SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation
Abstract In mice, retrotransposon‐associated long noncoding RNAs (lncRNA) play important regulatory roles in pre‐implantation development; however, it is largely unknown whether they function in the pre‐implantation development in pigs. The current study aims to screen for retrotransposon‐associated...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202307505 |
| _version_ | 1797356419179085824 |
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| author | Tianyao He Jinyu Peng Shu Yang Dongsong Liu Shuang Gao Yanlong Zhu Zhuang Chai Byeong Chun Lee Renyue Wei Jiaqiang Wang Zhonghua Liu Jun‐Xue Jin |
| author_facet | Tianyao He Jinyu Peng Shu Yang Dongsong Liu Shuang Gao Yanlong Zhu Zhuang Chai Byeong Chun Lee Renyue Wei Jiaqiang Wang Zhonghua Liu Jun‐Xue Jin |
| author_sort | Tianyao He |
| collection | DOAJ |
| description | Abstract In mice, retrotransposon‐associated long noncoding RNAs (lncRNA) play important regulatory roles in pre‐implantation development; however, it is largely unknown whether they function in the pre‐implantation development in pigs. The current study aims to screen for retrotransposon‐associated lncRNA in porcine early embryos and identifies a porcine 8‐cell embryo‐specific SINE‐associated nuclear long noncoding RNA named SAWPA. SAWPA is essential for porcine embryonic development as depletion of SAWPA results in a developmental arrest at the 8‐cell stage, accompanied by the inhibition of the JNK‐MAPK signaling pathway. Mechanistically, SAWPA works in trans as a transcription factor for JNK through the formation of an RNA‐protein complex with HNRNPA1 and MED8 binding the SINE elements upstream of JNK. Therefore, as the first functional SINE‐associated long noncoding RNAs in pigs, SAWPA provides novel insights for the mechanism research on retrotransposons in mammalian pre‐implantation development. |
| first_indexed | 2024-03-08T14:26:23Z |
| format | Article |
| id | doaj.art-a4d7b250d8b24ead842f2ec2e2888209 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-08T14:26:23Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-a4d7b250d8b24ead842f2ec2e28882092024-01-13T04:23:06ZengWileyAdvanced Science2198-38442024-01-01112n/an/a10.1002/advs.202307505SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome ActivationTianyao He0Jinyu Peng1Shu Yang2Dongsong Liu3Shuang Gao4Yanlong Zhu5Zhuang Chai6Byeong Chun Lee7Renyue Wei8Jiaqiang Wang9Zhonghua Liu10Jun‐Xue Jin11Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaDepartment of Theriogenology and Biotechnology College of Veterinary Medicine Seoul National University Seoul 08826 South KoreaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaKey Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province College of Life Science Northeast Agricultural University Harbin 150030 P. R. ChinaAbstract In mice, retrotransposon‐associated long noncoding RNAs (lncRNA) play important regulatory roles in pre‐implantation development; however, it is largely unknown whether they function in the pre‐implantation development in pigs. The current study aims to screen for retrotransposon‐associated lncRNA in porcine early embryos and identifies a porcine 8‐cell embryo‐specific SINE‐associated nuclear long noncoding RNA named SAWPA. SAWPA is essential for porcine embryonic development as depletion of SAWPA results in a developmental arrest at the 8‐cell stage, accompanied by the inhibition of the JNK‐MAPK signaling pathway. Mechanistically, SAWPA works in trans as a transcription factor for JNK through the formation of an RNA‐protein complex with HNRNPA1 and MED8 binding the SINE elements upstream of JNK. Therefore, as the first functional SINE‐associated long noncoding RNAs in pigs, SAWPA provides novel insights for the mechanism research on retrotransposons in mammalian pre‐implantation development.https://doi.org/10.1002/advs.202307505embryo developmentlncRNAshort‐interspersed elementstranscriptional regulationZGA |
| spellingShingle | Tianyao He Jinyu Peng Shu Yang Dongsong Liu Shuang Gao Yanlong Zhu Zhuang Chai Byeong Chun Lee Renyue Wei Jiaqiang Wang Zhonghua Liu Jun‐Xue Jin SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation Advanced Science embryo development lncRNA short‐interspersed elements transcriptional regulation ZGA |
| title | SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation |
| title_full | SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation |
| title_fullStr | SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation |
| title_full_unstemmed | SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation |
| title_short | SINE‐Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation |
| title_sort | sine associated lncrna sawpa regulates porcine zygotic genome activation |
| topic | embryo development lncRNA short‐interspersed elements transcriptional regulation ZGA |
| url | https://doi.org/10.1002/advs.202307505 |
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