In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste
Waste generated from the processing of marine organisms for food represents an underutilized resource that has the potential to provide bioactive molecules with pharmaceutical applications. Some of these molecules have known anti-thrombotic and anti-coagulant activities and are being investigated as...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-12-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/15/1/8 |
| _version_ | 1811264091216412672 |
|---|---|
| author | Hafiz Ansar Rasul Suleria Barney M. Hines Rama Addepalli Wei Chen Paul Masci Glenda Gobe Simone A. Osborne |
| author_facet | Hafiz Ansar Rasul Suleria Barney M. Hines Rama Addepalli Wei Chen Paul Masci Glenda Gobe Simone A. Osborne |
| author_sort | Hafiz Ansar Rasul Suleria |
| collection | DOAJ |
| description | Waste generated from the processing of marine organisms for food represents an underutilized resource that has the potential to provide bioactive molecules with pharmaceutical applications. Some of these molecules have known anti-thrombotic and anti-coagulant activities and are being investigated as alternatives to common anti-thrombotic drugs, like heparin and warfarin that have serious side effects. In the current study, extracts prepared from blacklip abalone (Haliotis rubra) processing waste, using food grade enzymes papain and bromelain, were found to contain sulphated polysaccharide with anti-thrombotic activity. Extracts were found to be enriched with sulphated polysaccharides and assessed for anti-thrombotic activity in vitro through heparin cofactor-II (HCII)-mediated inhibition of thrombin. More than 60% thrombin inhibition was observed in response to 100 μg/mL sulphated polysaccharides. Anti-thrombotic potential was further assessed as anti-coagulant activity in plasma and blood, using prothrombin time (PT), activated partial thromboplastin time (aPTT), and thromboelastography (TEG). All abalone extracts had significant activity compared with saline control. Anion exchange chromatography was used to separate extracts into fractions with enhanced anti-thrombotic activity, improving HCII-mediated thrombin inhibition, PT and aPTT almost 2-fold. Overall this study identifies an alternative source of anti-thrombotic molecules that can be easily processed offering alternatives to current anti-thrombotic agents like heparin. |
| first_indexed | 2024-04-12T19:56:49Z |
| format | Article |
| id | doaj.art-a4b856d80c974141bdfc8a459b1bea66 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:56:49Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a4b856d80c974141bdfc8a459b1bea662022-12-22T03:18:38ZengMDPI AGMarine Drugs1660-33972016-12-01151810.3390/md15010008md15010008In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing WasteHafiz Ansar Rasul Suleria0Barney M. Hines1Rama Addepalli2Wei Chen3Paul Masci4Glenda Gobe5Simone A. Osborne6School of Medicine, The University of Queensland, Translational Research Institute, Kent Street, Woolloongabba 4102, AustraliaCSIRO Agriculture, 306 Carmody Road, St Lucia 4067, AustraliaCSIRO Agriculture, 306 Carmody Road, St Lucia 4067, AustraliaCSIRO Agriculture, 306 Carmody Road, St Lucia 4067, AustraliaSchool of Medicine, The University of Queensland, Translational Research Institute, Kent Street, Woolloongabba 4102, AustraliaSchool of Medicine, The University of Queensland, Translational Research Institute, Kent Street, Woolloongabba 4102, AustraliaCSIRO Agriculture, 306 Carmody Road, St Lucia 4067, AustraliaWaste generated from the processing of marine organisms for food represents an underutilized resource that has the potential to provide bioactive molecules with pharmaceutical applications. Some of these molecules have known anti-thrombotic and anti-coagulant activities and are being investigated as alternatives to common anti-thrombotic drugs, like heparin and warfarin that have serious side effects. In the current study, extracts prepared from blacklip abalone (Haliotis rubra) processing waste, using food grade enzymes papain and bromelain, were found to contain sulphated polysaccharide with anti-thrombotic activity. Extracts were found to be enriched with sulphated polysaccharides and assessed for anti-thrombotic activity in vitro through heparin cofactor-II (HCII)-mediated inhibition of thrombin. More than 60% thrombin inhibition was observed in response to 100 μg/mL sulphated polysaccharides. Anti-thrombotic potential was further assessed as anti-coagulant activity in plasma and blood, using prothrombin time (PT), activated partial thromboplastin time (aPTT), and thromboelastography (TEG). All abalone extracts had significant activity compared with saline control. Anion exchange chromatography was used to separate extracts into fractions with enhanced anti-thrombotic activity, improving HCII-mediated thrombin inhibition, PT and aPTT almost 2-fold. Overall this study identifies an alternative source of anti-thrombotic molecules that can be easily processed offering alternatives to current anti-thrombotic agents like heparin.http://www.mdpi.com/1660-3397/15/1/8blacklip abaloneprocessing wastebioactive moleculesanti-thrombotic activity |
| spellingShingle | Hafiz Ansar Rasul Suleria Barney M. Hines Rama Addepalli Wei Chen Paul Masci Glenda Gobe Simone A. Osborne In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste Marine Drugs blacklip abalone processing waste bioactive molecules anti-thrombotic activity |
| title | In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste |
| title_full | In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste |
| title_fullStr | In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste |
| title_full_unstemmed | In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste |
| title_short | In vitro Anti-Thrombotic Activity of Extracts from Blacklip Abalone (Haliotis rubra) Processing Waste |
| title_sort | in vitro anti thrombotic activity of extracts from blacklip abalone haliotis rubra processing waste |
| topic | blacklip abalone processing waste bioactive molecules anti-thrombotic activity |
| url | http://www.mdpi.com/1660-3397/15/1/8 |
| work_keys_str_mv | AT hafizansarrasulsuleria invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste AT barneymhines invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste AT ramaaddepalli invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste AT weichen invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste AT paulmasci invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste AT glendagobe invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste AT simoneaosborne invitroantithromboticactivityofextractsfromblacklipabalonehaliotisrubraprocessingwaste |