Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR)
Background: Epidemic and endemic cholera is a major public health problem for many countries. Aim of this study was to evaluate AP-PCR for investigation of clonal relatedness among the strains of Vibrio cholerae recovered from an outbreak occurred in different parts of Iran in 2005. Methods: The stu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2008-07-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/9153.pdf&manuscript_id=9153 |
| _version_ | 1818608269441105920 |
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| author | M Izadi M Hamidian A Karami N Sadeghifard MR Pourshafie R Ranjbar M Parzadeh N Jonaidi |
| author_facet | M Izadi M Hamidian A Karami N Sadeghifard MR Pourshafie R Ranjbar M Parzadeh N Jonaidi |
| author_sort | M Izadi |
| collection | DOAJ |
| description | Background: Epidemic and endemic cholera is a major public health problem for many countries. Aim of this study was to evaluate AP-PCR for investigation of clonal relatedness among the strains of Vibrio cholerae recovered from an outbreak occurred in different parts of Iran in 2005. Methods: The study was conducted during the cholera outbreak occurred in some of provinces in Iran in summer 2005. Bacterial isolation and identification was carried out according to the standard bacteriological methods. Arbitrarily primed PCR (AP-PCR) used to study the genetic relatedness between the V.cholerae isolates. Results: Thirty-nine isolates of V.cholerae O1 were identified. All isolates belonged to serotype Inaba. AP-PCR could differentiate the isolates into five groups. AP-PCR cluster types 1 and 2 were the most prevalent groups, accounting for 36% and 41%, respectively, of V.cholerae isolates. Conclusion: The most of epidemic strains of V.cholerae O1 isolated in the year 2005 could be attributed to two predominant clusters including AP-PCR cluster types 1 and 2 accounting for more than 77% of isolates. In conclusion, a few epidemic clones were responsible for the apparently epidemic occurrence of cholera in provinces studied. |
| first_indexed | 2024-12-16T14:39:58Z |
| format | Article |
| id | doaj.art-a4c88eddbc3d46c68ce9cf2723f2ee2a |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-16T14:39:58Z |
| publishDate | 2008-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-a4c88eddbc3d46c68ce9cf2723f2ee2a2022-12-21T22:27:59ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852008-07-013728387Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR)M IzadiM HamidianA KaramiN SadeghifardMR PourshafieR RanjbarM ParzadehN JonaidiBackground: Epidemic and endemic cholera is a major public health problem for many countries. Aim of this study was to evaluate AP-PCR for investigation of clonal relatedness among the strains of Vibrio cholerae recovered from an outbreak occurred in different parts of Iran in 2005. Methods: The study was conducted during the cholera outbreak occurred in some of provinces in Iran in summer 2005. Bacterial isolation and identification was carried out according to the standard bacteriological methods. Arbitrarily primed PCR (AP-PCR) used to study the genetic relatedness between the V.cholerae isolates. Results: Thirty-nine isolates of V.cholerae O1 were identified. All isolates belonged to serotype Inaba. AP-PCR could differentiate the isolates into five groups. AP-PCR cluster types 1 and 2 were the most prevalent groups, accounting for 36% and 41%, respectively, of V.cholerae isolates. Conclusion: The most of epidemic strains of V.cholerae O1 isolated in the year 2005 could be attributed to two predominant clusters including AP-PCR cluster types 1 and 2 accounting for more than 77% of isolates. In conclusion, a few epidemic clones were responsible for the apparently epidemic occurrence of cholera in provinces studied.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/9153.pdf&manuscript_id=9153Vibrio Cholerae O1AP-PCRMolecular Epidemiology |
| spellingShingle | M Izadi M Hamidian A Karami N Sadeghifard MR Pourshafie R Ranjbar M Parzadeh N Jonaidi Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR) Iranian Journal of Public Health Vibrio Cholerae O1 AP-PCR Molecular Epidemiology |
| title | Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR) |
| title_full | Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR) |
| title_fullStr | Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR) |
| title_full_unstemmed | Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR) |
| title_short | Molecular Characterization of Epidemic Isolates of Vibrio Cholerae O1 by Arbitrarily Primed PCR (AP-PCR) |
| title_sort | molecular characterization of epidemic isolates of vibrio cholerae o1 by arbitrarily primed pcr ap pcr |
| topic | Vibrio Cholerae O1 AP-PCR Molecular Epidemiology |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/9153.pdf&manuscript_id=9153 |
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