Microparticles: potential new contributors to the pathogenesis of systemic sclerosis?
Abstract Background Microparticles (MPs) are membrane-derived vesicles released from cells undergoing activation or apoptosis with diverse proinflammatory and prothrombotic activities, that have been implicated in the pathogenesis of systemic sclerosis (SSc). We aimed to evaluate the plasma levels o...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Advances in Rheumatology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42358-023-00299-y |
| _version_ | 1797836164740153344 |
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| author | Sandra Maximiano de Oliveira Ighor Luiz de Azevedo Teixeira Carolina Nunes França Maria Cristina de Oliveira Izar Cristiane Kayser |
| author_facet | Sandra Maximiano de Oliveira Ighor Luiz de Azevedo Teixeira Carolina Nunes França Maria Cristina de Oliveira Izar Cristiane Kayser |
| author_sort | Sandra Maximiano de Oliveira |
| collection | DOAJ |
| description | Abstract Background Microparticles (MPs) are membrane-derived vesicles released from cells undergoing activation or apoptosis with diverse proinflammatory and prothrombotic activities, that have been implicated in the pathogenesis of systemic sclerosis (SSc). We aimed to evaluate the plasma levels of platelet-derived microparticles (PMPs), endothelial cell-derived microparticles (EMPs), and monocyte-derived microparticles (MMPs) in SSc patients, and the association between MPs and the clinical features of SSc. Methods In this cross-sectional study, 70 patients with SSc and 35 age- and sex-matched healthy controls were evaluated. Clinical and nailfold capillaroscopy (NFC) data were obtained from all patients. Plasma levels of PMPs (CD42+/31+), EMPs (CD105+), and MMPs (CD14+) were quantified by flow cytometry. Results Patients were mainly females (90%), with a mean age of 48.9 years old. PMP, EMP, and MMP levels were significantly increased in SSc patients compared to controls (79.2% ± 17.3% vs. 71.0% ± 19.8%, p = 0.033; 43.5% ± 8.7% vs. 37.8% ± 10.4%, p = 0.004; and 3.5% ± 1.3% vs. 1.1% ± 0.5%, p < 0.0001, respectively). PMP levels were significantly higher in patients with positive anti-topoisomerase-I antibodies (p = 0.030) and in patients with a disease duration > 3 years (p = 0.038). EMP levels were lower in patients with a higher modified Rodnan skin score (p = 0.015), and in those with an avascular score > 1.5 in NFC (p = 0.042). Conclusion The increased levels of PMPs, EMPs and MMPs in scleroderma patients might indicate a possible role for these agents in the pathogenesis of this challenging disease. |
| first_indexed | 2024-04-09T15:05:24Z |
| format | Article |
| id | doaj.art-a4c46d10000748f18bcc3a760cb3abb0 |
| institution | Directory Open Access Journal |
| issn | 2523-3106 |
| language | English |
| last_indexed | 2024-04-09T15:05:24Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Advances in Rheumatology |
| spelling | doaj.art-a4c46d10000748f18bcc3a760cb3abb02023-04-30T11:32:09ZengBMCAdvances in Rheumatology2523-31062023-04-016311810.1186/s42358-023-00299-yMicroparticles: potential new contributors to the pathogenesis of systemic sclerosis?Sandra Maximiano de Oliveira0Ighor Luiz de Azevedo Teixeira1Carolina Nunes França2Maria Cristina de Oliveira Izar3Cristiane Kayser4Rheumatology Division, Escola Paulista de Medicina, Federal University of São Paulo – UNIFESPLaboratory of Cellular and Molecular Biology – Lipids, Atherosclerosis and Vascular Biology Section, Cardiology Division, Escola Paulista de Medicina, Universidade Federal de São Paulo – UNIFESPLaboratory of Cellular and Molecular Biology – Lipids, Atherosclerosis and Vascular Biology Section, Cardiology Division, Escola Paulista de Medicina, Universidade Federal de São Paulo – UNIFESPLaboratory of Cellular and Molecular Biology – Lipids, Atherosclerosis and Vascular Biology Section, Cardiology Division, Escola Paulista de Medicina, Universidade Federal de São Paulo – UNIFESPRheumatology Division, Escola Paulista de Medicina, Federal University of São Paulo – UNIFESPAbstract Background Microparticles (MPs) are membrane-derived vesicles released from cells undergoing activation or apoptosis with diverse proinflammatory and prothrombotic activities, that have been implicated in the pathogenesis of systemic sclerosis (SSc). We aimed to evaluate the plasma levels of platelet-derived microparticles (PMPs), endothelial cell-derived microparticles (EMPs), and monocyte-derived microparticles (MMPs) in SSc patients, and the association between MPs and the clinical features of SSc. Methods In this cross-sectional study, 70 patients with SSc and 35 age- and sex-matched healthy controls were evaluated. Clinical and nailfold capillaroscopy (NFC) data were obtained from all patients. Plasma levels of PMPs (CD42+/31+), EMPs (CD105+), and MMPs (CD14+) were quantified by flow cytometry. Results Patients were mainly females (90%), with a mean age of 48.9 years old. PMP, EMP, and MMP levels were significantly increased in SSc patients compared to controls (79.2% ± 17.3% vs. 71.0% ± 19.8%, p = 0.033; 43.5% ± 8.7% vs. 37.8% ± 10.4%, p = 0.004; and 3.5% ± 1.3% vs. 1.1% ± 0.5%, p < 0.0001, respectively). PMP levels were significantly higher in patients with positive anti-topoisomerase-I antibodies (p = 0.030) and in patients with a disease duration > 3 years (p = 0.038). EMP levels were lower in patients with a higher modified Rodnan skin score (p = 0.015), and in those with an avascular score > 1.5 in NFC (p = 0.042). Conclusion The increased levels of PMPs, EMPs and MMPs in scleroderma patients might indicate a possible role for these agents in the pathogenesis of this challenging disease.https://doi.org/10.1186/s42358-023-00299-yMicroparticlesSystemic sclerosisMicroangiopathyCapillaroscopy |
| spellingShingle | Sandra Maximiano de Oliveira Ighor Luiz de Azevedo Teixeira Carolina Nunes França Maria Cristina de Oliveira Izar Cristiane Kayser Microparticles: potential new contributors to the pathogenesis of systemic sclerosis? Advances in Rheumatology Microparticles Systemic sclerosis Microangiopathy Capillaroscopy |
| title | Microparticles: potential new contributors to the pathogenesis of systemic sclerosis? |
| title_full | Microparticles: potential new contributors to the pathogenesis of systemic sclerosis? |
| title_fullStr | Microparticles: potential new contributors to the pathogenesis of systemic sclerosis? |
| title_full_unstemmed | Microparticles: potential new contributors to the pathogenesis of systemic sclerosis? |
| title_short | Microparticles: potential new contributors to the pathogenesis of systemic sclerosis? |
| title_sort | microparticles potential new contributors to the pathogenesis of systemic sclerosis |
| topic | Microparticles Systemic sclerosis Microangiopathy Capillaroscopy |
| url | https://doi.org/10.1186/s42358-023-00299-y |
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