Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support
Capillary electrophoresis (CE) is a very powerful analytical technique, consuming very small quantities of samples and solvents. Generally, a UV detector is coupled with CE but for the analysis of drugs in biological fluids, it is necessary to use a more sensitive and selective detector su...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2008-04-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://chimia.ch/chimia/article/view/4463 |
| _version_ | 1811214220401836032 |
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| author | Ludovic Gremaud Julie Schappler Jean-Luc Veuthey Claude Rohrbasser Serge Rudaz |
| author_facet | Ludovic Gremaud Julie Schappler Jean-Luc Veuthey Claude Rohrbasser Serge Rudaz |
| author_sort | Ludovic Gremaud |
| collection | DOAJ |
| description |
Capillary electrophoresis (CE) is a very powerful analytical technique, consuming very small quantities of samples and solvents. Generally, a UV detector is coupled with CE but for the analysis of drugs in biological fluids, it is necessary to use a more sensitive and selective detector
such as laser induced fluorescence (LIF). This detector was used to analyze various amphetamine derivatives which do not present native fluorescence and therefore a derivatization tag was employed to produce a fluorescent adduct. Fluorescamine, a fluorogene agent, was selected because it reacts
with primary amines of amphetamines to give a fluorescent compound negatively charged, which can be analyzed by CZE-LIF. Taking into account the presence of many interfering compounds in biological fluids, analysis of these compounds requires a sample preparation prior to the analysis. Solid
phase extraction (SPE) was selected and the derivatization step included. Analysis of independent plasma samples (n = 6) exhibited a relative standard deviation (RSD) of 8%. A relative recovery of 99% was observed for amphetamine, used as a model compound.
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| first_indexed | 2024-04-12T06:00:40Z |
| format | Article |
| id | doaj.art-a4c61a43f186424aad0436b24e6cd7d6 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-04-12T06:00:40Z |
| publishDate | 2008-04-01 |
| publisher | Swiss Chemical Society |
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| series | CHIMIA |
| spelling | doaj.art-a4c61a43f186424aad0436b24e6cd7d62022-12-22T03:45:03ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242008-04-0162410.2533/chimia.2008.210Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction SupportLudovic GremaudJulie SchapplerJean-Luc VeutheyClaude RohrbasserSerge Rudaz Capillary electrophoresis (CE) is a very powerful analytical technique, consuming very small quantities of samples and solvents. Generally, a UV detector is coupled with CE but for the analysis of drugs in biological fluids, it is necessary to use a more sensitive and selective detector such as laser induced fluorescence (LIF). This detector was used to analyze various amphetamine derivatives which do not present native fluorescence and therefore a derivatization tag was employed to produce a fluorescent adduct. Fluorescamine, a fluorogene agent, was selected because it reacts with primary amines of amphetamines to give a fluorescent compound negatively charged, which can be analyzed by CZE-LIF. Taking into account the presence of many interfering compounds in biological fluids, analysis of these compounds requires a sample preparation prior to the analysis. Solid phase extraction (SPE) was selected and the derivatization step included. Analysis of independent plasma samples (n = 6) exhibited a relative standard deviation (RSD) of 8%. A relative recovery of 99% was observed for amphetamine, used as a model compound. https://chimia.ch/chimia/article/view/4463AmphetaminesCapillary zone electrophoresisDerivatizationLaser induced fluorescenceSolid phase extraction |
| spellingShingle | Ludovic Gremaud Julie Schappler Jean-Luc Veuthey Claude Rohrbasser Serge Rudaz Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support CHIMIA Amphetamines Capillary zone electrophoresis Derivatization Laser induced fluorescence Solid phase extraction |
| title | Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support |
| title_full | Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support |
| title_fullStr | Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support |
| title_full_unstemmed | Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support |
| title_short | Analysis of Amphetamine Derivatives in Plasma Using Capillary Zone Electrophoresis Coupled with Laser-Induced Fluorescence after Derivatization on Solid-Phase Extraction Support |
| title_sort | analysis of amphetamine derivatives in plasma using capillary zone electrophoresis coupled with laser induced fluorescence after derivatization on solid phase extraction support |
| topic | Amphetamines Capillary zone electrophoresis Derivatization Laser induced fluorescence Solid phase extraction |
| url | https://chimia.ch/chimia/article/view/4463 |
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