Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support
Capillary electrophoresis (CE) is a very powerful analytical technique, consuming very small quantities of samples and solvents. Generally, a UV detector is coupled with CE but for the analysis of drugs in biological fluids, it is necessary to use a more sensitive and selective detector su...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | deu |
| Published: |
Swiss Chemical Society
2008-04-01
|
| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://chimia.ch/chimia/article/view/4463 |
| Summary: | Capillary electrophoresis (CE) is a very powerful analytical technique, consuming very small quantities of samples and solvents. Generally, a UV detector is coupled with CE but for the analysis of drugs in biological fluids, it is necessary to use a more sensitive and selective detector
such as laser induced fluorescence (LIF). This detector was used to analyze various amphetamine derivatives which do not present native fluorescence and therefore a derivatization tag was employed to produce a fluorescent adduct. Fluorescamine, a fluorogene agent, was selected because it reacts
with primary amines of amphetamines to give a fluorescent compound negatively charged, which can be analyzed by CZE-LIF. Taking into account the presence of many interfering compounds in biological fluids, analysis of these compounds requires a sample preparation prior to the analysis. Solid
phase extraction (SPE) was selected and the derivatization step included. Analysis of independent plasma samples (n = 6) exhibited a relative standard deviation (RSD) of 8%. A relative recovery of 99% was observed for amphetamine, used as a model compound.
|
|---|---|
| ISSN: | 0009-4293 2673-2424 |