Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate
Abstract Objectives To evaluate intermediate treatments between sodium hypochlorite and chlorhexidine gluconate irrigations for the prevention of a toxic brown precipitate in root canal therapy. Materials and Methods Thirty‐nine premolars were irrigated with 6% sodium hypochlorite and divided into e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Clinical and Experimental Dental Research |
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| Online Access: | https://doi.org/10.1002/cre2.654 |
| _version_ | 1797980830866341888 |
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| author | Vashti Bueso Neha Parikh Tanguy Terlier Julian N. Holland Nima D. Sarmast Ji Wook Jeong |
| author_facet | Vashti Bueso Neha Parikh Tanguy Terlier Julian N. Holland Nima D. Sarmast Ji Wook Jeong |
| author_sort | Vashti Bueso |
| collection | DOAJ |
| description | Abstract Objectives To evaluate intermediate treatments between sodium hypochlorite and chlorhexidine gluconate irrigations for the prevention of a toxic brown precipitate in root canal therapy. Materials and Methods Thirty‐nine premolars were irrigated with 6% sodium hypochlorite and divided into either: No intermediate treatment; Dry paper points; three different irrigations with 17% ethylenediaminetetraacetic acid, deionized water, or 5% sodium thiosulfate. 2% chlorhexidine gluconate was the final irrigant in all groups. Sectioned teeth were analyzed for brown precipitate intensity and area using stereomicroscopy and components related to para‐chloroaniline using Time‐of‐Flight Secondary Ion Mass Spectrometry (ToF‐SIMS). Results Stereomicroscopy showed that 5% STS significantly reduced brown precipitate intensity and area as compared with no intermediate irrigation (p < .05, Chi‐square, generalized linear model, and Tukey's multiple comparison tests). Utilizing ToF‐SIMS, 5% sodium thiosulfate was most effective in reducing the components representing para‐chloroaniline and chlorhexidine gluconate. Conclusion The 5% sodium thiosulfate was most effective among other intermediate treatments, assessed by stereomicroscopy and ToF‐SIMS. |
| first_indexed | 2024-04-11T06:00:02Z |
| format | Article |
| id | doaj.art-a4bc62d6c5d24c17a316d23019a320e0 |
| institution | Directory Open Access Journal |
| issn | 2057-4347 |
| language | English |
| last_indexed | 2024-04-11T06:00:02Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Experimental Dental Research |
| spelling | doaj.art-a4bc62d6c5d24c17a316d23019a320e02022-12-22T04:41:44ZengWileyClinical and Experimental Dental Research2057-43472022-12-01861591159710.1002/cre2.654Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconateVashti Bueso0Neha Parikh1Tanguy Terlier2Julian N. Holland3Nima D. Sarmast4Ji Wook Jeong5School of Dentistry The University of Texas Health Science Center at Houston Houston Texas USADepartment of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USASIMS Laboratory, Shared Equipment Authority Rice University Houston Texas USAOffice of Research, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USADepartment of Endodontics, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USADepartment of Endodontics, The University of Texas Health Science Center at Houston School of Dentistry Houston Texas USAAbstract Objectives To evaluate intermediate treatments between sodium hypochlorite and chlorhexidine gluconate irrigations for the prevention of a toxic brown precipitate in root canal therapy. Materials and Methods Thirty‐nine premolars were irrigated with 6% sodium hypochlorite and divided into either: No intermediate treatment; Dry paper points; three different irrigations with 17% ethylenediaminetetraacetic acid, deionized water, or 5% sodium thiosulfate. 2% chlorhexidine gluconate was the final irrigant in all groups. Sectioned teeth were analyzed for brown precipitate intensity and area using stereomicroscopy and components related to para‐chloroaniline using Time‐of‐Flight Secondary Ion Mass Spectrometry (ToF‐SIMS). Results Stereomicroscopy showed that 5% STS significantly reduced brown precipitate intensity and area as compared with no intermediate irrigation (p < .05, Chi‐square, generalized linear model, and Tukey's multiple comparison tests). Utilizing ToF‐SIMS, 5% sodium thiosulfate was most effective in reducing the components representing para‐chloroaniline and chlorhexidine gluconate. Conclusion The 5% sodium thiosulfate was most effective among other intermediate treatments, assessed by stereomicroscopy and ToF‐SIMS.https://doi.org/10.1002/cre2.654brown precipitationchlorohexidine gluconateintermediate irrigationsodium hypochloritesodium thiosulphate |
| spellingShingle | Vashti Bueso Neha Parikh Tanguy Terlier Julian N. Holland Nima D. Sarmast Ji Wook Jeong Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate Clinical and Experimental Dental Research brown precipitation chlorohexidine gluconate intermediate irrigation sodium hypochlorite sodium thiosulphate |
| title | Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate |
| title_full | Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate |
| title_fullStr | Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate |
| title_full_unstemmed | Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate |
| title_short | Comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate |
| title_sort | comparative evaluation of intermediate solutions in prevention of brown precipitate formed from sodium hypochlorite and chlorhexidine gluconate |
| topic | brown precipitation chlorohexidine gluconate intermediate irrigation sodium hypochlorite sodium thiosulphate |
| url | https://doi.org/10.1002/cre2.654 |
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