microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease
Abstract Background Parkinson's disease (PD) is one of the most common systemic neurodegenerative diseases and is related to the loss of dopaminergic neurons in the substantia nigra. Several studies verified that microRNA (miRNAs) targeting the Bim/Bax/caspase‐3 signaling axis is involved in th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-03-01
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| Series: | Brain and Behavior |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/brb3.2921 |
| _version_ | 1797870953852567552 |
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| author | Yufang Yao Zhiyue Zhao Fubo Zhang Na Miao Nan Wang Xin Xu Chaoping Yang |
| author_facet | Yufang Yao Zhiyue Zhao Fubo Zhang Na Miao Nan Wang Xin Xu Chaoping Yang |
| author_sort | Yufang Yao |
| collection | DOAJ |
| description | Abstract Background Parkinson's disease (PD) is one of the most common systemic neurodegenerative diseases and is related to the loss of dopaminergic neurons in the substantia nigra. Several studies verified that microRNA (miRNAs) targeting the Bim/Bax/caspase‐3 signaling axis is involved in the apoptosis of dopaminergic neurons in substantia nigra. In this study, we aimed to explore the role of miR‐221 in PD. Methods To examine the function of miR‐221 in vivo, we used a well‐established 6‐OHDA‐induced PD mouse model. Then we conducted adenovirus‐mediated miR‐221 overexpression in the PD mice. Results Our results showed that miR‐221 overexpression improved motor behavior of the PD mice. We demonstrated that overexpression of miR‐221 reduced the loss of dopaminergic neurons in the substantia nigra striatum by promoting their antioxidative and antiapoptosis capacities. Mechanistically, miR‐221 targets Bim, thus inhibiting Bim and Bax caspase‐3 mediated apoptosis signaling pathways. Conclusion Our findings suggest miR‐221 participates in the pathological process of PD and might be a potential drug target and provide new insight into PD treatment. |
| first_indexed | 2024-04-10T00:35:28Z |
| format | Article |
| id | doaj.art-a4c5999c41c94992ac377a704825b067 |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-04-10T00:35:28Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-a4c5999c41c94992ac377a704825b0672023-03-14T17:46:48ZengWileyBrain and Behavior2162-32792023-03-01133n/an/a10.1002/brb3.2921microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's diseaseYufang Yao0Zhiyue Zhao1Fubo Zhang2Na Miao3Nan Wang4Xin Xu5Chaoping Yang6Department 7 of NeurologyCangzhou Central Hospital Cangzhou Hebei ChinaCollege of Mechanical and Electrical Engineering Cangzhou Normal University Cangzhou Hebei ChinaDepartment 4 of NeurologyCangzhou Central Hospital Cangzhou Hebei ChinaDepartment 7 of NeurologyCangzhou Central Hospital Cangzhou Hebei ChinaDepartment 4 of NeurologyCangzhou Central Hospital Cangzhou Hebei ChinaDepartment 1 of Traditional Chinese MedicineCangzhou Central Hospital Cangzhou Hebei ChinaDepartment 4 of NeurologyCangzhou Central Hospital Cangzhou Hebei ChinaAbstract Background Parkinson's disease (PD) is one of the most common systemic neurodegenerative diseases and is related to the loss of dopaminergic neurons in the substantia nigra. Several studies verified that microRNA (miRNAs) targeting the Bim/Bax/caspase‐3 signaling axis is involved in the apoptosis of dopaminergic neurons in substantia nigra. In this study, we aimed to explore the role of miR‐221 in PD. Methods To examine the function of miR‐221 in vivo, we used a well‐established 6‐OHDA‐induced PD mouse model. Then we conducted adenovirus‐mediated miR‐221 overexpression in the PD mice. Results Our results showed that miR‐221 overexpression improved motor behavior of the PD mice. We demonstrated that overexpression of miR‐221 reduced the loss of dopaminergic neurons in the substantia nigra striatum by promoting their antioxidative and antiapoptosis capacities. Mechanistically, miR‐221 targets Bim, thus inhibiting Bim and Bax caspase‐3 mediated apoptosis signaling pathways. Conclusion Our findings suggest miR‐221 participates in the pathological process of PD and might be a potential drug target and provide new insight into PD treatment.https://doi.org/10.1002/brb3.2921Bax/caspase‐3 signalingBimMiR‐221Parkinson's disease |
| spellingShingle | Yufang Yao Zhiyue Zhao Fubo Zhang Na Miao Nan Wang Xin Xu Chaoping Yang microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease Brain and Behavior Bax/caspase‐3 signaling Bim MiR‐221 Parkinson's disease |
| title | microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease |
| title_full | microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease |
| title_fullStr | microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease |
| title_full_unstemmed | microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease |
| title_short | microRNA‐221 rescues the loss of dopaminergic neurons in a mouse model of Parkinson's disease |
| title_sort | microrna 221 rescues the loss of dopaminergic neurons in a mouse model of parkinson s disease |
| topic | Bax/caspase‐3 signaling Bim MiR‐221 Parkinson's disease |
| url | https://doi.org/10.1002/brb3.2921 |
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