Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process
Abstract Background Research on aging is growing as the elderly make up a greater share of the population, focusing on reversing and inhibiting the aging process. The exhaustion and senescence of stem cells are the fundamental drivers behind aging. β-Carotene has been depicted to have many biologica...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | Cellular & Molecular Biology Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11658-022-00389-7 |
| _version_ | 1797996018160107520 |
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| author | Wei V. Zheng Wang Xu Yaqin Li Jie Qin Tao Zhou Dezhi Li Yanwei Xu Xianyi Cheng Yu Xiong Zaizhong Chen |
| author_facet | Wei V. Zheng Wang Xu Yaqin Li Jie Qin Tao Zhou Dezhi Li Yanwei Xu Xianyi Cheng Yu Xiong Zaizhong Chen |
| author_sort | Wei V. Zheng |
| collection | DOAJ |
| description | Abstract Background Research on aging is growing as the elderly make up a greater share of the population, focusing on reversing and inhibiting the aging process. The exhaustion and senescence of stem cells are the fundamental drivers behind aging. β-Carotene has been depicted to have many biological functions, and we speculate that it may have an anti-aging effect. Methods Firstly, the anti-aging property of β-carotene was investigated in vitro using mesenchymal stem cells (MSCs) induced by H2O2. The anti-aging effect was characterized using Western-bloting, confocal laser scanning microscopy, indirect immunofluorescence, and immunohistochemistry. The anti-aging property was also tested in vivo using aged mice. Results The in vitro experiment revealed that β-carotene could relieve the aging of MSCs, as evidenced by a series of aging marker molecules such as p16 and p21. β-Carotene appeared to inhibit aging by regulating the KAT7-P15 signaling axis. The in vivo experiment revealed that β-carotene treatment has significantly down-regulated the aging level of tissues and organs. Conclusions In this work, we explored the anti-aging effect of β-carotene in vivo and in vitro. The experimental results indicate that β-carotene may be an important potential anti-aging molecule, which can be used as a drug or in functional food to treat aging in the future. |
| first_indexed | 2024-04-11T10:10:59Z |
| format | Article |
| id | doaj.art-a4d1090f79b148bdbf67d53cf7ac9ecf |
| institution | Directory Open Access Journal |
| issn | 1425-8153 1689-1392 |
| language | English |
| last_indexed | 2024-04-11T10:10:59Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Cellular & Molecular Biology Letters |
| spelling | doaj.art-a4d1090f79b148bdbf67d53cf7ac9ecf2022-12-22T04:30:06ZengBMCCellular & Molecular Biology Letters1425-81531689-13922022-10-0127112110.1186/s11658-022-00389-7Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress processWei V. Zheng0Wang Xu1Yaqin Li2Jie Qin3Tao Zhou4Dezhi Li5Yanwei Xu6Xianyi Cheng7Yu Xiong8Zaizhong Chen9Intervention and Cell Therapy Center, Peking University Shenzhen HospitalCollege of Veterinary Medicine, Northwest A&F UniversityDepartment of Infectious Disease, Peking University Shenzhen HospitalScientific and Resaerch Dept., Peking University Shenzhen HospitalIntervention and Cell Therapy Center, Peking University Shenzhen HospitalIntervention and Cell Therapy Center, Peking University Shenzhen HospitalIntervention and Cell Therapy Center, Peking University Shenzhen HospitalIntervention and Cell Therapy Center, Peking University Shenzhen HospitalIntervention and Cell Therapy Center, Peking University Shenzhen HospitalIntervention and Cell Therapy Center, Peking University Shenzhen HospitalAbstract Background Research on aging is growing as the elderly make up a greater share of the population, focusing on reversing and inhibiting the aging process. The exhaustion and senescence of stem cells are the fundamental drivers behind aging. β-Carotene has been depicted to have many biological functions, and we speculate that it may have an anti-aging effect. Methods Firstly, the anti-aging property of β-carotene was investigated in vitro using mesenchymal stem cells (MSCs) induced by H2O2. The anti-aging effect was characterized using Western-bloting, confocal laser scanning microscopy, indirect immunofluorescence, and immunohistochemistry. The anti-aging property was also tested in vivo using aged mice. Results The in vitro experiment revealed that β-carotene could relieve the aging of MSCs, as evidenced by a series of aging marker molecules such as p16 and p21. β-Carotene appeared to inhibit aging by regulating the KAT7-P15 signaling axis. The in vivo experiment revealed that β-carotene treatment has significantly down-regulated the aging level of tissues and organs. Conclusions In this work, we explored the anti-aging effect of β-carotene in vivo and in vitro. The experimental results indicate that β-carotene may be an important potential anti-aging molecule, which can be used as a drug or in functional food to treat aging in the future.https://doi.org/10.1186/s11658-022-00389-7β-CaroteneAgingStem cellsKAT7-P15 signaling axis |
| spellingShingle | Wei V. Zheng Wang Xu Yaqin Li Jie Qin Tao Zhou Dezhi Li Yanwei Xu Xianyi Cheng Yu Xiong Zaizhong Chen Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process Cellular & Molecular Biology Letters β-Carotene Aging Stem cells KAT7-P15 signaling axis |
| title | Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process |
| title_full | Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process |
| title_fullStr | Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process |
| title_full_unstemmed | Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process |
| title_short | Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process |
| title_sort | anti aging effect of β carotene through regulating the kat7 p15 signaling axis inflammation and oxidative stress process |
| topic | β-Carotene Aging Stem cells KAT7-P15 signaling axis |
| url | https://doi.org/10.1186/s11658-022-00389-7 |
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