Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis
Laboratory tools for diagnosing taeniosis/cysticercosis in non-endemic countries are available; however, there is little data on their performance. To provide information on the sensitivity, specificity, and reproducibility of these tools, inter-laboratory studies were organized within the EU COST-A...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1173 |
| _version_ | 1797532136708767744 |
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| author | María Ángeles Gómez-Morales Patrizio Pezzotti Alessandra Ludovisi Belgees Boufana Pierre Dorny Titia Kortbeek Joachim Blocher Veronika Schmidt Marco Amati Sarah Gabriël Edoardo Pozio Andrea Sylvia Winkler The Ring Trial Participants |
| author_facet | María Ángeles Gómez-Morales Patrizio Pezzotti Alessandra Ludovisi Belgees Boufana Pierre Dorny Titia Kortbeek Joachim Blocher Veronika Schmidt Marco Amati Sarah Gabriël Edoardo Pozio Andrea Sylvia Winkler The Ring Trial Participants |
| author_sort | María Ángeles Gómez-Morales |
| collection | DOAJ |
| description | Laboratory tools for diagnosing taeniosis/cysticercosis in non-endemic countries are available; however, there is little data on their performance. To provide information on the sensitivity, specificity, and reproducibility of these tools, inter-laboratory studies were organized within the EU COST-Action CYSTINET (TD1302). Two serological and one coprological Ring Trials (RTs) were organized to test a panel of human-derived sera and stool samples using assays routinely conducted by the participating laboratories to detect <i>Taenia</i> spp. infections. Four Western blots (WBs) and five ELISAs were used by nine laboratories for cysticercosis diagnosis. In the first serological RT, the overall sensitivity was 67.6% (95% CI, 59.1–75.4), whereas specificity was 97% (95% CI, 89.8–99.6). WBs recorded the best accuracy. A second serological RT was organized, to assess the three tests most frequently used during the first RT. Two out of six laboratories performed all the three tests. The overall sensitivity and specificity were 52.8% (95% CI, 42.8–62.7) and 98.1% (95% CI, 93.2–99.7), respectively. Laboratory performance strongly affected test results. Twelve laboratories participated in the coprological RT using conventional microscopy and six laboratories used molecular assays. Traditional diagnosis by microscopy yielded better results than molecular diagnosis. This may have been influenced by the lack of standardization of molecular tests across participating laboratories. |
| first_indexed | 2024-03-10T10:54:50Z |
| format | Article |
| id | doaj.art-a4d98f96bc1945338953f2eb5173b239 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:54:50Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a4d98f96bc1945338953f2eb5173b2392023-11-21T22:00:07ZengMDPI AGMicroorganisms2076-26072021-05-0196117310.3390/microorganisms9061173Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/CysticercosisMaría Ángeles Gómez-Morales0Patrizio Pezzotti1Alessandra Ludovisi2Belgees Boufana3Pierre Dorny4Titia Kortbeek5Joachim Blocher6Veronika Schmidt7Marco Amati8Sarah Gabriël9Edoardo Pozio10Andrea Sylvia Winkler11The Ring Trial ParticipantsDepartment of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyVeterinary Helminthology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerpen, BelgiumCenter IDS, National Institute of Public Health and the Environment, RIVM, 3720 Bilthoven, The NetherlandsInstitute of Acute Neurology, Academic Teaching Hospital Feldkirch, Carinagasse 47, 6800 Feldkirch, AustriaCenter for Global Health, Department of Neurology, Technical University of Munich, 81675 Munich, GermanyDepartment of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyDepartment of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyCenter for Global Health, Department of Neurology, Technical University of Munich, 81675 Munich, GermanyLaboratory tools for diagnosing taeniosis/cysticercosis in non-endemic countries are available; however, there is little data on their performance. To provide information on the sensitivity, specificity, and reproducibility of these tools, inter-laboratory studies were organized within the EU COST-Action CYSTINET (TD1302). Two serological and one coprological Ring Trials (RTs) were organized to test a panel of human-derived sera and stool samples using assays routinely conducted by the participating laboratories to detect <i>Taenia</i> spp. infections. Four Western blots (WBs) and five ELISAs were used by nine laboratories for cysticercosis diagnosis. In the first serological RT, the overall sensitivity was 67.6% (95% CI, 59.1–75.4), whereas specificity was 97% (95% CI, 89.8–99.6). WBs recorded the best accuracy. A second serological RT was organized, to assess the three tests most frequently used during the first RT. Two out of six laboratories performed all the three tests. The overall sensitivity and specificity were 52.8% (95% CI, 42.8–62.7) and 98.1% (95% CI, 93.2–99.7), respectively. Laboratory performance strongly affected test results. Twelve laboratories participated in the coprological RT using conventional microscopy and six laboratories used molecular assays. Traditional diagnosis by microscopy yielded better results than molecular diagnosis. This may have been influenced by the lack of standardization of molecular tests across participating laboratories.https://www.mdpi.com/2076-2607/9/6/1173cysticercosisneurocysticercosis<i>Taenia</i> spp.diagnosistaeniosiscollaborative study |
| spellingShingle | María Ángeles Gómez-Morales Patrizio Pezzotti Alessandra Ludovisi Belgees Boufana Pierre Dorny Titia Kortbeek Joachim Blocher Veronika Schmidt Marco Amati Sarah Gabriël Edoardo Pozio Andrea Sylvia Winkler The Ring Trial Participants Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis Microorganisms cysticercosis neurocysticercosis <i>Taenia</i> spp. diagnosis taeniosis collaborative study |
| title | Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis |
| title_full | Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis |
| title_fullStr | Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis |
| title_full_unstemmed | Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis |
| title_short | Collaborative Studies for the Detection of <em>Taenia</em> spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis |
| title_sort | collaborative studies for the detection of em taenia em spp infections in humans within cystinet the european network on taeniosis cysticercosis |
| topic | cysticercosis neurocysticercosis <i>Taenia</i> spp. diagnosis taeniosis collaborative study |
| url | https://www.mdpi.com/2076-2607/9/6/1173 |
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