Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs
Abstract This study aimed to determine the effects of melatonin administration on testicular vascular perfusion in relation to steroid hormones and semen characteristics in dogs. The study included 12 normospermic German shepherd dogs (weighed 35 ± 0.5 kg and aged 4 ± 0.5 years). Males received a si...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-022-03487-y |
| _version_ | 1811319303200309248 |
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| author | Ali Salama Elshymaa A. Abdelnaby Ibrahim A. Emam Mohamed Fathi |
| author_facet | Ali Salama Elshymaa A. Abdelnaby Ibrahim A. Emam Mohamed Fathi |
| author_sort | Ali Salama |
| collection | DOAJ |
| description | Abstract This study aimed to determine the effects of melatonin administration on testicular vascular perfusion in relation to steroid hormones and semen characteristics in dogs. The study included 12 normospermic German shepherd dogs (weighed 35 ± 0.5 kg and aged 4 ± 0.5 years). Males received a single melatonin administration (melatonin dimethyl sulfoxide + corn oil via subcutaneous route; MEL; n = 6), while the rest of the animals served as controls (dimethyl sulfoxide + corn oil; Control; n = 6). Males were subjected to routine examination on days -15, 0, 15, 30, 45, and 60. All examined dogs were subjected to Doppler screening, semen collection, and blood sampling. The MEL group showed a significant (P < 0.05) elevation in semen volume, concentration, percentage of sperm motility, and total sperm × 106 / ejaculate compared to other control males. Doppler indices as resistance (RI) and pulsatility (PI) indices declined (P < 0.05) from D 30 (1.02 ± 0.01) until day 60 (0.87 ± 0.02) of treatment. In MEL males, the peak systolic point of velocity (PSV; cm/sec) of the testicular artery elevated (P < 0.05) on day 60 (20.15 ± 0.99) compared to its value on day 0 (17.39 ± 1.84). On D 60, the levels of testosterone (T), estradiol 17-ß (E2), and nitric oxide (NO) elevated (P < 0.05). A negative correlation was detected between testicular volume, scrotal circumference (SC), T levels, Doppler indices, and velocities. In conclusion, single melatonin administration could improve testicular vascularization via increasing Doppler velocities and intratesticular colored areas. In addition, it could improve semen picture and steroids (T and E2) and nitric oxide. |
| first_indexed | 2024-04-13T12:39:12Z |
| format | Article |
| id | doaj.art-a4cac64abdc84f6dbdada1ffb68b2d61 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-13T12:39:12Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-a4cac64abdc84f6dbdada1ffb68b2d612022-12-22T02:46:33ZengBMCBMC Veterinary Research1746-61482022-11-0118111010.1186/s12917-022-03487-ySingle melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogsAli Salama0Elshymaa A. Abdelnaby1Ibrahim A. Emam2Mohamed Fathi3Theriogenology Department, Faculty of Veterinary Medicine, Cairo UniversityTheriogenology Department, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo UniversityTheriogenology Department, Faculty of Veterinary Medicine, Cairo UniversityAbstract This study aimed to determine the effects of melatonin administration on testicular vascular perfusion in relation to steroid hormones and semen characteristics in dogs. The study included 12 normospermic German shepherd dogs (weighed 35 ± 0.5 kg and aged 4 ± 0.5 years). Males received a single melatonin administration (melatonin dimethyl sulfoxide + corn oil via subcutaneous route; MEL; n = 6), while the rest of the animals served as controls (dimethyl sulfoxide + corn oil; Control; n = 6). Males were subjected to routine examination on days -15, 0, 15, 30, 45, and 60. All examined dogs were subjected to Doppler screening, semen collection, and blood sampling. The MEL group showed a significant (P < 0.05) elevation in semen volume, concentration, percentage of sperm motility, and total sperm × 106 / ejaculate compared to other control males. Doppler indices as resistance (RI) and pulsatility (PI) indices declined (P < 0.05) from D 30 (1.02 ± 0.01) until day 60 (0.87 ± 0.02) of treatment. In MEL males, the peak systolic point of velocity (PSV; cm/sec) of the testicular artery elevated (P < 0.05) on day 60 (20.15 ± 0.99) compared to its value on day 0 (17.39 ± 1.84). On D 60, the levels of testosterone (T), estradiol 17-ß (E2), and nitric oxide (NO) elevated (P < 0.05). A negative correlation was detected between testicular volume, scrotal circumference (SC), T levels, Doppler indices, and velocities. In conclusion, single melatonin administration could improve testicular vascularization via increasing Doppler velocities and intratesticular colored areas. In addition, it could improve semen picture and steroids (T and E2) and nitric oxide.https://doi.org/10.1186/s12917-022-03487-yCanineDopplerMelatoninSpermTesticular arteryVolume |
| spellingShingle | Ali Salama Elshymaa A. Abdelnaby Ibrahim A. Emam Mohamed Fathi Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs BMC Veterinary Research Canine Doppler Melatonin Sperm Testicular artery Volume |
| title | Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs |
| title_full | Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs |
| title_fullStr | Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs |
| title_full_unstemmed | Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs |
| title_short | Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs |
| title_sort | single melatonin injection enhances the testicular artery hemodynamic reproductive hormones and semen parameters in german shepherd dogs |
| topic | Canine Doppler Melatonin Sperm Testicular artery Volume |
| url | https://doi.org/10.1186/s12917-022-03487-y |
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