasted discs, and lower activity on the SLA and ZnCuO-coated discs. The ZnCuO-coated titanium has anti-biofilm characteristics with desired effects on host cells, thus representing a promising candidate in the complex battle against peri-implantitis.https://www.mdpi.com/1996-1944/15/7/2514antimicrobials/antimicrobial resistancebiofilm(s)dental implant(s)immunityosteoblast(s)macrophage(s) |
| spellingShingle | Kamal Dabbah Ilana Perelshtein Aharon Gedanken Yael Houri-Haddad Osnat Feuerstein Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells Materials antimicrobials/antimicrobial resistance biofilm(s) dental implant(s) immunity osteoblast(s) macrophage(s) |
| title | Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells |
| title_full | Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells |
| title_fullStr | Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells |
| title_full_unstemmed | Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells |
| title_short | Effects of a ZnCuO-Nanocoated Ti-6Al-4V Surface on Bacterial and Host Cells |
| title_sort | effects of a zncuo nanocoated ti 6al 4v surface on bacterial and host cells |
| topic | antimicrobials/antimicrobial resistance biofilm(s) dental implant(s) immunity osteoblast(s) macrophage(s) |
| url | https://www.mdpi.com/1996-1944/15/7/2514 |
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